Gene:
Gene Symbol:
Mllt4
Gene Name:
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
Original Clone ID:
FLH481792.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297473
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5463nts
Open reading frame: 35 to 5497
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCGGCGGGCGGCCGCGACGAAGA
GCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACCGGCTGGACCTGTTCGA
GATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTTTCAAGA
TAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGAGTCTCTAGCACAGCCACCACTCA
AGATGTGATTGAAACACTGGCAGAGAAGTTCCGCCCTGACATGCGCATGCTCTCCTCTCC
CAAGTACTCCCTCTATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAA
ACCTCTAGTTGTACAGTTGAATTGGAACAAAGATGATCGAGAGGGTCGATTCGTCCTTAA
GAACGAGAATGACGCCATTCCTGCCAAGAAGGCTCAGAGTAATGGACCTGAAAAGCAGGA
GAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTCTCAAAGAAAGAAAAGAAGGAAAA
AAAGAAGAAAGAGAAGGAGGCATTGAGACAAGCCTCTGATAAGGAAGAAAGGCCTTCTCA
AGGGGATGACAGTGAGAACTCACGACTGGCTGCTGAAGTTTACAAAGACATGCCTGAGAC
CAGCTTTACTAGGACCATTTCTAACCCTGAAGTTGTCATGAAACGGCGGCGGCAGCAAAA
ACTGGAGAAGAGAATGCAAGAGTTTCGGAGCTCAGATGGGCGGCCTGACTCAGGTGGAAC
ACTGAGGATATATGCAGATAGTTTAAAACCAAATATTCCATACAAGACAATCCTGCTGTC
TACTACCGATACCGCAGACTTTGCTGTAGCCGAATCTTTGGAGAAGTATGGTCTGGAAAA
AGAGAATCCCAAGGACTACTGTATTGCCCGGGTTATGCTTCCTCCTGGAGCCCAGCATTC
TGATGAGAGAGGTGCTAAGGAAATAATTCTTGACGATGATGAATGTCCTTTACAAATCTT
CAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGA
CTACATCCCAAAGAAAATGAAGAAACATGTTGAAGGGAAGTCGTTGAAGGGGAAAGACAG
AGCTGACGGGTCTGGCTACGGCTCCGCTCTTCCTCCCGAGAAGCTGCCCTACTTAGTAGA
GCTAAGCCCAGGGAGAAGGAATCACTTTGCCTACTACAGCTATCACACTTACGAAGATGG
CTCTGACTCCAGAGACAAGCCAAAGTTATACCGCCTTCAATTAAGTGTGACTGAAGTTGG
GACAGAGAAATTTGATGACAATTCTATCCAGCTGTTTGGCCCCGGAATTCAGCCCCATCA
CTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGATGCAGA
GACCTACGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTGCAGAGTGGCATGAGACT
GCAGTTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTC
CAAGAGATCTGTGGATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCTGT
TCAGGAGACAACCTTTGAATTGGGAGGAGATGTCCACAGTGGGACAGCATTGCCGGCAAG
CAGGAGCACTACTAGACTGGACAGTGACAGGGTGTCCTCTGCCTCTAGCACAGCTGAGCG
AGGGATGGTGAAGCCGATGATCCGACTGGACCAGGAGCAGGAATATCGACGACGAGAAAA
CAGGACTCAGGATGCTACTGGGCCTGAACTGATACTGCCTGCCAGCATTGAATTCAGGGA
AAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGTTCTACAGTTCA
TTTTAAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCA
GCACAGGCCCGACATCAGTCCTACAGAGCGTACACATAAGGCCATTGCTGTTGTCAACAA
GATGGTGAGCATGATGGAAGGGGTCATCCAGAAACAGAAGAACATTGCAGGAGCACTTGC
TTTTTGGATGGCAAATGCATCTGAGCTTCTCAACTTCATCAAGCAGGACCGAGACCTTAG
TCGGATCACGCTGGATGCCCAGGATGTCCTAGCACACTTGGTTCAGATGGCATTTAAGTA
CTTGGTTCATTGCCTTCAGTCAGAACTTAATAACTACATGCCAGCCTTTCTGGATGATCC
TGAGGAGAACAGTCTGCAAAGACCAAAAATAGATGATGTGCTGCACACACTCACAGGAGC
