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PRKACB (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PRKACB
Gene Name:  protein kinase, cAMP-dependent, catalytic, beta
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH483094.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
HsCD00296752

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1197nts         Open reading frame : 23 to 1197
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGGCAGCTTATAGAGAACCACCTTGTAACCAGTATAC
AGGTACAACTACAGCTCTTCAGAAATTGGAAGGTTTTGCTAGCCGGTTATTTCATAGACA
CTCTAAAGGTACTGCACATGATCAGAAAACAGCTCTGGAAAATGACAGCCTTCATTTCTC
TGAGCATACTGCCTTATGGGACAGATCAATGAAAGAGTTTCTAGCCAAAGCCAAAGAAGA
CTTTTTGAAAAAATGGGAGAATCCAACTCAGAATAATGCCGGACTTGAAGATTTTGAAAG
GAAAAAAACCCTTGGAACAGGTTCATTTGGAAGAGTCGTGTTGGTAAAACACAAAGCCAC
TGAACAGTATTATGCCATGAAGATCTTAGATAAGCAGAAGGTTGTTAAACTGAAGCAAAT
AGAGCATACTTTGAATGAGAAAAGAATATTACAGGCAGTGAATTTTCCTTTCCTTGTTCG
ACTGGAGTATGCTTTTAAGGATAATTCTAATTTATACATGGTTATGGAATATGTCCCTGG
GGGTGAGATGTTTTCACATCTAAGAAGAATTGGAAGGTTCAGTGAGCCCCATGCACGGTT
CTATGCAGCTCAGATAGTGCTAACATTCGAGTACCTCCATTCACTAGACCTCATCTACAG
AGATCTAAAACCTGAAAATCTCTTAATTGACCATCAAGGCTATATCCAGGTCACAGACTT
TGGGTTTGCCAAAAGAGTTAAAGGCAGAACTTGGACATTATGTGGAACTCCAGAGTATTT
GGCTCCAGAAATAATTCTCAGCAAGGGCTACAATAAGGCAGTGGATTGGTGGGCATTAGG
AGTGCTAATCTATGAAATGGCAGCTGGCTATCCCCCATTCTTTGCAGACCAACCAATTCA
GATTTATGAAAAGATTGTTTCTGGAAAGGTCCGATTCCCATCCCACTTCAGTTCAGATCT
CAAGGACCTTCTACGGAACCTGCTGCAGGTGGATTTGACCAAGAGATTTGGAAATCTAAA
GAATGGTGTCAGTGATATAAAAACTCACAAGTGGTTTGCCACGACAGATTGGATTGCTAT
TTACCAGAGGAAGGTTGAAGCTCCATTCATACCAAAGTTTAGAGGCTCTGGAGATACCAG
CAACTTTGATGACTATGAAGAAGAAGATATCCGTGTCTCTATAACAGAAAAATGTGCAAA
AGAATTTGGTGAATTTTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1287
Start on reference sequence 91
Mutations:
None
Discrepancy:
a162g; a316g,M106V
                                

5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PRKACB
Symbol Nomenclature : PRKACB
SYNONYM : PKACB
Designation : PKA C-beta|cAMP-dependent protein kinase catalytic beta subunit isoform 4ab|cAMP-dependent protein kinase catalytic subunit beta|protein kinase A catalytic subunit beta
Full Nomenclature : protein kinase, cAMP-dependent, catalytic, beta
GENEID : 5567
Locus Tag : RP11-82H13.1
GI : 190692146
GenBank Accession : EU832837
HGNC : 9381
MIM : 176892
Vega : OTTHUMG00000009975
Target GenBank: BX641026.1

 

Reference Sequence Alignment

  

HsCD00296752        -------------------------------------GTACAAAAAAGCAGGCTCCACCA
NM_182948.2         -----------------------------------------------------------A
XM_005259984.1      ATGTTCACCTTGGGATACCATGGGGCCGAGGAGTGGGCATCGGGCGAGACTTCCCTTGAA
                                                                               *

HsCD00296752        TGGCAGCTTATAGAGAACCACCTTGTAACCAGTATACAGGTACAACTACAGCTCTTCAGA
NM_182948.2         TGGCAGCTTATAGAGAACCACCTTGTAACCAGTATACAGGTACAACTACAGCTCTTCAGA
XM_005259984.1      CCAGCTCTGGAGCAATGCCCTTGCGATTGCCACGGAGAGGAACAGGGCCAGCTGGGGGCC
                      . . ** .:. *.:.**.    *::: *.. . * ***:***.  .*****    . .

