Original Clone ID:
FLH230776.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
FUSION
Source:
HIP
Description:
None
Comments:
This clone is identical to HsCD00618033
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00298641
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT
AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA
GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG
GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT
GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT
CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG
TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT
ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA
ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG
AGGGAGAAGCACAAGATCATGCACAGAGCATACGGAATGGACAGCCGACCTCCCATGGCA
ATTTTTGAGTTGTTGGATTACATAGTCAACGAGCCTCCTCCAAAACTGCCCAGTGGAGTG
TTCAGTCTGGAATTTCAAGATTTTGTGAATAAATGCTTAATAAAAAACCCCGCAGAGAGA
GCAGATTTGAAGCAACTCATGGTTCATGCTTTTATCAAGAGATCTGATGCTGAGGAAGTG
GATTTTGCAGGTTGGCTCTGCTCCACCATCGGCCTTAACCAGCCCAGCACACCAACCCAT
GCTGCTGGCGTCTTG
Protein sequence predicted based on nucleotide sequence
MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKV GELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHE CNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYL REKHKIMHRAYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAER ADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
1254
Start on reference sequence
73
Mutations:
None
Discrepancy:
del@569,299;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
Gene
Gene Symbol :
MAP2K1
Symbol Nomenclature :
MAP2K1
SYNONYM :
CFC3; MAPKK1; MEK1; MKK1; PRKMK1
Designation :
ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|dual specificity mitogen-activated protein kinase kinase 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Full Nomenclature :
mitogen-activated protein kinase kinase 1
GENEID :
SYNONYM :
HGNC :
MIM :
Target GenBank:
Reference Sequence Alignment
HsCD00298641 ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT NM_002755.3 ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT XM_005254541.1 ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT ************************************************************ HsCD00298641 AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA NM_002755.3 AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA XM_005254541.1 AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA ************************************************************ HsCD00298641 GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG NM_002755.3 GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG XM_005254541.1 GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG ************************************************************ HsCD00298641 GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT NM_002755.3 GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT XM_005254541.1 GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT ************************************************************ HsCD00298641 GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT NM_002755.3 GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT XM_005254541.1 GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT ************************************************************ HsCD00298641 CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG NM_002755.3 CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG XM_005254541.1 CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG ************************************************************ HsCD00298641 TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT NM_002755.3 TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT XM_005254541.1 TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT ************************************************************ HsCD00298641 ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA NM_002755.3 ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA XM_005254541.1 ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA ************************************************************ HsCD00298641 ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG NM_002755.3 ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG XM_005254541.1 ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG ************************************************************ HsCD00298641 AGGGAGAAGCACAAGATCATGCACAGAGCATACGG------------------------- NM_002755.3 AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC XM_005254541.1 AGGGAGAAGCACAAGATCATGCACAGAGCATACGG------------------------- ****************************.: :*.. HsCD00298641 ------------------------------------------------------------ NM_002755.3 CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC XM_005254541.1 ------------------------------------------------------------ HsCD00298641 ------------------------------------------------------------ NM_002755.3 AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC XM_005254541.1 ------------------------------------------------------------ HsCD00298641 ------------------------------------------------------------ NM_002755.3 TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG XM_005254541.1 ------------------------------------------------------------ HsCD00298641 ------------------------------------------------------------ NM_002755.3 TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA XM_005254541.1 ------------------------------------------------------------ HsCD00298641 ------------------------------------------------------------ NM_002755.3 GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC XM_005254541.1 ------------------------------------------------------------ HsCD00298641 --AATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG NM_002755.3 GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG XM_005254541.1 --AATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG ********************************************************** HsCD00298641 CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA NM_002755.3 CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA XM_005254541.1 CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA ************************************************************ HsCD00298641 TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT NM_002755.3 TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT XM_005254541.1 TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT ************************************************************ HsCD00298641 ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC NM_002755.3 ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC XM_005254541.1 ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC ************************************************************ HsCD00298641 CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTTG NM_002755.3 CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAA XM_005254541.1 CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAA ****************************************:.
Pathways
NCI :
BCR signaling pathway
NCI :
Cellular roles of Anthrax toxin
NCI :
Ceramide signaling pathway
NCI :
Class IB PI3K non-lipid kinase events
NCI :
CXCR3-mediated signaling events
NCI :
Downstream signaling in naïve CD8+ T cells
NCI :
Endothelins
NCI :
EPHB forward signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
FOXM1 transcription factor network
NCI :
GMCSF-mediated signaling events
NCI :
Hedgehog signaling events mediated by Gli proteins
NCI :
IFN-gamma pathway
NCI :
IL2-mediated signaling events
NCI :
mTOR signaling pathway
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Nongenotropic Androgen signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Ras signaling in the CD4+ TCR pathway
NCI :
SHP2 signaling
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Trk receptor signaling mediated by the MAPK pathway
Panther :
Angiogenesis
Panther :
Angiotensin II-stimulated signaling through G proteins and beta-arrestin
Panther :
B cell activation
Panther :
EGF receptor signaling pathway
Panther :
Endothelin signaling pathway
Panther :
FGF signaling pathway
Panther :
Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade
Panther :
Integrin signalling pathway
Panther :
PDGF signaling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
Toll receptor signaling pathway
Panther :
VEGF signaling pathway
Reactome :
Activated TLR4 signalling
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling of activated FGFR
Reactome :
ERK activation
Reactome :
ERK1 activation
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin-1 signaling
Reactome :
Interleukin-2 signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
L1CAM interactions
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MEK activation
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
NCAM signaling for neurite out-growth
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Prolonged ERK activation events
Reactome :
RAF phosphorylates MEK
Reactome :
RAF/MAP kinase cascade
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
EPO Receptor Signaling
WikiPathway :
ErbB signaling pathway
WikiPathway :
Estrogen signaling pathway
WikiPathway :
Focal Adhesion
WikiPathway :
Hypothetical Network for Drug Addiction
WikiPathway :
IL-1 signaling pathway
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
IL-5 signaling pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
IL-7 signaling pathway
WikiPathway :
IL-9 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK Cascade
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
Nifedipine Activity
WikiPathway :
Osteopontin Signaling
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
Senescence and Autophagy
WikiPathway :
Serotonin HTR1 Group and FOS Pathway
WikiPathway :
Serotonin Receptor 2 and ELK-SRF/GATA4 signaling
WikiPathway :
Serotonin Receptor 4/6/7 and NR3C Signaling
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TSH signaling pathway
Protein
SMART domain :
SMART domain :
UniProt :
UniProt :
HPRD :
01469
Clone
Organism
Species Specific ID:
Genome
Chromosome :
15
Map Location :
15q22.1-q22.33
Reagents
Labome :