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ACTRT1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTRT1
Gene Name:  actin-related protein T1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH483213.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296871

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1131nts        
Open reading frame : 23 to 1153
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAAT
TTTTGACAATGGTTCAGGACTCTGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCA
TGTCATCAGCTCCGTCTTGGGACATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCA
GAAGTACTTCGTGGGGCAAGAAGCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCC
CATTGAGCGTGGACTGGTAACAGGATGGGATGACATGGAGAAACTCTGGAAACATCTCTT
TGAGCGGGAGCTTGGAGTAAAACCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTT
GAATCCTAGGGAAATTCGAGAAAAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCC
TGGTTTCTACCTGTCTAATCATGCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGG
CCTGGTGGTGGACAGTGGAGATGGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTC
CCTGCCTCACGCAGTCACCAAACTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCAC
CCGGCTCCTCTTTGCTAGCGGGTTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAA
TAACATCAAAGAGAAGTTGTGCTACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAG
CCGGGGAGAGGTCCTGGGAGCATACAGACTGCCAGATGGACATGTCATCCACTTTGGGGA
TGAGCTGTACCAAGTGCCCGAGGTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCC
AGGACTCTCAAAAATGGTCTCCAGCAGCATCATGAAGTGTGACACTGACATCCAGAATAA
ACTTTATGCAGACATTGTACTCTCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAG
GCTCATGAAGGAAGTGGAACAGCTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTC
TCCTGATAGATGCTTCTCTGCATGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTT
CAAGCAGATGTGGGTCACCTCGGCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAG
AAGGTGCTTTTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1326
Start on reference sequence 196
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site U19 primer 517 535
primer site Att P2 Primer 3026 3043
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTRT1
Symbol Nomenclature : ACTRT1
SYNONYM : AIP1; ARIP1; ARPT1; HSD27
Full Nomenclature : actin-related protein T1
GENEID : 139741
Locus Tag : RP1-203C2.1
GI : 190691500
GenBank Accession : EU832175
HGNC : 24027
MIM : 300487
Vega : OTTHUMG00000022359
Target GenBank: BC014597.1

 

Reference Sequence Alignment

  

HsCD00296871      GTACAAAAAAGCAGGCTCCACCATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAAT
NM_138289.3       ----------------------ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAAT
                                        **************************************

HsCD00296871      TTTTGACAATGGTTCAGGACTCTGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCA
NM_138289.3       TTTTGACAATGGTTCAGGACTCTGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCA
                  ************************************************************

HsCD00296871      TGTCATCAGCTCCGTCTTGGGACATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCA
NM_138289.3       TGTCATCAGCTCCGTCTTGGGACATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCA
                  ************************************************************

HsCD00296871      GAAGTACTTCGTGGGGCAAGAAGCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCC
NM_138289.3       GAAGTACTTCGTGGGGCAAGAAGCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCC
                  ************************************************************

HsCD00296871      CATTGAGCGTGGACTGGTAACAGGATGGGATGACATGGAGAAACTCTGGAAACATCTCTT
NM_138289.3       CATTGAGCGTGGACTGGTAACAGGATGGGATGACATGGAGAAACTCTGGAAACATCTCTT
                  ************************************************************

HsCD00296871      TGAGCGGGAGCTTGGAGTAAAACCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTT
NM_138289.3       TGAGCGGGAGCTTGGAGTAAAACCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTT
                  ************************************************************

HsCD00296871      GAATCCTAGGGAAATTCGAGAAAAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCC
NM_138289.3       GAATCCTAGGGAAATTCGAGAAAAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCC
                  ************************************************************

HsCD00296871      TGGTTTCTACCTGTCTAATCATGCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGG
NM_138289.3       TGGTTTCTACCTGTCTAATCATGCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGG
                  ************************************************************

HsCD00296871      CCTGGTGGTGGACAGTGGAGATGGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTC
NM_138289.3       CCTGGTGGTGGACAGTGGAGATGGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTC
                  ************************************************************

HsCD00296871      CCTGCCTCACGCAGTCACCAAACTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCAC
NM_138289.3       CCTGCCTCACGCAGTCACCAAACTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCAC
                  ************************************************************

HsCD00296871      CCGGCTCCTCTTTGCTAGCGGGTTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAA
NM_138289.3       CCGGCTCCTCTTTGCTAGCGGGTTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAA
                  ************************************************************

HsCD00296871      TAACATCAAAGAGAAGTTGTGCTACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAG
NM_138289.3       TAACATCAAAGAGAAGTTGTGCTACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAG
                  ************************************************************

HsCD00296871      CCGGGGAGAGGTCCTGGGAGCATACAGACTGCCAGATGGACATGTCATCCACTTTGGGGA
NM_138289.3       CCGGGGAGAGGTCCTGGGAGCATACAGACTGCCAGATGGACATGTCATCCACTTTGGGGA
                  ************************************************************

HsCD00296871      TGAGCTGTACCAAGTGCCCGAGGTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCC
NM_138289.3       TGAGCTGTACCAAGTGCCCGAGGTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCC
                  ************************************************************

HsCD00296871      AGGACTCTCAAAAATGGTCTCCAGCAGCATCATGAAGTGTGACACTGACATCCAGAATAA
NM_138289.3       AGGACTCTCAAAAATGGTCTCCAGCAGCATCATGAAGTGTGACACTGACATCCAGAATAA
                  ************************************************************

HsCD00296871      ACTTTATGCAGACATTGTACTCTCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAG
NM_138289.3       ACTTTATGCAGACATTGTACTCTCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAG
                  ************************************************************

HsCD00296871      GCTCATGAAGGAAGTGGAACAGCTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTC
NM_138289.3       GCTCATGAAGGAAGTGGAACAGCTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTC
                  ************************************************************

HsCD00296871      TCCTGATAGATGCTTCTCTGCATGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTT
NM_138289.3       TCCTGATAGATGCTTCTCTGCATGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTT
                  ************************************************************

HsCD00296871      CAAGCAGATGTGGGTCACCTCGGCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAG
NM_138289.3       CAAGCAGATGTGGGTCACCTCGGCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAG
                  ************************************************************

HsCD00296871      AAGGTGCTTTTGAATCCACCCAGCTTTCTTGTAC
NM_138289.3       AAGGTGCTTTTAA---------------------
                  ***********.*                     

Protein

SMART domain : ACTIN : Actin
UniProt : Q8TDG2
HPRD : 06439

Clone

Cloning Information : 483213
HIP Master Clone ID : 400941
IMAGE ID : 100067204
Original Clone ID : FLH483213.01X

Organism


TAX_ID : 9606
Species Specific ID: 139741

Genome

Chromosome : X
Map Location : Xq25
Ensembl : ENSG00000123165

Reagents

Labome : ACTRT1-antibody