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CA11 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CA11
Gene Name:  carbonic anhydrase XI
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482729.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296387

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 984nts         Open reading frame : 23 to 984
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGGGGGCTGCAGCTCGTCTGAGCGCCCCTCGAGCGCT
GGTACTCTGGGCTGCACTGGGGGCAGCAGCTCACATCGGACCAGCACCTGACCCCGAGGA
CTGGTGGAGCTACAAGGATAATCTCCAGGGAAACTTCGTGCCAGGGCCTCCTTTCTGGGG
CCTGGTGAATGCAGCGTGGAGTCTGTGTGCTGTGGGGAAGCGGCAGAGCCCCGTGGATGT
GGAGCTGAAGAGGGTTCTTTATGACCCCTTTCTGCCCCCATTAAGGCTCAGCACTGGAGG
AGAGAAGCTCCGGGGAACCTTGTACAACACCGGCCGACATGTCTCCTTCCTGCCTGCACC
CCGACCTGTGGTCAATGTGTCTGGAGGTCCCCTCCTTTACAGCCACCGACTCAGTGAACT
GCGGCTGCTGTTTGGAGCTCGCGACGGAGCCGGCTCGGAACATCAGATCAACCACCAGGG
CTTCTCTGCTGAGGTGCAGCTCATTCACTTCAACCAGGAACTCTACGGGAATTTCAGCGC
TGCCTCCCGCGGCCCCAATGGCCTGGCCATTCTCAGCCTCTTTGTCAACGTTGCCAGTAC
CTCTAACCCATTCCTCAGTCGCCTCCTTAACCGCGACACCATCACTCGCATCTCCTACAA
GAATGATGCCTACTTTCTTCAAGACCTGAGCCTGGAGCTCCTGTTCCCTGAATCCTTCGG
CTTCATCACCTATCAGGGCTCTCTCAGCACCCCGCCCTGCTCCGAGACTGTCACCTGGAT
CCTCATTGACCGGGCCCTCAATATCACCTCCCTTCAGATGCACTCCCTGAGACTCCTGAG
CCAGAATCCTCCATCTCAGATCTTCCAGAGCCTCAGCGGTAACAGCCGGCCCCTGCAGCC
CTTGGCCCACAGGGCACTGAGGGGCAACAGGGACCCCCGGCACCCCGAGAGGCGCTGCCG
AGGCCCCAACTACCGCCTGCATGTGGATGGTGTCCCCCATGGTCGCTGGATCCACCCAGC
TTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1082
Start on reference sequence 96
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGAATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA11
Symbol Nomenclature : CA11
SYNONYM : CARPX1
Designation : CA-RP II|CA-XI|CARP XI|CARP-2|carbonic anhydrase-related protein 11|carbonic anhydrase-related protein 2|carbonic anhydrase-related protein XI
Full Nomenclature : carbonic anhydrase XI
GENEID : 770
Locus Tag : UNQ211/PRO237
GI : 190690270
GenBank Accession : EU832425
HGNC : 1370
MIM : 604644
Vega : OTTHUMG00000183320
Target GenBank: BC002662.2

 

Reference Sequence Alignment

  

HsCD00296387      GTACAAAAAAGCAGGCTCCACCATGGGGGCTGCAGCTCGTCTGAGCGCCCCTCGAGCGCT
NM_001217.3       ----------------------ATGGGGGCTGCAGCTCGTCTGAGCGCCCCTCGAGCGCT
                                        **************************************

HsCD00296387      GGTACTCTGGGCTGCACTGGGGGCAGCAGCTCACATCGGACCAGCACCTGACCCCGAGGA
NM_001217.3       GGTACTCTGGGCTGCACTGGGGGCAGCAGCTCACATCGGACCAGCACCTGACCCCGAGGA
                  ************************************************************

HsCD00296387      CTGGTGGAGCTACAAGGATAATCTCCAGGGAAACTTCGTGCCAGGGCCTCCTTTCTGGGG
NM_001217.3       CTGGTGGAGCTACAAGGATAATCTCCAGGGAAACTTCGTGCCAGGGCCTCCTTTCTGGGG
                  ************************************************************

HsCD00296387      CCTGGTGAATGCAGCGTGGAGTCTGTGTGCTGTGGGGAAGCGGCAGAGCCCCGTGGATGT
NM_001217.3       CCTGGTGAATGCAGCGTGGAGTCTGTGTGCTGTGGGGAAGCGGCAGAGCCCCGTGGATGT
                  ************************************************************

HsCD00296387      GGAGCTGAAGAGGGTTCTTTATGACCCCTTTCTGCCCCCATTAAGGCTCAGCACTGGAGG
NM_001217.3       GGAGCTGAAGAGGGTTCTTTATGACCCCTTTCTGCCCCCATTAAGGCTCAGCACTGGAGG
                  ************************************************************

