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VISA (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MAVS
Gene Name:  mitochondrial antiviral signaling protein
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482817.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23246644
Title: A polymerase subunit PB2 possesses overlapping binding sites for polymerase subunit PB1 and human MAVS proteins
Authors: The ORFeome Collaboration
HsCD00296475

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1623nts         Open reading frame : 23 to 1623
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGCCGTTTGCTGAAGACAAGACCTATAAGTATATCTG
CCGCAATTTCAGCAATTTTTGCAATGTGGATGTTGTAGAGATTCTGCCTTACCTGCCCTG
CCTCACAGCAAGAGACCAGGATCGACTGCGGGCCACCTGCACACTCTCAGGGAACCGGGA
CACCCTCTGGCATCTCTTCAATACCCTTCAGCGGCGGCCCGGCTGGGTGGAGTACTTCAT
TGCGGCACTGAGGGGCTGTGAGCTAGTTGATCTCGCGGACGAAGTGGCCTCTGTCTACGA
GAGCTACCAGCCTCGGACCTCGGACCGTCCCCCAGACCCACTGGAGCCACCGTCACTTCC
TGCTGAGAGGCCAGGGCCCCCCACACCTGCTGCGGCCCACAGCATCCCCTACAACAGCTG
CAGAGAGAAGGAGCCAAGTTACCCCATGCCTGTCCAGGAGACCCAGGCGCCAGAGTCCCC
AGGAGAGAATTCAGAGCAAGCCCTGCAGACGCTCAGCCCCAGAGCCATCCCAAGGAATCC
AGATGGTGGCCCCCTGGAGTCCTCCTCTGACCTGGCAGCCCTCAGCCCTCTGACCTCCAG
CGGGCATCAGGAGAAGGACACAGAACTGGGCAGTACCCACACAGCAGGTGCGACCTCCAG
CCTCACACCATCCCGTGGGCCTGTGTCTCCATCTGTCTCCTTCCAGCCCCTGGCCCGTTC
CACCCCCAGGGCAAGCCGCTTGCCTGGACCCACAGGGTCAGTTGTATCTACTGGCACCTC
CTTCTCCTCCTCATCCCCTGGCTTGGCCTCTGCAGGGGCTGCAGAGGGTAAACAGGGTGC
AGAGAGTGACCAGGCCGAGCCTATCATCTGCTCCAGTGGGGCAGAGGCACCTGCCAACTC
TCTGCCCTCCAAAGTGCCTACCACCTTGATGCCTGTGAACACAGTGGCCCTGAAAGTGCC
TGCCAACCCAGCATCTGTCAGCACAGTGCCCTCCAAGTTGCCAACTAGCTCAAAGCCCCC
TGGTGCAGTGCCTTCTAATGCGCTCACCAATCCAGCACCATCCAAATTGCCCATCAACTC
AACCCGTGCTGGCATGGTGCCATCCAAAGTGCCTACTAGCATGGTGCTCACCAAGGTGTC
TGCCAGCACAGTCCCCACTGACGGGAGCAGCAGAAATGAGGAGACCCCAGCAGCTCCAAC
ACCCGCCGGCGCCACTGGAGGCAGCTCAGCCTGGCTAGACAGCAGCTTTGAGAATAGGGG
CCTTGGGTCGGAGCTGAGTAAGCCTGGCGTGCTGGCATCCCAGGTAGACAGCCCGTTCTC
GGGCTGCTTCGAGGATCTTGCCATCAGTGCCAGCACCTCCTTGGGCATGGGGCCCTGCCA
TGGCCCAGAGGAGAATGAGTATAAGTCCGAGGGCACCTTTGGGATCCACGTGGCTGAGAA
CCCCAGCATCCAGCTCCTGGAGGGCAACCCTGGGCCACCTGCGGACCCGGATGGCGGCCC
CAGGCCACAAGCCGACCGGAAGTTCCAGGAGAGGGAGGTGCCATGCCACAGGCCCTCACC
TGGGGCTCTGTGGCTCCAGGTGGCTGTGACAGGGGTGCTGGTAGTCACACTCCTGGTGGT
GCTGTACCGGCGGCGTCTGCACTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1729
Start on reference sequence 107
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MAVS
Symbol Nomenclature : MAVS
SYNONYM : CARDIF; IPS-1; IPS1; VISA
Designation : CARD adapter inducing interferon beta|CARD adaptor inducing IFN-beta|IFN-B promoter stimulator 1|interferon beta promoter stimulator protein 1|mitochondrial antiviral-signaling protein|putative NF-kappa-B-activating protein 031N|virus-induced signaling adaptor|virus-induced-signaling adapter
Full Nomenclature : mitochondrial antiviral signaling protein
GENEID : 57506
GI : 190691794
GenBank Accession : EU832500
HGNC : 29233
MIM : 609676
Target GenBank: BC044952.1

 

Reference Sequence Alignment

  

HsCD00296475      GTACAAAAAAGCAGGCTCCACCATGCCGTTTGCTGAAGACAAGACCTATAAGTATATCTG
NM_020746.4       ----------------------ATGCCGTTTGCTGAAGACAAGACCTATAAGTATATCTG
                                        **************************************