HsCD00296752        AATTGGAAGGTTTTGCTAGCCGGTTATTTCATAGACACTCTAAAGGTACTGCACATGATC
NM_182948.2         AATTGGAAGGTTTTGCTAGCCGGTTATTTCATAGACACTCTAAAGGTACTGCACATGATC
XM_005259984.1      AAGGTGACAGCTGAGCCCCCCAAGGGCACCTGCTACCCGAGCCCCCGAGGCCTCCATCCC
                    **   **..* * :** . **..  . : *: . **.* . ...   *   *:*.: . *

HsCD00296752        AG----------------------------AAAACAGCTCTGGAAAATGACAGCCTTCAT
NM_182948.2         AG----------------------------AAAACAGCTCTGGAAAATGACAGCCTTCAT
XM_005259984.1      AGGCCCGGCCTCCCCAGAGAAGGGGCCAGAGGAACAGCCAAATCCCAAGGCTATAAATAC
                    **                            ..****** .:. ...*:*.*:. .:: * 

HsCD00296752        TTCTCTGAGCATACTGCCTTATGGGACAGATCAATGAAAGAGTTTCTAGCCAAAGCCAAA
NM_182948.2         TTCTCTGAACATACTGCCTTATGGGACAGATCAATGAAAGAGTTTCTAGCCAAAGCCAAA
XM_005259984.1      CTCGGCTGGCGTCGGCCCTGGCCATGGAATGTCGTGAAAGAATTCTTAGCCAAAGCCAAA
                     **    ..*.*.   *** .  . . *.:  ..*******.**  **************

HsCD00296752        GAAGACTTTTTGAAAAAATGGGAGAATCCAACTCAGAATAATGCCGGACTTGAAGATTTT
NM_182948.2         GAAGACTTTTTGAAAAAATGGGAGAATCCAACTCAGAATAATGCCGGACTTGAAGATTTT
XM_005259984.1      GAAGATTTTCTTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCACTTGGATCAGTTT
                    ***** *** * ***********.*.***..******* *.:*** .. * **: * ***

HsCD00296752        GAAAGGAAAAAAACCCTTGGAACAGGTTCATTTGGAAGAGTCGTGTTGGTAAAACACAAA
NM_182948.2         GAAAGGAAAAAAACCCTTGGAACAGGTTCATTTGGAAGAGTCATGTTGGTAAAACACAAA
XM_005259984.1      GAACGAATCAAGACCCTCGGCACGGGCTCCTTCGGGCGGGTGATGCTGGTGAAACACAAG
                    ***.*.*:.**.***** **.**.** **.** **..*.** .** ****.********.

HsCD00296752        GCCACTGAACAGTATTATGCCATGAAGATCTTAGATAAGCAGAAGGTTGTTAAACTGAAG
NM_182948.2         GCCACTGAACAGTATTATGCCATGAAGATCTTAGATAAGCAGAAGGTTGTTAAACTGAAG
XM_005259984.1      GAGACCGGGAACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTGGTGAAACTGAAA
                    *. ** *...*  * *************** *.** **.******** ** ********.

HsCD00296752        CAAATAGAGCATACTTTGAATGAGAAAAGAATATTACAGGCAGTGAATTTTCCTTTCCTT
NM_182948.2         CAAATAGAGCATACTTTGAATGAGAAAAGAATATTACAGGCAGTGAATTTTCCTTTCCTT
XM_005259984.1      CAGATCGAACACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTCAACTTTCCGTTCCTC
                    **.**.**.** **  *******.**..*.**. *.**.**:** ** ***** ***** 

HsCD00296752        GTTCGACTGGAGTATGCTTTTAAGGATAATTCTAATTTATACATGGTTATGGAATATGTC
NM_182948.2         GTTCGACTGGAGTATGCTTTTAAGGATAATTCTAATTTATACATGGTTATGGAATATGTC
XM_005259984.1      GTCAAACTCGAGTTCTCCTTCAAGGACAACTCAAACTTATACATGGTCATGGAGTACGTG
                    ** ..*** ****:  * ** ***** ** **:** *********** *****.** ** 

HsCD00296752        CCTGGGGGTGAGATGTTTTCACATCTAAGAAGAATTGGAAGGTTCAGTGAGCCCCATGCA
NM_182948.2         CCTGGGGGTGAAATGTTTTCACATCTAAGAAGAATTGGAAGGTTCAGTGAGCCCCATGCA
XM_005259984.1      CCCGGCGGGGAGATGTTCTCACACCTACGGCGGATCGGAAGGTTCAGTGAGCCCCATGCC
                    ** ** ** **.***** ***** ***.*..*.** ***********************.

