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KBTBD6 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KBTBD6
Gene Name:  kelch repeat and BTB (POZ) domain containing 6
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH483068.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296726

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2022nts         Open reading frame : 23 to 2022
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGCAGTCCCGGGAAGACGCCCCGCGCTCTCGCCGCCT
AGCCAGTCCCCGTGGTGGGAAGCGGCCCAAGAAGATTCACAAACCCACAGTTTCGGCCTT
TTTCACGGGTCCAGAGGAATTAAAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCT
CAAGTCCTTCTACGATGCGCGGCTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGG
CAGCGGGCCTGGCACGGGTCGCCTGTTCCCCTGCAACCGCAATGTGCTGGCCGCGGCATG
TCCCTACTTCAAGAGCATGTTCACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGAC
CATGCACGATGTGGACGCCGAGTCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCG
TGTGTCTCTCAGTGAGGCCAACGTGGAGCGCCTGTACGCGGCCTCCGACATGCTACAGCT
GGAATATGTGCGGGAAGCCTGTGCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTG
CACCGCCATCCTCAAGTTTGCAGATGCCTTTGGCCATCGCAAGCTGCGATCCCAGGCCCA
GTCCTATATAGCTCAGAACTTCAAGCAACTCAGCCACATGGGTTCAATTCGGGAGGAGAC
TCTAGCAGATCTGACCCTGGCCCAGCTGCTGGCTGTCCTGCGCTTGGATAGTCTGGACGT
GGAGAGTGAGCAGACAGTGTGCCATGTGGCAGTGCAGTGGCTGGAGGCTGCTCCCAAAGA
GCGGGGTCCCAGTGCTGCAGAAGTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGA
AGATCAGGACTACTTAGAAGGGCTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGA
CGTTATTGAAGGGGCCCTGCAGATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCC
AGTGCCAAACAGCAGCAGCAGCAGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAA
TCCACCCCAGAGACTGGGTATGTGTGCCAAGGAGATGGTGATCTTCTTTGGACACCCCAG
AGATCCCTTTCTCTGCTGTGATCCATACTCGGGGGACCTTTACAAAGTGCCGTCACCTTT
GACCTGTCTGGCTCACACTAGGACTGTCACCACTCTAGCTGTCTGTATCTCTCCTGACCA
TGACATCTATCTAGCTGCTCAGCCCAGGACAGACCTCTGGGTGTATAAACCAGCTCAGAA
TAGTTGGCAGCAACTTGCAGATCGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCT
CAATGGCTATATCTACATTTTGGGGGGGCGAGACCCTATTACTGGAGTTAAGTTGAAGGA
AGTGGAATGCTACAATGTTAAGAGAAACCAGTGGGCATTGGTGGCTCCACTGCCCCATTC
TTTTTTATCCTTTGACCTAATGGTAATTCGAGACTATCTCTATGCTCTCAACAGTAAGCG
CATGTTCTGTTATGATCCTAGCCACAATATGTGGCTGAAGTGCGTTTCTCTGAAGCGCAA
TGACTTTCAGGAAGCCTGCGTCTTCAATGAGGAGATCTATTGTATCTGTGATATCCCAGT
CATGAAGGTCTACAACCCAGTTAGGGCAGAATGGAGGCAAATGAATAATATTCCCTTGGT
CTCAGAGACCAACAACTACAGAATTATCAAGCATGGCCAAAAATTGTTGCTCATCACCTC
TCGCACCCCACAGTGGAAAAAGAACCGGGTGACTGTGTATGAATATGATATTAGGGGAGA
CCAATGGATTAATATAGGTACCACATTAGGCCTCTTGCAGTTTGATTCTAACTTTTTTTG
CCTCTCTGCTCGTGTTTATCCTTCCTGCCTTGAACCTGGTCAGAGTTTCCTCACTGAAGA
AGAAGAAATACCAAGTGAGTCTAGCACTGAATGGGACTTAGGTGGATTCAGTGAGCCAGA
CTCTGAGTCAGGAAGTTCAAGTTCTCTTTCTGATGATGATTTTTGGGTGCGTGTAGCGCC
TCAGTGGATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2075
Start on reference sequence 51
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGAATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD6
Symbol Nomenclature : KBTBD6
Designation : kelch repeat and BTB domain-containing protein 6
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 6
GENEID : 89890
GI : 190690464
GenBank Accession : EU832605
HGNC : 25340
Vega : OTTHUMG00000016786
Target GenBank: BC051349.1

 

Reference Sequence Alignment

  

HsCD00296726      GTACAAAAAAGCAGGCTCCACCATGCAGTCCCGGGAAGACGCCCCGCGCTCTCGCCGCCT
NM_152903.4       ----------------------ATGCAGTCCCGGGAAGACGCCCCGCGCTCTCGCCGCCT
                                        **************************************

HsCD00296726      AGCCAGTCCCCGTGGTGGGAAGCGGCCCAAGAAGATTCACAAACCCACAGTTTCGGCCTT
NM_152903.4       AGCCAGTCCCCGTGGTGGGAAGCGGCCCAAGAAGATTCACAAACCCACAGTTTCGGCCTT
                  ************************************************************

