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FGF16 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF16
Gene Name:  fibroblast growth factor 16
Sequence             
Original Clone ID: FLH270912.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00297371

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 624nts         Open reading frame : 35 to 658
Reference Sequence Alignment

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCAGAGGTGGGGGGCGTCTTCGC
CTCCTTGGACTGGGATCTACACGGCTTCTCCTCGTCTCTGGGGAACGTGCCCTTAGCTGA
CTCCCCAGGTTTCCTGAACGAGCGCCTGGGCCAAATCGAGGGGAAGCTGCAGCGTGGCTC
ACCCACAGACTTCGCCCACCTGAAGGGGATCCTGCGGCGCCGCCAGCTCTACTGCCGCAC
CGGCTTCCACCTGGAGATCTTCCCCAACGGCACGGTGCACGGGACCCGCCACGACCACAG
CCGCTTCGGAATCCTGGAGTTTATCAGCCTGGCTGTGGGGCTGATCAGCATCCGGGGAGT
GGACTCTGGCCTGTACCTAGGAATGAATGAGCGAGGAGAACTCTATGGGTCGAAGAAACT
CACACGTGAATGTGTTTTCCGGGAACAGTTTGAAGAAAACTGGTACAACACCTATGCCTC
AACCTTGTACAAACATTCGGACTCAGAGAGACAGTATTACGTGGCCCTGAACAAAGATGG
CTCACCCCGGGAGGGATACAGGACTAAACGACACCAGAAATTCACTCACTTTTTACCCAG
GCCTGTAGATCCTTCTAAGTTGCCCTCCATGTCCAGAGACCTCTTTCACTATAGGTAAGG
CCTCATGGGCCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 624
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF16
Symbol Nomenclature : FGF16
SYNONYM : FGF-16; MF4
Designation : metacarpal 4-5 fusion
Full Nomenclature : fibroblast growth factor 16
GENEID : 8823
Locus Tag : XX-FW80732C2.1
GI : 157169759
GenBank Accession : BC153196
HGNC : 3672
MIM : 300827
Target GenBank: NM_003868.1

 

Reference Sequence Alignment

  

HsCD00297371      GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCAGAGGTGGGGGGCGTCTTCGC
NM_003868.1       ----------------------------------ATGGCAGAGGTGGGGGGCGTCTTCGC
                                                    **************************

HsCD00297371      CTCCTTGGACTGGGATCTACACGGCTTCTCCTCGTCTCTGGGGAACGTGCCCTTAGCTGA
NM_003868.1       CTCCTTGGACTGGGATCTACACGGCTTCTCCTCGTCTCTGGGGAACGTGCCCTTAGCTGA
                  ************************************************************

HsCD00297371      CTCCCCAGGTTTCCTGAACGAGCGCCTGGGCCAAATCGAGGGGAAGCTGCAGCGTGGCTC
NM_003868.1       CTCCCCAGGTTTCCTGAACGAGCGCCTGGGCCAAATCGAGGGGAAGCTGCAGCGTGGCTC
                  ************************************************************

HsCD00297371      ACCCACAGACTTCGCCCACCTGAAGGGGATCCTGCGGCGCCGCCAGCTCTACTGCCGCAC
NM_003868.1       ACCCACAGACTTCGCCCACCTGAAGGGGATCCTGCGGCGCCGCCAGCTCTACTGCCGCAC
                  ************************************************************

HsCD00297371      CGGCTTCCACCTGGAGATCTTCCCCAACGGCACGGTGCACGGGACCCGCCACGACCACAG
NM_003868.1       CGGCTTCCACCTGGAGATCTTCCCCAACGGCACGGTGCACGGGACCCGCCACGACCACAG
                  ************************************************************

HsCD00297371      CCGCTTCGGAATCCTGGAGTTTATCAGCCTGGCTGTGGGGCTGATCAGCATCCGGGGAGT
NM_003868.1       CCGCTTCGGAATCCTGGAGTTTATCAGCCTGGCTGTGGGGCTGATCAGCATCCGGGGAGT
                  ************************************************************