HsCD00296387      AGAGAAGCTCCGGGGAACCTTGTACAACACCGGCCGACATGTCTCCTTCCTGCCTGCACC
NM_001217.3       AGAGAAGCTCCGGGGAACCTTGTACAACACCGGCCGACATGTCTCCTTCCTGCCTGCACC
                  ************************************************************

HsCD00296387      CCGACCTGTGGTCAATGTGTCTGGAGGTCCCCTCCTTTACAGCCACCGACTCAGTGAACT
NM_001217.3       CCGACCTGTGGTCAATGTGTCTGGAGGTCCCCTCCTTTACAGCCACCGACTCAGTGAACT
                  ************************************************************

HsCD00296387      GCGGCTGCTGTTTGGAGCTCGCGACGGAGCCGGCTCGGAACATCAGATCAACCACCAGGG
NM_001217.3       GCGGCTGCTGTTTGGAGCTCGCGACGGAGCCGGCTCGGAACATCAGATCAACCACCAGGG
                  ************************************************************

HsCD00296387      CTTCTCTGCTGAGGTGCAGCTCATTCACTTCAACCAGGAACTCTACGGGAATTTCAGCGC
NM_001217.3       CTTCTCTGCTGAGGTGCAGCTCATTCACTTCAACCAGGAACTCTACGGGAATTTCAGCGC
                  ************************************************************

HsCD00296387      TGCCTCCCGCGGCCCCAATGGCCTGGCCATTCTCAGCCTCTTTGTCAACGTTGCCAGTAC
NM_001217.3       TGCCTCCCGCGGCCCCAATGGCCTGGCCATTCTCAGCCTCTTTGTCAACGTTGCCAGTAC
                  ************************************************************

HsCD00296387      CTCTAACCCATTCCTCAGTCGCCTCCTTAACCGCGACACCATCACTCGCATCTCCTACAA
NM_001217.3       CTCTAACCCATTCCTCAGTCGCCTCCTTAACCGCGACACCATCACTCGCATCTCCTACAA
                  ************************************************************

HsCD00296387      GAATGATGCCTACTTTCTTCAAGACCTGAGCCTGGAGCTCCTGTTCCCTGAATCCTTCGG
NM_001217.3       GAATGATGCCTACTTTCTTCAAGACCTGAGCCTGGAGCTCCTGTTCCCTGAATCCTTCGG
                  ************************************************************

HsCD00296387      CTTCATCACCTATCAGGGCTCTCTCAGCACCCCGCCCTGCTCCGAGACTGTCACCTGGAT
NM_001217.3       CTTCATCACCTATCAGGGCTCTCTCAGCACCCCGCCCTGCTCCGAGACTGTCACCTGGAT
                  ************************************************************

HsCD00296387      CCTCATTGACCGGGCCCTCAATATCACCTCCCTTCAGATGCACTCCCTGAGACTCCTGAG
NM_001217.3       CCTCATTGACCGGGCCCTCAATATCACCTCCCTTCAGATGCACTCCCTGAGACTCCTGAG
                  ************************************************************

HsCD00296387      CCAGAATCCTCCATCTCAGATCTTCCAGAGCCTCAGCGGTAACAGCCGGCCCCTGCAGCC
NM_001217.3       CCAGAATCCTCCATCTCAGATCTTCCAGAGCCTCAGCGGTAACAGCCGGCCCCTGCAGCC
                  ************************************************************

HsCD00296387      CTTGGCCCACAGGGCACTGAGGGGCAACAGGGACCCCCGGCACCCCGAGAGGCGCTGCCG
NM_001217.3       CTTGGCCCACAGGGCACTGAGGGGCAACAGGGACCCCCGGCACCCCGAGAGGCGCTGCCG
                  ************************************************************

HsCD00296387      AGGCCCCAACTACCGCCTGCATGTGGATGGTGTCCCCCATGGTCGCTGGATCCACCCAGC
NM_001217.3       AGGCCCCAACTACCGCCTGCATGTGGATGGTGTCCCCCATGGTCGCTGA-----------
                  ************************************************.           

HsCD00296387      TTTCTTGTAC
NM_001217.3       ----------
                            

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
UniProt : O75493
HPRD : 05225

Clone

Cloning Information : 482729
HIP Master Clone ID : 400457
IMAGE ID : 100067454
Original Clone ID : FLH482729.01L

Organism


TAX_ID : 9606
Species Specific ID: 770

Genome

Chromosome : 19
Map Location : 19q13.3
Ensembl : ENSG00000063180

Reagents

Labome : CA11-antibody