Gene:
Gene Symbol:
TAX1BP1
Gene Name:
Tax1 (human T-cell leukemia virus type I) binding protein 1
Original Clone ID:
FLH271353.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
This clone is identical to HsCD00617891
Mutation / Discrepancy:
No / No
Publications:
PMID: 24817723
Title: Expression, purification and crystallization of the SKICH domain of human TAX1BP1
Title: Expression, purification and crystallization of the SKICH domain of human TAX1BP1
Authors:
HsCD00300502
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACATCCTTTCAAGAAGTCCCATTGCAGACTTCCAACTTTGCCCATGTCATCTTTCAA
AATGTGGCCAAGAGTTACCTTCCTAATGCACACCTGGAATGTCATTACACCTTAACTCCA
TATATTCATCCACATCCAAAAGATTGGGTTGGTATATTCAAGGTTGGATGGAGTACTGCT
CGTGATTATTACACGTTTTTATGGTCCCCTATGCCTGAACATTATGTGGAAGGATCAACA
GTCAATTGTGTACTAGCATTCCAAGGATATTACCTTCCAAATGATGATGGAGAATTTTAT
CAGTTCTGTTACGTTACCCATAAGGGTGAAATTCGTGGAGCAAGTACACCTTTCCAGTTT
CGAGCTTCTTCTCCAGTTGAAGAGCTGCTTACTATGGAAGATGAAGGAAATTCTGACATG
TTAGTGGTGACCACAAAAGCAGGCCTTCTTGAGTTGAAAATTGAGAAAACCATGAAAGAA
AAAGAAGAACTGTTAAAGTTAATTGCCGTTCTGGAAAAAGAAACAGCACAACTTCGAGAA
CAAGTTGGGAGAATGGAAAGAGAACTTAACCATGAGAAAGAAAGATGTGACCAACTGCAA
GCAGAACAAAAGGGTCTTACTGAAGTAACACAAAGCTTAAAAATGGAAAATGAAGAGTTT
AAGAAGAGGTTCAGTGATGCTACATCCAAAGCCCATCAGCTTGAGGAAGATATTGTGTCA
GTAACACATAAAGCAATTGAAAAAGAAACCGAATTAGACAGTTTAAAGGACAAACTCAAG
AAGGCACAACATGAAAGAGAACAACTTGAATGTCAGTTGAAGACAGAGAAGGATGAAAAG
GAACTTTATAAGGTACATTTGAAGAATACAGAAATAGAAAATACCAAGCTTATGTCAGAG
GTCCAGACTTTAAAAAATATAGATGGGAACAAAGAAAGCGTGATTACTCATTTCAAAGAA
GAGATTGGCAGGCTGCAGTTATGTTTGGCTGAAAAGGAAAATCTGCAAAGAACTTTCCTG
CTTACAACCTCAAGTAAAGAAGATACTTGTTTTTTAAAGGAGCAACTTCGTAAAGCAGAG
GAACAGGTTCAGGCAACTCGGCAAGAAGTTGTCTTTCTGGCTAAAGAACTCAGTGATGCT
GTCAACGTACGAGACAGAACGATGGCAGACCTGCATACTGCACGCTTGGAAAACGAGAAA
GTGAAAAAGCAGTTAGCTGATGCAGTGGCAGAACTTAAACTAAATGCTATGAAAAAAGAT
CAGGACAAGACTGATACACTGGAACACGAACTAAGAAGAGAAGTTGAAGATCTGAAACTC
CGTCTTCAGATGGCTGCAGACCATTATAAAGAAAAATTTAAGGAATGCCAAAGGCTCCAA
AAACAAATAAACAAACTTTCAGATCAATCAGCTAATAATAATAATGTCTTCACAAAGAAA
ACGGGGAATCAGCAGAAAGTGAATGATGCTTCAGTAAACACAGACCCAGCCACTTCTGCC
TCTACTGTAGATGTAAAGCCATCACCTTCTGCAGCAGAGGCAGATTTTGACATAGTAACA
AAGGGGCAAGTCTGTGAAATGACCAAAGAAATTGCTGACAAAACAGAAAAGTATAATAAA
TGTAAACAACTCTTGCAGGATGAGAAAGCAAAATGCAATAAATATGCTGATGAACTTGCA
AAAATGGAGCTGAAATGGAAAGAACAAGTGAAAATTGCTGAAAATGTAAAACTTGAACTA
GCTGAAGTACAGGACAATTATAAAGAACTTAAAAGGAGTCTAGAAAATCCAGCAGAAAGG
AAAATGGAAGGTCAGAATTCCCAGAGTCCTCAATGTTTCAAAACATGCTCAGAGCAAAAT
GGTTATGTTCTCACATTGTCAAATGCACAACCAGTTCTGCAATATGGTAATCCTTATGCA
TCTCAGGAAACAAGAGATGGAGCAGATGGTGCTTTTTACCCAGATGAAATACAAAGGCCA
CCTGTCAGAGTCCCCTCTTGGGGACTGGAAGACAATGTTGTCTGCAGCCAGCCTGCTCGA
AACTTTAGTCGGCCTGATGGCTTAGAGGACTCTGAGGATAGCAAAGAAGATGAGAATGTG
CCTACTGCTCCTGATCCTCCAAGTCAACATTTACGTGGGCATGGGACAGGCTTTTGCTTT
GATTCCAGCTTTGATGTTCACAAGAAGTGTCCCCTCTGTGAGTTAATGTTTCCTCCTAAC
TATGATCAGAGCAAATTTGAAGAACATGTTGAAAGTCACTGGAAGGTGTGCCCGATGTGC
AGCGAGCAGTTCCCTCCTGACTATGACCAGCAGGTGTTTGAAAGGCATGTGCAGACCCAT
TTTGATCAGAATGTTCTAAATTTTGACTAG
Protein sequence predicted based on nucleotide sequence
MTSFQEVPLQTSNFAHVIFQNVAKSYLPNAHLECHYTLTPYIHPHPKDWVGIFKVGWSTA RDYYTFLWSPMPEHYVEGSTVNCVLAFQGYYLPNDDGEFYQFCYVTHKGEIRGASTPFQF RASSPVEELLTMEDEGNSDMLVVTTKAGLLELKIEKTMKEKEELLKLIAVLEKETAQLRE QVGRMERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKKRFSDATSKAHQLEEDIVS VTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKELYKVHLKNTEIENTKLMSE VQTLKNIDGNKESVITHFKEEIGRLQLCLAEKENLQRTFLLTTSSKEDTCFLKEQLRKAE EQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENEKVKKQLADAVAELKLNAMKKD QDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQRLQKQINKLSDQSANNNNVFTKK TGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFDIVTKGQVCEMTKEIADKTEKYNK CKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENVKLELAEVQDNYKELKRSLENPAER KMEGQNSQSPQCFKTCSEQNGYVLTLSNAQPVLQYGNPYASQETRDGADGAFYPDEIQRP PVRVPSWGLEDNVVCSQPARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCF DSSFDVHKKCPLCELMFPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTH FDQNVLNFD*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
2437
Start on reference sequence
68
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