HsCD00297371      GGACTCTGGCCTGTACCTAGGAATGAATGAGCGAGGAGAACTCTATGGGTCGAAGAAACT
NM_003868.1       GGACTCTGGCCTGTACCTAGGAATGAATGAGCGAGGAGAACTCTATGGGTCGAAGAAACT
                  ************************************************************

HsCD00297371      CACACGTGAATGTGTTTTCCGGGAACAGTTTGAAGAAAACTGGTACAACACCTATGCCTC
NM_003868.1       CACACGTGAATGTGTTTTCCGGGAACAGTTTGAAGAAAACTGGTACAACACCTATGCCTC
                  ************************************************************

HsCD00297371      AACCTTGTACAAACATTCGGACTCAGAGAGACAGTATTACGTGGCCCTGAACAAAGATGG
NM_003868.1       AACCTTGTACAAACATTCGGACTCAGAGAGACAGTATTACGTGGCCCTGAACAAAGATGG
                  ************************************************************

HsCD00297371      CTCACCCCGGGAGGGATACAGGACTAAACGACACCAGAAATTCACTCACTTTTTACCCAG
NM_003868.1       CTCACCCCGGGAGGGATACAGGACTAAACGACACCAGAAATTCACTCACTTTTTACCCAG
                  ************************************************************

HsCD00297371      GCCTGTAGATCCTTCTAAGTTGCCCTCCATGTCCAGAGACCTCTTTCACTATAGGTAAGG
NM_003868.1       GCCTGTAGATCCTTCTAAGTTGCCCTCCATGTCCAGAGACCTCTTTCACTATAGGTAA--
                  **********************************************************  

HsCD00297371      CCTCATGGGCCCAGCTTTCTTGTAC
NM_003868.1       -------------------------
                                           

Pathways

Reactome : Activated point mutants of FGFR2
Reactome : Adaptive Immune System
Reactome : Constitutive PI3K/AKT Signaling in Cancer
Reactome : DAP12 interactions
Reactome : DAP12 signaling
Reactome : Disease
Reactome : Downstream signal transduction
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : Downstream signaling of activated FGFR
Reactome : FGFR ligand binding and activation
Reactome : FGFR2 ligand binding and activation
Reactome : FGFR2c ligand binding and activation
Reactome : FGFR3 ligand binding and activation
Reactome : FGFR3c ligand binding and activation
Reactome : FGFR4 ligand binding and activation
Reactome : FRS2-mediated cascade
Reactome : Fc epsilon receptor (FCERI) signaling
Reactome : GAB1 signalosome
Reactome : IGF1R signaling cascade
Reactome : IRS-mediated signalling
Reactome : IRS-related events
Reactome : IRS-related events triggered by IGF1R
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Insulin receptor signalling cascade
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : Negative regulation of FGFR signaling
Reactome : PI-3K cascade
Reactome : PI3K Cascade
Reactome : PI3K events in ERBB2 signaling
Reactome : PI3K events in ERBB4 signaling
Reactome : PI3K/AKT Signaling in Cancer
Reactome : PI3K/AKT activation
Reactome : PIP3 activates AKT signaling
Reactome : Phospholipase C-mediated cascade
Reactome : Role of LAT2/NTAL/LAB on calcium mobilization
Reactome : SHC-mediated cascade
Reactome : Signal Transduction
Reactome : Signaling by EGFR
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by ERBB4
Reactome : Signaling by FGFR
Reactome : Signaling by FGFR in disease
Reactome : Signaling by FGFR mutants
Reactome : Signaling by FGFR2 mutants
Reactome : Signaling by FGFR3 mutants
Reactome : Signaling by Insulin receptor
Reactome : Signaling by PDGF
Reactome : Signaling by SCF-KIT
Reactome : Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome : Signaling by activated point mutants of FGFR3
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Signalling by NGF

Protein

SMART domain : FGF : Acidic and basic fibroblast growth factor family.
UniProt : O43320
HPRD : 04764

Clone

Cloning Information : 270912
HIP Master Clone ID : 202201
IMAGE ID : 100016568
Original Clone ID : FLH270912.01X

Organism


TAX_ID : 9606
Species Specific ID: 8823

Genome

Chromosome : X
Map Location : Xq13

Reagents

Labome : FGF16-antibody