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Mllt4 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Mllt4
Gene Name:  myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
Sequence             
Original Clone ID: FLH481792.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00297473

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 5463nts         Open reading frame : 35 to 5497

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCGGCGGGCGGCCGCGACGAAGA
GCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACCGGCTGGACCTGTTCGA
GATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTTTCAAGA
TAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGAGTCTCTAGCACAGCCACCACTCA
AGATGTGATTGAAACACTGGCAGAGAAGTTCCGCCCTGACATGCGCATGCTCTCCTCTCC
CAAGTACTCCCTCTATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAA
ACCTCTAGTTGTACAGTTGAATTGGAACAAAGATGATCGAGAGGGTCGATTCGTCCTTAA
GAACGAGAATGACGCCATTCCTGCCAAGAAGGCTCAGAGTAATGGACCTGAAAAGCAGGA
GAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTCTCAAAGAAAGAAAAGAAGGAAAA
AAAGAAGAAAGAGAAGGAGGCATTGAGACAAGCCTCTGATAAGGAAGAAAGGCCTTCTCA
AGGGGATGACAGTGAGAACTCACGACTGGCTGCTGAAGTTTACAAAGACATGCCTGAGAC
CAGCTTTACTAGGACCATTTCTAACCCTGAAGTTGTCATGAAACGGCGGCGGCAGCAAAA
ACTGGAGAAGAGAATGCAAGAGTTTCGGAGCTCAGATGGGCGGCCTGACTCAGGTGGAAC
ACTGAGGATATATGCAGATAGTTTAAAACCAAATATTCCATACAAGACAATCCTGCTGTC
TACTACCGATACCGCAGACTTTGCTGTAGCCGAATCTTTGGAGAAGTATGGTCTGGAAAA
AGAGAATCCCAAGGACTACTGTATTGCCCGGGTTATGCTTCCTCCTGGAGCCCAGCATTC
TGATGAGAGAGGTGCTAAGGAAATAATTCTTGACGATGATGAATGTCCTTTACAAATCTT
CAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGA
CTACATCCCAAAGAAAATGAAGAAACATGTTGAAGGGAAGTCGTTGAAGGGGAAAGACAG
AGCTGACGGGTCTGGCTACGGCTCCGCTCTTCCTCCCGAGAAGCTGCCCTACTTAGTAGA
GCTAAGCCCAGGGAGAAGGAATCACTTTGCCTACTACAGCTATCACACTTACGAAGATGG
CTCTGACTCCAGAGACAAGCCAAAGTTATACCGCCTTCAATTAAGTGTGACTGAAGTTGG
GACAGAGAAATTTGATGACAATTCTATCCAGCTGTTTGGCCCCGGAATTCAGCCCCATCA
CTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGATGCAGA
GACCTACGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTGCAGAGTGGCATGAGACT
GCAGTTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTC
CAAGAGATCTGTGGATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCTGT
TCAGGAGACAACCTTTGAATTGGGAGGAGATGTCCACAGTGGGACAGCATTGCCGGCAAG
CAGGAGCACTACTAGACTGGACAGTGACAGGGTGTCCTCTGCCTCTAGCACAGCTGAGCG
AGGGATGGTGAAGCCGATGATCCGACTGGACCAGGAGCAGGAATATCGACGACGAGAAAA