HsCD00296475      CCGCAATTTCAGCAATTTTTGCAATGTGGATGTTGTAGAGATTCTGCCTTACCTGCCCTG
NM_020746.4       CCGCAATTTCAGCAATTTTTGCAATGTGGATGTTGTAGAGATTCTGCCTTACCTGCCCTG
                  ************************************************************

HsCD00296475      CCTCACAGCAAGAGACCAGGATCGACTGCGGGCCACCTGCACACTCTCAGGGAACCGGGA
NM_020746.4       CCTCACAGCAAGAGACCAGGATCGACTGCGGGCCACCTGCACACTCTCAGGGAACCGGGA
                  ************************************************************

HsCD00296475      CACCCTCTGGCATCTCTTCAATACCCTTCAGCGGCGGCCCGGCTGGGTGGAGTACTTCAT
NM_020746.4       CACCCTCTGGCATCTCTTCAATACCCTTCAGCGGCGGCCCGGCTGGGTGGAGTACTTCAT
                  ************************************************************

HsCD00296475      TGCGGCACTGAGGGGCTGTGAGCTAGTTGATCTCGCGGACGAAGTGGCCTCTGTCTACGA
NM_020746.4       TGCGGCACTGAGGGGCTGTGAGCTAGTTGATCTCGCGGACGAAGTGGCCTCTGTCTACCA
                  ********************************************************** *

HsCD00296475      GAGCTACCAGCCTCGGACCTCGGACCGTCCCCCAGACCCACTGGAGCCACCGTCACTTCC
NM_020746.4       GAGCTACCAGCCTCGGACCTCGGACCGTCCCCCAGACCCACTGGAGCCACCGTCACTTCC
                  ************************************************************

HsCD00296475      TGCTGAGAGGCCAGGGCCCCCCACACCTGCTGCGGCCCACAGCATCCCCTACAACAGCTG
NM_020746.4       TGCTGAGAGGCCAGGGCCCCCCACACCTGCTGCGGCCCACAGCATCCCCTACAACAGCTG
                  ************************************************************

HsCD00296475      CAGAGAGAAGGAGCCAAGTTACCCCATGCCTGTCCAGGAGACCCAGGCGCCAGAGTCCCC
NM_020746.4       CAGAGAGAAGGAGCCAAGTTACCCCATGCCTGTCCAGGAGACCCAGGCGCCAGAGTCCCC
                  ************************************************************

HsCD00296475      AGGAGAGAATTCAGAGCAAGCCCTGCAGACGCTCAGCCCCAGAGCCATCCCAAGGAATCC
NM_020746.4       AGGAGAGAATTCAGAGCAAGCCCTGCAGACGCTCAGCCCCAGAGCCATCCCAAGGAATCC
                  ************************************************************

HsCD00296475      AGATGGTGGCCCCCTGGAGTCCTCCTCTGACCTGGCAGCCCTCAGCCCTCTGACCTCCAG
NM_020746.4       AGATGGTGGCCCCCTGGAGTCCTCCTCTGACCTGGCAGCCCTCAGCCCTCTGACCTCCAG
                  ************************************************************

HsCD00296475      CGGGCATCAGGAGAAGGACACAGAACTGGGCAGTACCCACACAGCAGGTGCGACCTCCAG
NM_020746.4       CGGGCATCAGGAGCAGGACACAGAACTGGGCAGTACCCACACAGCAGGTGCGACCTCCAG
                  *************.**********************************************

HsCD00296475      CCTCACACCATCCCGTGGGCCTGTGTCTCCATCTGTCTCCTTCCAGCCCCTGGCCCGTTC
NM_020746.4       CCTCACACCATCCCGTGGGCCTGTGTCTCCATCTGTCTCCTTCCAGCCCCTGGCCCGTTC
                  ************************************************************

HsCD00296475      CACCCCCAGGGCAAGCCGCTTGCCTGGACCCACAGGGTCAGTTGTATCTACTGGCACCTC
NM_020746.4       CACCCCCAGGGCAAGCCGCTTGCCTGGACCCACAGGGTCAGTTGTATCTACTGGCACCTC
                  ************************************************************

HsCD00296475      CTTCTCCTCCTCATCCCCTGGCTTGGCCTCTGCAGGGGCTGCAGAGGGTAAACAGGGTGC
NM_020746.4       CTTCTCCTCCTCATCCCCTGGCTTGGCCTCTGCAGGGGCTGCAGAGGGTAAACAGGGTGC
                  ************************************************************

HsCD00296475      AGAGAGTGACCAGGCCGAGCCTATCATCTGCTCCAGTGGGGCAGAGGCACCTGCCAACTC
NM_020746.4       AGAGAGTGACCAGGCCGAGCCTATCATCTGCTCCAGTGGGGCAGAGGCACCTGCCAACTC
                  ************************************************************

