PTPRN2 (H. sapiens )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PTPRN2
Gene Name:  protein tyrosine phosphatase, receptor type, N polypeptide 2
Original Clone ID:
8621a8BCt8p1
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HsCD00297758
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 930nts
Open reading frame: 48 to 977

TCTGAATATTTTGTTTAACTTTAGAAGGAGATATACATATGTCCCTTTCCGAGGAGCCTG
TGCAGTCCAACATGGACATCTCCACCGGCCACATGATCCTGTCCTACATGGAGGACCACC
TGAAGAACAAGAACCGGCTGGAGAAGGAGTGGGAAGCGCTGTGCGCCTACCAGGCGGAGC
CCAACAGCTCGTTCGTGGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCCCTGGCTG
TGCTGACCTATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCAG
ACTACATCAACGCTAGCCCTATCATGGATCACGACCCGAGGAACCCGGCGTACATCGCCA
CCCAGGGACCGCTGCCCGCCACCGTGGCTGACTTTTGGCAGATGGTGTGGGAGAGCGGCT
GCGTGGTGATCGTCATGCTGACACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTACCACT
ACTGGCCGGATGAAGGCTCCAATCTCTACCACATCTATGAGGTGAACCTGGTCTCCGAGC
ACATCTGGTGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGAAGAACCTGCAGACCAACG
AGACGCGCACCGTGACGCAGTTCCACTTCCTGAGTTGGTATGACCGAGGAGTCCCTTCCT
CCTCAAGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACAAGTGCTACAGGGGCCGTTCTT
GTCCAATAATTGTTCATTGCAGTGACGGTGCAGGCCGGAGCGGCACCTACGTCCTGATCG
ACATGGTTCTCAACAAGATGGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGACCCTGG
AGCACTTGAGGGACCAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCGC
TGACAGCCGTGGCTGAGGAGGTGAACGCCATCCTCAAGGCCCTTCCCCAGGAAGGGCATC
ATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG
CACCAC

Protein sequence predicted based on nucleotide sequence

SEEPVQSNMDISTGHMILSYMEDHLKNKNRLEKEWEALCAYQAEPNSSFVAQREENVPKN
RSLAVLTYDHSRVLLKAENSHSHSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMV
WESGCVVIVMLTPLAENGVRQCYHYWPDEGSNLYHIYEVNLVSEHIWCEDFLVRSFYLKN
LQTNETRTVTQFHFLSWYDRGVPSSSRSLLDFRRKVNKCYRGRSCPIIVHCSDGAGRSGT
YVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAILKALP
QEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 930
Mutations:
None
Discrepancy:
"c273t,P91P;c300g,P100P;"
                                                    

5' Linker Sequence:
ATGTCCCTT
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-8621a
Recommended expression in:
Not Applicable