HsCD00296752        CGGTTCTATGCAGCTCAGATAGTGCTAACATTCGAGTACCTCCATTCACTAGACCTCATC
NM_182948.2         CGGTTCTATGCAGCTCAGATAGTGCTAACATTCGAGTACCTCCATTCACTAGACCTCATC
XM_005259984.1      CGTTTCTACGCGGCCCAGATCGTCCTGACCTTTGAGTATCTGCACTCGCTGGATCTCATC
                    ** ***** **.** *****.** **.**.** ***** ** ** **.**.** ******

HsCD00296752        TACAGAGATCTAAAACCTGAAAATCTCTTAATTGACCATCAAGGCTATATCCAGGTCACA
NM_182948.2         TACAGAGATCTAAAACCTGAAAATCTCTTAATTGACCATCAAGGCTATATCCAGGTCACA
XM_005259984.1      TACAGGGACCTGAAGCCGGAGAATCTGCTCATTGACCAGCAGGGCTACATTCAGGTGACA
                    *****.** **.**.** **.*****  *.******** **.***** ** ***** ***

HsCD00296752        GACTTTGGGTTTGCCAAAAGAGTTAAAGGCAGAACTTGGACATTATGTGGAACTCCAGAG
NM_182948.2         GACTTTGGGTTTGCCAAAAGAGTTAAAGGCAGAACTTGGACATTATGTGGAACTCCAGAG
XM_005259984.1      GACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGCGGCACCCCTGAG
                    ***** ** ** *****..*.** **.***.*.********.**.** **.** **:***

HsCD00296752        TATTTGGCTCCAGAAATAATTCTCAGCAAGGGCTACAATAAGGCAGTGGATTGGTGGGCA
NM_182948.2         TATTTGGCTCCAGAAATAATTCTCAGCAAGGGCTACAATAAGGCAGTGGATTGGTGGGCA
XM_005259984.1      TACCTGGCCCCTGAGATTATCCTGAGCAAAGGCTACAACAAGGCCGTGGACTGGTGGGCC
                    **  **** **:**.**:** ** *****.******** *****.***** ********.

HsCD00296752        TTAGGAGTGCTAATCTATGAAATGGCAGCTGGCTATCCCCCATTCTTTGCAGACCAACCA
NM_182948.2         TTAGGAGTGCTAATCTATGAAATGGCAGCTGGCTATCCCCCATTCTTTGCAGACCAACCA
XM_005259984.1      CTGGGGGTTCTTATCTATGAAATGGCCGCTGGCTACCCGCCCTTCTTCGCAGACCAGCCC
                     *.**.** **:**************.******** ** **.***** ********.**.

HsCD00296752        ATTCAGATTTATGAAAAGATTGTTTCTGGAAAGGTCCGATTCCCATCCCACTTCAGTTCA
NM_182948.2         ATTCAGATTTATGAAAAGATTGTTTCTGGAAAGGTCCGATTCCCATCCCACTTCAGTTCA
XM_005259984.1      ATCCAGATCTATGAGAAGATCGTCTCTGGGAAGGTGCGCTTCCCTTCCCACTTCAGCTCT
                    ** ***** *****.***** ** *****.***** **.*****:*********** **:

HsCD00296752        GATCTCAAGGACCTTCTACGGAACCTGCTGCAGGTGGATTTGACCAAGAGATTTGGAAAT
NM_182948.2         GATCTCAAGGACCTTCTACGGAACCTGCTGCAGGTGGATTTGACCAAGAGATTTGGAAAT
XM_005259984.1      GACTTGAAGGACCTGCTGCGGAACCTCCTGCAGGTAGATCTCACCAAGCGCTTTGGGAAC
                    **  * ******** **.******** ********.*** * ******.*.*****.** 

HsCD00296752        CTAAAGAATGGTGTCAGTGATATAAAAACTCACAAGTGGTTTGCCACGACAGATTGGATT
NM_182948.2         CTAAAGAATGGTGTCAGTGATATAAAAACTCACAAGTGGTTTGCCACGACAGATTGGATT
XM_005259984.1      CTCAAGAATGGGGTCAACGATATCAAGAACCACAAGTGGTTTGCCACAACTGACTGGATT
                    **.******** ****. *****.**.*. *****************.**:** ******

HsCD00296752        GCTATTTACCAGAGGAAGGTTGAAGCTCCATTCATACCAAAGTTTAGAGGCTCTGGAGAT
NM_182948.2         GCTATTTACCAGAGGAAGGTTGAAGCTCCATTCATACCAAAGTTTAGAGGCTCTGGAGAT
XM_005259984.1      GCCATCTACCAGAGGAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGAT
                    ** ** ************** ********.****************.**** ****.***