HsCD00296475      TCTGCCCTCCAAAGTGCCTACCACCTTGATGCCTGTGAACACAGTGGCCCTGAAAGTGCC
NM_020746.4       TCTGCCCTCCAAAGTGCCTACCACCTTGATGCCTGTGAACACAGTGGCCCTGAAAGTGCC
                  ************************************************************

HsCD00296475      TGCCAACCCAGCATCTGTCAGCACAGTGCCCTCCAAGTTGCCAACTAGCTCAAAGCCCCC
NM_020746.4       TGCCAACCCAGCATCTGTCAGCACAGTGCCCTCCAAGTTGCCAACTAGCTCAAAGCCCCC
                  ************************************************************

HsCD00296475      TGGTGCAGTGCCTTCTAATGCGCTCACCAATCCAGCACCATCCAAATTGCCCATCAACTC
NM_020746.4       TGGTGCAGTGCCTTCTAATGCGCTCACCAATCCAGCACCATCCAAATTGCCCATCAACTC
                  ************************************************************

HsCD00296475      AACCCGTGCTGGCATGGTGCCATCCAAAGTGCCTACTAGCATGGTGCTCACCAAGGTGTC
NM_020746.4       AACCCGTGCTGGCATGGTGCCATCCAAAGTGCCTACTAGCATGGTGCTCACCAAGGTGTC
                  ************************************************************

HsCD00296475      TGCCAGCACAGTCCCCACTGACGGGAGCAGCAGAAATGAGGAGACCCCAGCAGCTCCAAC
NM_020746.4       TGCCAGCACAGTCCCCACTGACGGGAGCAGCAGAAATGAGGAGACCCCAGCAGCTCCAAC
                  ************************************************************

HsCD00296475      ACCCGCCGGCGCCACTGGAGGCAGCTCAGCCTGGCTAGACAGCAGCTTTGAGAATAGGGG
NM_020746.4       ACCCGCCGGCGCCACTGGAGGCAGCTCAGCCTGGCTAGACAGCAGCTCTGAGAATAGGGG
                  *********************************************** ************

HsCD00296475      CCTTGGGTCGGAGCTGAGTAAGCCTGGCGTGCTGGCATCCCAGGTAGACAGCCCGTTCTC
NM_020746.4       CCTTGGGTCGGAGCTGAGTAAGCCTGGCGTGCTGGCATCCCAGGTAGACAGCCCGTTCTC
                  ************************************************************

HsCD00296475      GGGCTGCTTCGAGGATCTTGCCATCAGTGCCAGCACCTCCTTGGGCATGGGGCCCTGCCA
NM_020746.4       GGGCTGCTTCGAGGATCTTGCCATCAGTGCCAGCACCTCCTTGGGCATGGGGCCCTGCCA
                  ************************************************************

HsCD00296475      TGGCCCAGAGGAGAATGAGTATAAGTCCGAGGGCACCTTTGGGATCCACGTGGCTGAGAA
NM_020746.4       TGGCCCAGAGGAGAATGAGTATAAGTCCGAGGGCACCTTTGGGATCCACGTGGCTGAGAA
                  ************************************************************

HsCD00296475      CCCCAGCATCCAGCTCCTGGAGGGCAACCCTGGGCCACCTGCGGACCCGGATGGCGGCCC
NM_020746.4       CCCCAGCATCCAGCTCCTGGAGGGCAACCCTGGGCCACCTGCGGACCCGGATGGCGGCCC
                  ************************************************************

HsCD00296475      CAGGCCACAAGCCGACCGGAAGTTCCAGGAGAGGGAGGTGCCATGCCACAGGCCCTCACC
NM_020746.4       CAGGCCACAAGCCGACCGGAAGTTCCAGGAGAGGGAGGTGCCATGCCACAGGCCCTCACC
                  ************************************************************

HsCD00296475      TGGGGCTCTGTGGCTCCAGGTGGCTGTGACAGGGGTGCTGGTAGTCACACTCCTGGTGGT
NM_020746.4       TGGGGCTCTGTGGCTCCAGGTGGCTGTGACAGGGGTGCTGGTAGTCACACTCCTGGTGGT
                  ************************************************************

HsCD00296475      GCTGTACCGGCGGCGTCTGCACTGAATCCACCCAGCTTTCTTGTAC
NM_020746.4       GCTGTACCGGCGGCGTCTGCACTAG---------------------
                  ***********************..                     

Pathways

Reactome : Immune System
Reactome : Innate Immune System
Reactome : NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
Reactome : Negative regulators of RIG-I/MDA5 signaling
Reactome : RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
Reactome : TRAF3-dependent IRF activation pathway
Reactome : TRAF6 mediated IRF7 activation
Reactome : TRAF6 mediated NF-kB activation

Protein

UniProt : Q7Z434
HPRD : 13847

Clone

Cloning Information : 482817
HIP Master Clone ID : 400545
IMAGE ID : 100067529
Original Clone ID : FLH482817.01X

Organism


TAX_ID : 9606
Species Specific ID: 57506

Genome

Chromosome : 20
Map Location : 20p13

Reagents

Labome : MAVS-antibody