HsCD00296726      TTTCACGGGTCCAGAGGAATTAAAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCT
NM_152903.4       TTTCACGGGTCCAGAGGAATTAAAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCT
                  ************************************************************

HsCD00296726      CAAGTCCTTCTACGATGCGCGGCTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGG
NM_152903.4       CAAGTCCTTCTACGATGCGCGGCTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGG
                  ************************************************************

HsCD00296726      CAGCGGGCCTGGCACGGGTCGCCTGTTCCCCTGCAACCGCAATGTGCTGGCCGCGGCATG
NM_152903.4       CAGCGGGCCTGGCACGGGTCGCCTGTTCCCCTGCAACCGCAATGTGCTGGCCGCGGCATG
                  ************************************************************

HsCD00296726      TCCCTACTTCAAGAGCATGTTCACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGAC
NM_152903.4       TCCCTACTTCAAGAGCATGTTCACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGAC
                  ************************************************************

HsCD00296726      CATGCACGATGTGGACGCCGAGTCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCG
NM_152903.4       CATGCACGATGTGGACGCCGAGTCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCG
                  ************************************************************

HsCD00296726      TGTGTCTCTCAGTGAGGCCAACGTGGAGCGCCTGTACGCGGCCTCCGACATGCTACAGCT
NM_152903.4       TGTGTCTCTCAGTGAGGCCAACGTGGAGCGCCTGTACGCGGCCTCCGACATGCTACAGCT
                  ************************************************************

HsCD00296726      GGAATATGTGCGGGAAGCCTGTGCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTG
NM_152903.4       GGAATATGTGCGGGAAGCCTGTGCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTG
                  ************************************************************

HsCD00296726      CACCGCCATCCTCAAGTTTGCAGATGCCTTTGGCCATCGCAAGCTGCGATCCCAGGCCCA
NM_152903.4       CACCGCCATCCTCAAGTTTGCAGATGCCTTTGGCCATCGCAAGCTGCGATCCCAGGCCCA
                  ************************************************************

HsCD00296726      GTCCTATATAGCTCAGAACTTCAAGCAACTCAGCCACATGGGTTCAATTCGGGAGGAGAC
NM_152903.4       GTCCTATATAGCTCAGAACTTCAAGCAACTCAGCCACATGGGTTCAATTCGGGAGGAGAC
                  ************************************************************

HsCD00296726      TCTAGCAGATCTGACCCTGGCCCAGCTGCTGGCTGTCCTGCGCTTGGATAGTCTGGACGT
NM_152903.4       TCTAGCAGATCTGACCCTGGCCCAGCTGCTGGCTGTCCTGCGCTTGGATAGTCTGGACGT
                  ************************************************************

HsCD00296726      GGAGAGTGAGCAGACAGTGTGCCATGTGGCAGTGCAGTGGCTGGAGGCTGCTCCCAAAGA
NM_152903.4       GGAGAGTGAGCAGACAGTGTGCCATGTGGCAGTGCAGTGGCTGGAGGCTGCTCCCAAAGA
                  ************************************************************

HsCD00296726      GCGGGGTCCCAGTGCTGCAGAAGTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGA
NM_152903.4       GCGGGGTCCCAGTGCTGCAGAAGTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGA
                  ************************************************************

HsCD00296726      AGATCAGGACTACTTAGAAGGGCTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGA
NM_152903.4       AGATCAGGACTACTTAGAAGGGCTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGA
                  ************************************************************

HsCD00296726      CGTTATTGAAGGGGCCCTGCAGATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCC
NM_152903.4       CGTTATTGAAGGGGCCCTGCAGATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCC
                  ************************************************************

HsCD00296726      AGTGCCAAACAGCAGCAGCAGCAGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAA
NM_152903.4       AGTGCCAAACAGCAGCAGCAGCAGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAA
                  ************************************************************

HsCD00296726      TCCACCCCAGAGACTGGGTATGTGTGCCAAGGAGATGGTGATCTTCTTTGGACACCCCAG
NM_152903.4       TCCACCCCAGAGACTGGGTATGTGTGCCAAGGAGATGGTGATCTTCTTTGGACACCCCAG
                  ************************************************************

HsCD00296726      AGATCCCTTTCTCTGCTGTGATCCATACTCGGGGGACCTTTACAAAGTGCCGTCACCTTT
NM_152903.4       AGATCCCTTTCTCTGCTGTGATCCATACTCGGGGGACCTTTACAAAGTGCCGTCACCTTT
                  ************************************************************

HsCD00296726      GACCTGTCTGGCTCACACTAGGACTGTCACCACTCTAGCTGTCTGTATCTCTCCTGACCA
NM_152903.4       GACCTGTCTGGCTCACACTAGGACTGTCACCACTCTAGCTGTCTGTATCTCTCCTGACCA
                  ************************************************************

