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GTF2F1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2F1
Gene Name:  general transcription factor IIF, polypeptide 1, 74kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH014780.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002990

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1554nts         Open reading frame : 1 to 1554
Reference Sequence Alignment

ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1701
Start on reference sequence 148
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2F1
Symbol Nomenclature : GTF2F1
SYNONYM : BTF4; RAP74; TF2F1; TFIIF
Designation : TFIIF-alpha|general transcription factor IIF 74 kDa subunit|general transcription factor IIF subunit 1|transcription initiation factor IIF subunit alpha|transcription initiation factor RAP74
Full Nomenclature : general transcription factor IIF, polypeptide 1, 74kDa
GENEID : 2962
GI : 60655298
GenBank Accession : AY889422
HGNC : 4652
MIM : 189968
Vega : OTTHUMG00000168087
Target GenBank: BC000120

 

Reference Sequence Alignment

  

HsCD00002990      ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
NM_002096.2       ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
                  ************************************************************

HsCD00002990      AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
NM_002096.2       AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
                  ************************************************************

HsCD00002990      ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
NM_002096.2       ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
                  ************************************************************

HsCD00002990      GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
NM_002096.2       GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
                  ************************************************************

HsCD00002990      AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
NM_002096.2       AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
                  ************************************************************

HsCD00002990      CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
NM_002096.2       CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
                  ************************************************************

HsCD00002990      AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
NM_002096.2       AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
                  ************************************************************

HsCD00002990      CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
NM_002096.2       CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
                  ************************************************************

HsCD00002990      GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
NM_002096.2       GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
                  ************************************************************

HsCD00002990      CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
NM_002096.2       CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
                  ************************************************************

HsCD00002990      AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
NM_002096.2       AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
                  ************************************************************

HsCD00002990      AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
NM_002096.2       AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
                  ************************************************************

HsCD00002990      GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
NM_002096.2       GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
                  ************************************************************

HsCD00002990      AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
NM_002096.2       AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
                  ************************************************************

HsCD00002990      TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
NM_002096.2       TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
                  ************************************************************

HsCD00002990      GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
NM_002096.2       GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
                  ************************************************************

HsCD00002990      AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
NM_002096.2       AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
                  ************************************************************

HsCD00002990      AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
NM_002096.2       AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
                  ************************************************************

HsCD00002990      CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
NM_002096.2       CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
                  ************************************************************

HsCD00002990      TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
NM_002096.2       TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
                  ************************************************************

HsCD00002990      CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
NM_002096.2       CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
                  ************************************************************

HsCD00002990      AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
NM_002096.2       AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
                  ************************************************************

HsCD00002990      CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
NM_002096.2       CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
                  ************************************************************

HsCD00002990      CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
NM_002096.2       CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
                  ************************************************************

HsCD00002990      AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
NM_002096.2       AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
                  ************************************************************

HsCD00002990      AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAGTAG
NM_002096.2       AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAGTGA
                  ****************************************************..

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : Abortive elongation of HIV-1 transcript in the absence of Tat
Reactome : Disease
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : HIV elongation arrest and recovery
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Influenza Viral RNA Transcription and Replication
Reactome : Late Phase of HIV Life Cycle
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Viral Messenger RNA Synthesis
Reactome : mRNA Capping
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
Reactome : mRNA Splicing - Minor Pathway

Protein

UniProt : P35269
HPRD : 01803

Clone

Cloning Information : 14780
HIP Master Clone ID : 28979
Original Clone ID : FLH014780.01X

Organism


TAX_ID : 9606
Species Specific ID: 2962

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000125651

Reagents

Labome : GTF2F1-antibody