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PNMT (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PNMT
Gene Name:  phenylethanolamine N-methyltransferase
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH483195.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296853

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 849nts         Open reading frame : 23 to 849
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGAGCGGCGCAGACCGTAGCCCCAATGCGGGCGCAGC
CCCTGACTCGGCCCCGGGCCAGGCGGCGGTGGCTTCGGCCTACCAGCGCTTCGAGCCGCG
CGCCTACCTCCGCAACAACTACGCGCCCCCTCGCGGGGACCTGTGCAACCCGAACGGCGT
CGGGCCGTGGAAGCTGCGCTGCTTGGCGCAGACCTTCGCCACCGGTGAAGTGTCCGGACG
CACCCTCATCGACATTGGTTCAGGCCCCACCGTGTACCAGCTGCTCAGTGCCTGCAGCCA
CTTTGAGGACATCACCATGACAGATTTCCTGGAGGTCAACCGCCAGGAGCTGGGGCGCTG
GCTGCAGGAGGAGCCGGGGGCCTTCAACTGGAGCATGTACAGCCAACATGCCTGCCTCAT
TGAGGGCAAGGGGGAATGCTGGCAGGATAAGGAGCGCCAGCTGCGAGCCAGGGTGAAACG
GGTCCTGCCCATCGACGTGCACCAGCCCCAGCCCCTGGGTGCTGGGAGCCCAGCTCCCCT
GCCTGCTGACGCCCTGGTCTCTGCCTTCTGCTTGGAGGCTGTGAGCCCAGATCTTGCCAG
CTTTCAGCGGGCCCTGGACCACATCACCACGCTGCTGAGGCCTGGGGGGCACCTCCTCCT
CATCGGGGCCCTGGAGGAGTCGTGGTACCTGGCTGGGGAGGCCAGGCTGACGGTGGTGCC
AGTGTCTGAGGAGGAGGTGAGGGAGGCCCTGGTGCGTAGTGGCTACAAGGTCCGGGACCT
CCGCACCTATATCATGCCTGCCCACCTTCAGACAGGCGTAGATGATGTCAAGGGCGTCTT
CTTCGCCTGGGCTCAGAAGGTTGGGCTGTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1071
Start on reference sequence 223
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PNMT
Symbol Nomenclature : PNMT
SYNONYM : PENT; PNMTase
Designation : noradrenaline N-methyltransferase|phenylethanolamine N-methylase
Full Nomenclature : phenylethanolamine N-methyltransferase
GENEID : 5409
GI : 190691378
GenBank Accession : EU832114
HGNC : 9160
MIM : 171190
Target GenBank: BC037246.1

 

Reference Sequence Alignment

  

HsCD00296853      GTACAAAAAAGCAGGCTCCACCATGAGCGGCGCAGACCGTAGCCCCAATGCGGGCGCAGC
NM_002686.4       ----------------------ATGAGCGGCGCAGACCGTAGCCCCAATGCGGGCGCAGC
                                        **************************************

HsCD00296853      CCCTGACTCGGCCCCGGGCCAGGCGGCGGTGGCTTCGGCCTACCAGCGCTTCGAGCCGCG
NM_002686.4       CCCTGACTCGGCCCCGGGCCAGGCGGCGGTGGCTTCGGCCTACCAGCGCTTCGAGCCGCG
                  ************************************************************

HsCD00296853      CGCCTACCTCCGCAACAACTACGCGCCCCCTCGCGGGGACCTGTGCAACCCGAACGGCGT
NM_002686.4       CGCCTACCTCCGCAACAACTACGCGCCCCCTCGCGGGGACCTGTGCAACCCGAACGGCGT
                  ************************************************************

HsCD00296853      CGGGCCGTGGAAGCTGCGCTGCTTGGCGCAGACCTTCGCCACCGGTGAAGTGTCCGGACG
NM_002686.4       CGGGCCGTGGAAGCTGCGCTGCTTGGCGCAGACCTTCGCCACCGGTGAAGTGTCCGGACG
                  ************************************************************

HsCD00296853      CACCCTCATCGACATTGGTTCAGGCCCCACCGTGTACCAGCTGCTCAGTGCCTGCAGCCA
NM_002686.4       CACCCTCATCGACATTGGTTCAGGCCCCACCGTGTACCAGCTGCTCAGTGCCTGCAGCCA
                  ************************************************************

