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Detailed Plasmid Information

TOP

Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 9095b1BCt3p1
Keyword: None
Species: Trypanosoma brucei
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: C-terminal 6-His, TOPO
Mutation/
Discrepancy :
No / No
Publications: PMID: 18058037
Title: Structural genomics of protein phosphatases
Authors: PSI
New York SGX Research Center for Structural Genomics
TbCD00297943

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 780nts         Open reading frame : 50 to 829

TCTAGAAATATTTTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAAAGTGCAGGG
CTATGCGAATGTGGTGACCCTGCCGAACGTAACGGGTCGTGTGATTATCGTTGGGGATAT
TCATGGATGCCGCGCCCAACTGGAGGACCTGCTGCGTGCAGTCAGCTTTAAACAGGGCTC
TGATACTCTGGTAGCTGTGGGTGACCTGGTTAATAAAGGGCCAGATAGTTTCGGAGTCGT
GCGCCTGCTGAAACGTCTGGGCGCGTACTCAGTACTGGGTAACCACGACGCCAAACTGCT
GAAACTGGTTAAAAAACTGGGCAAAAAAGAGTGTCTGAAAGGTCGCGATGCAAAATCCTC
GCTGGCTCCTCTGGCGCAAAGCATCCCGACAGATGTGGAAACCTATCTGTCTCAGCTGCC
ACATATTATCCGTATTCCTGCCCACAATGTCATGGTTGCACATGCTGGGCTGCACCCGCA
GCGCCCGGTAGACCGTCAATACGAGGATGAAGTGACGACTATGCGCAACCTGATCGAAAA
AGAGCAGGAAGCGACTGGAGGCGTCACCCTGACGGCCACCGAGGAAACTAATGACGGTGG
CAAACCATGGGCAAGTATGTGGCGTGGTCCTGAAACAGTTGTGTTTGGGCATGATGCTCG
CCGTGGACTGCAAGAGCAGTATAAACCGCTGGCGATTGGCCTGGACTCACGCTGCGTATA
CGGTGGGCGTCTGTCGGCTGCGGTATTCCCGGGCGGTTGTATCATTTCGGTTCCAGGATG
GAATGGCGCGTCTGCCGCTGCAGAAGGGCATCATCACCATCACCACTGATCCGAATTCGA
GCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 780
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: SolubleProtein: Protein_Soluble
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in NYSGXRC-9095b
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental

Gene

Target GenBank: XP_847629

 

Protein

PDB - Structural Annotation : 2QJC
Structural Annotation Wiki : TOPSAN
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 495982
HIP Master Clone ID : 409662
Original Clone ID : 9095b1BCt3p1

Experimental

Structural Biology Knowledgebase : SBKB (2QJC)
TargetTrack - Experimental data : NYSGXRC-9095b