CAGGACTCAGGATGCTACTGGGCCTGAACTGATACTGCCTGCCAGCATTGAATTCAGGGA
AAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGTTCTACAGTTCA
TTTTAAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCA
GCACAGGCCCGACATCAGTCCTACAGAGCGTACACATAAGGCCATTGCTGTTGTCAACAA
GATGGTGAGCATGATGGAAGGGGTCATCCAGAAACAGAAGAACATTGCAGGAGCACTTGC
TTTTTGGATGGCAAATGCATCTGAGCTTCTCAACTTCATCAAGCAGGACCGAGACCTTAG
TCGGATCACGCTGGATGCCCAGGATGTCCTAGCACACTTGGTTCAGATGGCATTTAAGTA
CTTGGTTCATTGCCTTCAGTCAGAACTTAATAACTACATGCCAGCCTTTCTGGATGATCC
TGAGGAGAACAGTCTGCAAAGACCAAAAATAGATGATGTGCTGCACACACTCACAGGAGC
CATGTCCTTGCTGCGGCGCTGCAGAGTCAATGCTGCCTTGACCATCCAGCTCTTCTCTCA
GCTTTTCCACTTCATCAATATGTGGCTGTTCAACAGACTGGTGACTGACCCAGATTCAGG
CCTGTGCTCCCACTACTGGGGTGCCATTATCCGCCAGCAGCTGGGGCATATTGAAGCGTG
GGCAGAGAAGCAGGGGCTAGAGCTGGCAGCTGACTGTCACTTGAGCAGAATTGTACAGGC
TACAACATTGCTTACGATGGATAAGTATGTACCTGATGACATTCCAAATATAAACAGCAC
ATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGCCTTGTTACAAAATTACCACTGTGCACC
CGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTTGTGGCTGTTGCTGAGAACAC
AGCTGATGAGCTCGCCCGCAGTGATGGCAGAGACGTGCAGCTGGAGGAAGACCCAGACCT
ACAGCTGCCTTTTCTTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCC
AAATGGTTTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGTAGATTGGTTCC
TCACACACGTTCACCAGGTACCTGGACAATATATTTTGAAGGAGCAGATTATGAAAGTCA
CCTTATGCGTGAGAACGCAGAACTGGCCCAGCCTCTGAGGAAGGAGCCTGAGATAATCAC
TGTGACTCTGAAGAAGCAGAATGGAATGGGCCTCAGCATCGTTGCAGCCAAGGGTGCTGG
CCAGGATAAACTGGGAATCTACGTTAAGTCGGTTGTCAAAGGAGGTGCTGCAGATGTGGA
TGGACGATTAGCTGCTGGTGACCAACTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACT
TTCTCAGGAAAGGGCAGCAGAACTCATGACAAGAACCAGTTCCGTGGTTACACTGGAAGT
TGCAAAGCAAGGTGCCATCTATCACGGCCTGGCCACCCTCCTTAACCAGCCGTCCCCCAT
GATGCAAAGAATTTCAGATCGTCGTGGCTCAGGTAAACCCAGACCAAAGAGTGAAGGTTT
TGAGCTCTATAATAATTCAGCTCAAAATGGCTCCCCAGAGAGTCCTCAGATGCCTTGGAC
AGAGTACAGTGAGCCAAAGAAACTGCCTGGGGATGACAGGCTGATGAAAAACAGAGCGGA
CCACCGTTCCAGCCCCAATGTGGCAAATCAGCCTCCTAGCCCTGGAGGGAAGGGTCCATA
TACATCTGGAACAGCAGCTAAGATAACATCTGTCTCCACTGGAAACCTCTGCACTGAGGA
GCAGTCCCCTCCACCCAGACCTGAAGCCTATCCTATCCCTACTCAGACATACACCAGAGA
GTATTTTACCTTTCCAGCTTCAAAGTCGCAGGATCGGATGGCTCCTCCTCAGAGCCAGTG
GCCAAATTATGAGGAAAAACCACATGTGCACACAGAAAGTAATCATTCCAGTATTGCAAT
CCAGCGTGTTACCCGTTCCCAGGAAGAGCTTCGGGAAGAAAAGGTTTACCAACTTGAGCG
GCATCGAGTAGAGGCAGGCATGGATCGCAAGTGTGACAGTGATATGTGGATCAATCAGAG
CTCCTCAGTGGAATCCAGCACATCCAGCCAGGAACACCTGAACCATTCTTCCAAGTCAGT
TACCCCTGCTTCCACTCTGACCAAGAGTGGTCCTGGCCGTTGGAAAACACCAGCAGCGGT