CATGTCCTTGCTGCGGCGCTGCAGAGTCAATGCTGCCTTGACCATCCAGCTCTTCTCTCA
GCTTTTCCACTTCATCAATATGTGGCTGTTCAACAGACTGGTGACTGACCCAGATTCAGG
CCTGTGCTCCCACTACTGGGGTGCCATTATCCGCCAGCAGCTGGGGCATATTGAAGCGTG
GGCAGAGAAGCAGGGGCTAGAGCTGGCAGCTGACTGTCACTTGAGCAGAATTGTACAGGC
TACAACATTGCTTACGATGGATAAGTATGTACCTGATGACATTCCAAATATAAACAGCAC
ATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGCCTTGTTACAAAATTACCACTGTGCACC
CGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTTGTGGCTGTTGCTGAGAACAC
AGCTGATGAGCTCGCCCGCAGTGATGGCAGAGACGTGCAGCTGGAGGAAGACCCAGACCT
ACAGCTGCCTTTTCTTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCC
AAATGGTTTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGTAGATTGGTTCC
TCACACACGTTCACCAGGTACCTGGACAATATATTTTGAAGGAGCAGATTATGAAAGTCA
CCTTATGCGTGAGAACGCAGAACTGGCCCAGCCTCTGAGGAAGGAGCCTGAGATAATCAC
TGTGACTCTGAAGAAGCAGAATGGAATGGGCCTCAGCATCGTTGCAGCCAAGGGTGCTGG
CCAGGATAAACTGGGAATCTACGTTAAGTCGGTTGTCAAAGGAGGTGCTGCAGATGTGGA
TGGACGATTAGCTGCTGGTGACCAACTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACT
TTCTCAGGAAAGGGCAGCAGAACTCATGACAAGAACCAGTTCCGTGGTTACACTGGAAGT
TGCAAAGCAAGGTGCCATCTATCACGGCCTGGCCACCCTCCTTAACCAGCCGTCCCCCAT
GATGCAAAGAATTTCAGATCGTCGTGGCTCAGGTAAACCCAGACCAAAGAGTGAAGGTTT
TGAGCTCTATAATAATTCAGCTCAAAATGGCTCCCCAGAGAGTCCTCAGATGCCTTGGAC
AGAGTACAGTGAGCCAAAGAAACTGCCTGGGGATGACAGGCTGATGAAAAACAGAGCGGA
CCACCGTTCCAGCCCCAATGTGGCAAATCAGCCTCCTAGCCCTGGAGGGAAGGGTCCATA
TACATCTGGAACAGCAGCTAAGATAACATCTGTCTCCACTGGAAACCTCTGCACTGAGGA
GCAGTCCCCTCCACCCAGACCTGAAGCCTATCCTATCCCTACTCAGACATACACCAGAGA
GTATTTTACCTTTCCAGCTTCAAAGTCGCAGGATCGGATGGCTCCTCCTCAGAGCCAGTG
GCCAAATTATGAGGAAAAACCACATGTGCACACAGAAAGTAATCATTCCAGTATTGCAAT
CCAGCGTGTTACCCGTTCCCAGGAAGAGCTTCGGGAAGAAAAGGTTTACCAACTTGAGCG
GCATCGAGTAGAGGCAGGCATGGATCGCAAGTGTGACAGTGATATGTGGATCAATCAGAG
CTCCTCAGTGGAATCCAGCACATCCAGCCAGGAACACCTGAACCATTCTTCCAAGTCAGT
TACCCCTGCTTCCACTCTGACCAAGAGTGGTCCTGGCCGTTGGAAAACACCAGCAGCGGT
GCTGCCCACCCCTGTGGCTGTCTCTCAGCCCATTCGAACAGACCTCCCTCCACCACCACC
TCCACCGCCGGTCCACTACACCAGTGAGTTTGATGGTATTCCAATGGATTTGCCCCTCCC
ACCTCCTCCTGCCAACCAGGCAGGACCCCAGTCTGCTCAGGTGGCTGCTGCAGAGTGGAA
AAAGAGAGAGGAGCACCAGAGGTGGTATGAGAAAGAGAAGGCCCGCCTGGAGGAAGAACG
AGAGAGAAAGCGCAGGGAGCAGGAGCGGAAGTTGGGGCAGATGCGCTCTCAGACCCTGAA
CCCTGCTTCCTTCTCACCTTTGGCCACACAGGCCAAGCCAGAAAAGCCTTCCACACTGCA
GAGGCCCCAAGAAACAGTCATTCGGGAGCTTCAGCCCCAGCAGCAGCCCCGCACGATCGA
GCGCAAGGATCTGCAGTATATAACCATCAGTAAAGAGGAGCTGTCCTCTGGTGATAGTCT
GTCTCCAGACCCCTGGAAACGAGATGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCA
CATTGTGGACATGCTAAGCAAGGAGATCCACGAGCTGCAAAACAAGGTGGACCGTACTGC
GGAGGAGAGTGACCGCCTGCGGAAGCTTATGCTGGAGTGGCAGTTCCAGAAGAGACTACA
GGAGTCCAAACAGAAGGATGAGGATGATGACGAAGAGGAAGATGATGATGTGGACACCAT
GCTGATCATGCAGCGTCTGGAGGCCGAGCGGAGAGCCAGGATGCAGGATGAGGAGCGCAG
GCGCCAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGCAGAAGATCGAGTAAGACAAGA
GGAAGATGGACGCCATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCG
ACAGGAGGAAGGGTATTACAGCCGCCTAGAAGCTGAGAGGCGCCGACAGCACGAAGAGGC
AGCACGCAGGCTGCTGGAGCCTGAAGAGCCCGGGCTGAGCCGACCTCCACTTCCACGGGA
CTATGAGCCCCCGTCCTTGTCCTCAGCACCCTGTGCCCCTCCTCCCCCACCTCAGCGAAA
CGCATCCTACCTCAAAACACAGGTCCTCTCCCCAGACTCGCTGTTCACTGCCAAGTTTGT
TGCGTATGATGAGGAGGAGGAGGACTACGGCCCAGCAGGACCAAACTCTTACTCGGGATC
TGCAGGCACAGCTGTGGGAGCCTATGATGCCCCTCGGGAAGCGAGAGAGAAACTGACTAG
GAGCCAAGACGCAGACTTACCTGGCAGTTCTGGAGCCCCTGAAAATCTGACATTTAAAGA
GCGTCAGCGCCTTTTTTCACAAGGGCAGGATGTGTCTGACAAAGTGAAAGCTTCTCGTAA
ATTAACAGAGCTCGAGAATGAACTGAACACAAAGTAGGGCCTCATGGGCCCAGCTTTCTT
GTAC
Protein sequence predicted based on nucleotide sequence
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCI RVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKD DREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQA SDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSS DGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARV MLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVE GKSLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYR LQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISET TMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDV HSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQEYRRRENRTQDATGPELI LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERT HKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLA HLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNA ALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAAD CHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIE NVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLC QRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENAELAQPLRKEPEIITVTLKKQNGMGL SIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTR TSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGS PESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGPYTSGTAAKITSV STGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQSQWPNYEEKPHVHT ESNHSSIAIQRVTRSQEELREEKVYQLERHRVEAGMDRKCDSDMWINQSSSVESSTSSQE HLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPVHYTSEFD GIPMDLPLPPPPANQAGPQSAQVAAAEWKKREEHQRWYEKEKARLEEERERKRREQERKL GQMRSQTLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERKDLQYITISK EELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKVDRTAEESDRLRKLML EWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARMQDEERRRQQQLEEMRKR EAEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPG LSRPPLPRDYEPPSLSSAPCAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDEEEEDYGP AGPNSYSGSAGTAVGAYDAPREAREKLTRSQDADLPGSSGAPENLTFKERQRLFSQGQDV SDKVKASRKLTELENELNTK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
134
End on reference sequence
5596
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