HsCD00296752        ACCAGCAACTTTGATGACTATGAAGAAGAAGATATCCGTGTCTCTATAACAGAAAAATGT
NM_182948.2         ACCAGCAACTTTGATGACTATGAAGAAGAAGATATCCGTGTCTCTATAACAGAAAAATGT
XM_005259984.1      ACGAGTAACTTTGACGACTATGAGGAAGAAGAAATCCGGGTCTCCATCAATGAGAAGTGT
                    ** ** ******** ********.********:***** ***** **.*.:**.**.***

HsCD00296752        GCAAAAGAATTTGGTGAATTTTGAATCCACCCAGCTTTCTTGTAC
NM_182948.2         GCAAAAGAATTTGGTGAATTTTAA---------------------
XM_005259984.1      GGCAAGGAGTTTTCTGAGTTTTAG---------------------
                    * .**.**.***  ***.****..                     

Pathways

NCI : Alpha4 beta1 integrin signaling events
NCI : GMCSF-mediated signaling events
NCI : Glucocorticoid receptor regulatory network
NCI : IL3-mediated signaling events
NCI : p73 transcription factor network
Panther : 5HT1 type receptor mediated signaling pathway
Panther : Beta1 adrenergic receptor signaling pathway
Panther : Beta2 adrenergic receptor signaling pathway
Panther : Dopamine receptor mediated signaling pathway
Panther : Endothelin signaling pathway
Panther : Enkephalin release
Panther : GABA-B receptor II signaling
Panther : Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Panther : Heterotrimeric G-protein signaling pathway-rod outer segment phototransduction
Panther : Histamine H2 receptor mediated signaling pathway
Panther : Inflammation mediated by chemokine and cytokine signaling pathway
Panther : Metabotropic glutamate receptor group I pathway
Panther : Metabotropic glutamate receptor group II pathway
Panther : Metabotropic glutamate receptor group III pathway
Panther : Muscarinic acetylcholine receptor 2 and 4 signaling pathway
Reactome : Activation of NMDA receptor upon glutamate binding and postsynaptic events
Reactome : Adaptive Immune System
Reactome : Aquaporin-mediated transport
Reactome : CREB phosphorylation through the activation of Adenylate Cyclase
Reactome : Ca-dependent events
Reactome : CaM pathway
Reactome : Calmodulin induced events
Reactome : Cytokine Signaling in Immune system
Reactome : DAG and IP3 signaling
Reactome : DAP12 interactions
Reactome : DAP12 signaling
Reactome : DARPP-32 events
Reactome : Disease
Reactome : Downstream signal transduction
Reactome : Downstream signaling of activated FGFR
Reactome : EGFR interacts with phospholipase C-gamma
Reactome : Factors involved in megakaryocyte development and platelet production
Reactome : G-protein mediated events
Reactome : Glucagon signaling in metabolic regulation
Reactome : Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
Reactome : Gluconeogenesis
Reactome : Glucose metabolism
Reactome : Glycogen storage diseases
Reactome : Hemostasis
Reactome : Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Integration of energy metabolism
Reactome : Interleukin-3, 5 and GM-CSF signaling
Reactome : Lipid digestion, mobilization, and transport
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Metabolism of lipids and lipoproteins
Reactome : Myoclonic epilepsy of Lafora
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Opioid Signalling
Reactome : PKA activation
Reactome : PKA activation in glucagon signalling
Reactome : PKA-mediated phosphorylation of CREB
Reactome : PKA-mediated phosphorylation of key metabolic factors
Reactome : PLC beta mediated events
Reactome : PLC-gamma1 signalling
Reactome : PLCG1 events in ERBB2 signaling
Reactome : Phospholipase C-mediated cascade
Reactome : Post NMDA receptor activation events
Reactome : Rap1 signalling
Reactome : Regulation of insulin secretion
Reactome : Regulation of water balance by renal Aquaporins
Reactome : Signal Transduction
Reactome : Signaling by EGFR
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by FGFR
Reactome : Signaling by FGFR in disease
Reactome : Signaling by GPCR
Reactome : Signaling by Interleukins
Reactome : Signaling by PDGF
Reactome : Signalling by NGF
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses
WikiPathway : AMPK signaling
WikiPathway : Calcium Regulation in the Cardiac Cell
WikiPathway : G Protein Signaling Pathways
WikiPathway : Myometrial Relaxation and Contraction Pathways
WikiPathway : miRs in Muscle Cell Differentiation

Clone

Cloning Information : 483094
HIP Master Clone ID : 400822
IMAGE ID : 100067866
Original Clone ID : FLH483094.01X

Organism


TAX_ID : 9606
Species Specific ID: 5567

Genome

Chromosome : 1
Map Location : 1p31.1
Ensembl : ENSG00000142875

Reagents

Labome : PRKACB-antibody