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TSG101 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TSG101
Gene Name:  tumor susceptibility 101
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH117330.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002966

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1173nts         Open reading frame : 1 to 1173
Reference Sequence Alignment

ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
ACTATCCTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1257
Start on reference sequence 85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TSG101
Symbol Nomenclature : TSG101
SYNONYM : TSG10; VPS23
Designation : ESCRT-I complex subunit TSG101|tumor susceptibility gene 10|tumor susceptibility gene 101|tumor susceptibility gene 101 protein|tumor susceptibility protein
Full Nomenclature : tumor susceptibility 101
GENEID : 7251
GI : 60655268
GenBank Accession : AY889407
HGNC : 15971
MIM : 601387
Vega : OTTHUMG00000167725
Target GenBank: BC002487

 

Reference Sequence Alignment

  

HsCD00002966      ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
NM_006292.3       ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
                  ************************************************************

HsCD00002966      ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
NM_006292.3       ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
                  ************************************************************

HsCD00002966      TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
NM_006292.3       TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
                  ************************************************************

HsCD00002966      GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
NM_006292.3       GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
                  ************************************************************

HsCD00002966      CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
NM_006292.3       CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
                  ************************************************************

HsCD00002966      AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
NM_006292.3       AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
                  ************************************************************

HsCD00002966      CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
NM_006292.3       CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
                  ************************************************************

HsCD00002966      GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
NM_006292.3       GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
                  ************************************************************

HsCD00002966      ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
NM_006292.3       ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
                  ************************************************************

HsCD00002966      AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
NM_006292.3       AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
                  ************************************************************

HsCD00002966      AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
NM_006292.3       AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
                  ************************************************************

HsCD00002966      ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
NM_006292.3       ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
                  ************************************************************

HsCD00002966      CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
NM_006292.3       CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
                  ************************************************************

HsCD00002966      GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
NM_006292.3       GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
                  ************************************************************

HsCD00002966      GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
NM_006292.3       GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
                  ************************************************************

HsCD00002966      GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
NM_006292.3       GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
                  ************************************************************

HsCD00002966      ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
NM_006292.3       ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
                  ************************************************************

HsCD00002966      ACTATCCTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
NM_006292.3       ACTATCTTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
                  ****** *****************************************************

HsCD00002966      AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
NM_006292.3       AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
                  ************************************************************

HsCD00002966      AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA
NM_006292.3       AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA
                  *********************************

Pathways

NCI : Glucocorticoid receptor regulatory network
NCI : Internalization of ErbB1
Reactome : Assembly Of The HIV Virion
Reactome : Budding and maturation of HIV virion
Reactome : Disease
Reactome : Endosomal Sorting Complex Required For Transport (ESCRT)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : Late Phase of HIV Life Cycle
Reactome : Membrane Trafficking
Reactome : Membrane binding and targetting of GAG proteins

Clone

Cloning Information : 117330
HIP Master Clone ID : 93176
Original Clone ID : FLH117330.01X

Organism


TAX_ID : 9606
Species Specific ID: 7251

Genome

Chromosome : 11
Map Location : 11p15
Ensembl : ENSG00000074319

Reagents

Labome : TSG101-antibody