HsCD00296853      CTTTGAGGACATCACCATGACAGATTTCCTGGAGGTCAACCGCCAGGAGCTGGGGCGCTG
NM_002686.4       CTTTGAGGACATCACCATGACAGATTTCCTGGAGGTCAACCGCCAGGAGCTGGGGCGCTG
                  ************************************************************

HsCD00296853      GCTGCAGGAGGAGCCGGGGGCCTTCAACTGGAGCATGTACAGCCAACATGCCTGCCTCAT
NM_002686.4       GCTGCAGGAGGAGCCGGGGGCCTTCAACTGGAGCATGTACAGCCAACATGCCTGCCTCAT
                  ************************************************************

HsCD00296853      TGAGGGCAAGGGGGAATGCTGGCAGGATAAGGAGCGCCAGCTGCGAGCCAGGGTGAAACG
NM_002686.4       TGAGGGCAAGGGGGAATGCTGGCAGGATAAGGAGCGCCAGCTGCGAGCCAGGGTGAAACG
                  ************************************************************

HsCD00296853      GGTCCTGCCCATCGACGTGCACCAGCCCCAGCCCCTGGGTGCTGGGAGCCCAGCTCCCCT
NM_002686.4       GGTCCTGCCCATCGACGTGCACCAGCCCCAGCCCCTGGGTGCTGGGAGCCCAGCTCCCCT
                  ************************************************************

HsCD00296853      GCCTGCTGACGCCCTGGTCTCTGCCTTCTGCTTGGAGGCTGTGAGCCCAGATCTTGCCAG
NM_002686.4       GCCTGCTGACGCCCTGGTCTCTGCCTTCTGCTTGGAGGCTGTGAGCCCAGATCTTGCCAG
                  ************************************************************

HsCD00296853      CTTTCAGCGGGCCCTGGACCACATCACCACGCTGCTGAGGCCTGGGGGGCACCTCCTCCT
NM_002686.4       CTTTCAGCGGGCCCTGGACCACATCACCACGCTGCTGAGGCCTGGGGGGCACCTCCTCCT
                  ************************************************************

HsCD00296853      CATCGGGGCCCTGGAGGAGTCGTGGTACCTGGCTGGGGAGGCCAGGCTGACGGTGGTGCC
NM_002686.4       CATCGGGGCCCTGGAGGAGTCGTGGTACCTGGCTGGGGAGGCCAGGCTGACGGTGGTGCC
                  ************************************************************

HsCD00296853      AGTGTCTGAGGAGGAGGTGAGGGAGGCCCTGGTGCGTAGTGGCTACAAGGTCCGGGACCT
NM_002686.4       AGTGTCTGAGGAGGAGGTGAGGGAGGCCCTGGTGCGTAGTGGCTACAAGGTCCGGGACCT
                  ************************************************************

HsCD00296853      CCGCACCTATATCATGCCTGCCCACCTTCAGACAGGCGTAGATGATGTCAAGGGCGTCTT
NM_002686.4       CCGCACCTATATCATGCCTGCCCACCTTCAGACAGGCGTAGATGATGTCAAGGGCGTCTT
                  ************************************************************

HsCD00296853      CTTCGCCTGGGCTCAGAAGGTTGGGCTGTGAATCCACCCAGCTTTCTTGTAC
NM_002686.4       CTTCGCCTGGGCTCAGAAGGTTGGGCTGTGA---------------------
                  *******************************                     

Pathways

Panther : Adrenaline and noradrenaline biosynthesis
Reactome : Amine-derived hormones
Reactome : Catecholamine biosynthesis
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
WikiPathway : Biogenic Amine Synthesis
WikiPathway : Methylation Pathways

Protein

UniProt : P11086
HPRD : 07176

Clone

Cloning Information : 483195
HIP Master Clone ID : 400923
IMAGE ID : 100067143
Original Clone ID : FLH483195.01X

Organism


TAX_ID : 9606
Species Specific ID: 5409

Genome

Chromosome : 17
Map Location : 17q

Reagents

Labome : PNMT-antibody