HsCD00296726      TGACATCTATCTAGCTGCTCAGCCCAGGACAGACCTCTGGGTGTATAAACCAGCTCAGAA
NM_152903.4       TGACATCTATCTAGCTGCTCAGCCCAGGACAGACCTCTGGGTGTATAAACCAGCTCAGAA
                  ************************************************************

HsCD00296726      TAGTTGGCAGCAACTTGCAGATCGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCT
NM_152903.4       TAGTTGGCAGCAACTTGCAGATCGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCT
                  ************************************************************

HsCD00296726      CAATGGCTATATCTACATTTTGGGGGGGCGAGACCCTATTACTGGAGTTAAGTTGAAGGA
NM_152903.4       CAATGGCTATATCTACATTTTGGGGGGGCGAGACCCTATTACTGGAGTTAAGTTGAAGGA
                  ************************************************************

HsCD00296726      AGTGGAATGCTACAATGTTAAGAGAAACCAGTGGGCATTGGTGGCTCCACTGCCCCATTC
NM_152903.4       AGTGGAATGCTACAATGTTAAGAGAAACCAGTGGGCATTGGTGGCTCCACTGCCCCATTC
                  ************************************************************

HsCD00296726      TTTTTTATCCTTTGACCTAATGGTAATTCGAGACTATCTCTATGCTCTCAACAGTAAGCG
NM_152903.4       TTTTTTATCCTTTGACCTAATGGTAATTCGAGACTATCTCTATGCTCTCAACAGTAAGCG
                  ************************************************************

HsCD00296726      CATGTTCTGTTATGATCCTAGCCACAATATGTGGCTGAAGTGCGTTTCTCTGAAGCGCAA
NM_152903.4       CATGTTCTGTTATGATCCTAGCCACAATATGTGGCTGAAGTGCGTTTCTCTGAAGCGCAA
                  ************************************************************

HsCD00296726      TGACTTTCAGGAAGCCTGCGTCTTCAATGAGGAGATCTATTGTATCTGTGATATCCCAGT
NM_152903.4       TGACTTTCAGGAAGCCTGCGTCTTCAATGAGGAGATCTATTGTATCTGTGATATCCCAGT
                  ************************************************************

HsCD00296726      CATGAAGGTCTACAACCCAGTTAGGGCAGAATGGAGGCAAATGAATAATATTCCCTTGGT
NM_152903.4       CATGAAGGTCTACAACCCAGTTAGGGCAGAATGGAGGCAAATGAATAATATTCCCTTGGT
                  ************************************************************

HsCD00296726      CTCAGAGACCAACAACTACAGAATTATCAAGCATGGCCAAAAATTGTTGCTCATCACCTC
NM_152903.4       CTCAGAGACCAACAACTACAGAATTATCAAGCATGGCCAAAAATTGTTGCTCATCACCTC
                  ************************************************************

HsCD00296726      TCGCACCCCACAGTGGAAAAAGAACCGGGTGACTGTGTATGAATATGATATTAGGGGAGA
NM_152903.4       TCGCACCCCACAGTGGAAAAAGAACCGGGTGACTGTGTATGAATATGATATTAGGGGAGA
                  ************************************************************

HsCD00296726      CCAATGGATTAATATAGGTACCACATTAGGCCTCTTGCAGTTTGATTCTAACTTTTTTTG
NM_152903.4       CCAATGGATTAATATAGGTACCACATTAGGCCTCTTGCAGTTTGATTCTAACTTTTTTTG
                  ************************************************************

HsCD00296726      CCTCTCTGCTCGTGTTTATCCTTCCTGCCTTGAACCTGGTCAGAGTTTCCTCACTGAAGA
NM_152903.4       CCTCTCTGCTCGTGTTTATCCTTCCTGCCTTGAACCTGGTCAGAGTTTCCTCACTGAAGA
                  ************************************************************

HsCD00296726      AGAAGAAATACCAAGTGAGTCTAGCACTGAATGGGACTTAGGTGGATTCAGTGAGCCAGA
NM_152903.4       AGAAGAAATACCAAGTGAGTCTAGCACTGAATGGGACTTAGGTGGATTCAGTGAGCCAGA
                  ************************************************************

HsCD00296726      CTCTGAGTCAGGAAGTTCAAGTTCTCTTTCTGATGATGATTTTTGGGTGCGTGTAGCGCC
NM_152903.4       CTCTGAGTCAGGAAGTTCAAGTTCTCTTTCTGATGATGATTTTTGGGTGCGTGTAGCGCC
                  ************************************************************

HsCD00296726      TCAGTGGATCCACCCAGCTTTCTTGTAC
NM_152903.4       TCAGTGA---------------------
                  ******.                     

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
UniProt : Q86V97
HPRD : 13758

Clone

Cloning Information : 483068
HIP Master Clone ID : 400796
IMAGE ID : 100067634
Original Clone ID : FLH483068.01L

Organism


TAX_ID : 9606
Species Specific ID: 89890

Genome

Chromosome : 13
Map Location : 13q14.11
Ensembl : ENSG00000165572

Reagents

Labome : KBTBD6-antibody