GCTGCCCACCCCTGTGGCTGTCTCTCAGCCCATTCGAACAGACCTCCCTCCACCACCACC
TCCACCGCCGGTCCACTACACCAGTGAGTTTGATGGTATTCCAATGGATTTGCCCCTCCC
ACCTCCTCCTGCCAACCAGGCAGGACCCCAGTCTGCTCAGGTGGCTGCTGCAGAGTGGAA
AAAGAGAGAGGAGCACCAGAGGTGGTATGAGAAAGAGAAGGCCCGCCTGGAGGAAGAACG
AGAGAGAAAGCGCAGGGAGCAGGAGCGGAAGTTGGGGCAGATGCGCTCTCAGACCCTGAA
CCCTGCTTCCTTCTCACCTTTGGCCACACAGGCCAAGCCAGAAAAGCCTTCCACACTGCA
GAGGCCCCAAGAAACAGTCATTCGGGAGCTTCAGCCCCAGCAGCAGCCCCGCACGATCGA
GCGCAAGGATCTGCAGTATATAACCATCAGTAAAGAGGAGCTGTCCTCTGGTGATAGTCT
GTCTCCAGACCCCTGGAAACGAGATGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCA
CATTGTGGACATGCTAAGCAAGGAGATCCACGAGCTGCAAAACAAGGTGGACCGTACTGC
GGAGGAGAGTGACCGCCTGCGGAAGCTTATGCTGGAGTGGCAGTTCCAGAAGAGACTACA
GGAGTCCAAACAGAAGGATGAGGATGATGACGAAGAGGAAGATGATGATGTGGACACCAT
GCTGATCATGCAGCGTCTGGAGGCCGAGCGGAGAGCCAGGATGCAGGATGAGGAGCGCAG
GCGCCAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGCAGAAGATCGAGTAAGACAAGA
GGAAGATGGACGCCATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCG
ACAGGAGGAAGGGTATTACAGCCGCCTAGAAGCTGAGAGGCGCCGACAGCACGAAGAGGC
AGCACGCAGGCTGCTGGAGCCTGAAGAGCCCGGGCTGAGCCGACCTCCACTTCCACGGGA
CTATGAGCCCCCGTCCTTGTCCTCAGCACCCTGTGCCCCTCCTCCCCCACCTCAGCGAAA
CGCATCCTACCTCAAAACACAGGTCCTCTCCCCAGACTCGCTGTTCACTGCCAAGTTTGT
TGCGTATGATGAGGAGGAGGAGGACTACGGCCCAGCAGGACCAAACTCTTACTCGGGATC
TGCAGGCACAGCTGTGGGAGCCTATGATGCCCCTCGGGAAGCGAGAGAGAAACTGACTAG
GAGCCAAGACGCAGACTTACCTGGCAGTTCTGGAGCCCCTGAAAATCTGACATTTAAAGA
GCGTCAGCGCCTTTTTTCACAAGGGCAGGATGTGTCTGACAAAGTGAAAGCTTCTCGTAA
ATTAACAGAGCTCGAGAATGAACTGAACACAAAGTAGGGCCTCATGGGCCCAGCTTTCTT
GTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 5596
Start on reference sequence 134
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Mllt4
Symbol Nomenclature : Mllt4
SYNONYM : 5033403D15Rik; AF6; Af-6; Afadin; Gm314; I-afadin; S-afadin
Designation : afadin|myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog|protein Af-6
Full Nomenclature : myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
GENEID : 17356
GI : 182888520
GenBank Accession : BC160377
Mouse Genome Informatics : 1314653
Vega : OTTMUSG00000029503
Target GenBank: NM_010806.1

 

Clone

Cloning Information : 481792
HIP Master Clone ID : 399520
IMAGE ID : 100064121
Original Clone ID : FLH481792.01X

Organism


TAX_ID : 10090
Species Specific ID: 17356

Genome

Chromosome : 17
Map Location : 17 A2|17
Ensembl : ENSMUSG00000068036

Reagents

Labome : Mllt4-antibody