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ATP5J (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ATP5J
Gene Name:  ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH014435.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003024

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 327nts         Open reading frame : 1 to 327
Reference Sequence Alignment

ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTCATTCGGTCAGCCGTCTCAGTCCAT
TTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTTAATAAGGAACTTGATCCTATACAG
AAACTCTTTGTGGACAAGATTAGAGAATACAAATCTAAGCGACAGACATCTGGAGGACCT
GTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAGAGGGAGCTTTTTAAGCTCAAGCAA
ATGTTTGGTAATGCAGACATGAATACATTTCCCACCTTCAAATTTGAAGATCCCAAATTT
GAAGTCATCGAAAAACCCCAGGCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1019
Start on reference sequence 693
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ATP5J
Symbol Nomenclature : ATP5J
SYNONYM : ATP5; ATP5A; ATPM; CF6; F6
Designation : ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6|ATP synthase-coupling factor 6, mitochondrial|ATPase subunit F6|coupling factor 6|mitochondrial ATP synthase, coupling factor 6|mitochondrial ATP synthase, subunit F6|mitochondrial ATPase coupling factor 6|proliferation-inducing protein 36
Full Nomenclature : ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
GENEID : 522
GI : 61372422
GenBank Accession : AY891914
HGNC : 847
MIM : 603152
Vega : OTTHUMG00000078442
Target GenBank: BC001178

 

Reference Sequence Alignment

  

HsCD00003024        ------------------------ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTC
NM_001003696.1      ------------------------ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTC
NM_001003697.1      ------------------------ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTC
NM_001003701.1      ATGCATTGCGATGGCGGAATCAGCATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTC
NM_001003703.1      ------------------------ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTC
NM_001685.4         ------------------------ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTC
                                            ************************************

HsCD00003024        ATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTT
NM_001003696.1      ATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTT
NM_001003697.1      ATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTT
NM_001003701.1      ATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTT
NM_001003703.1      ATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTT
NM_001685.4         ATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTT
                    ************************************************************

HsCD00003024        AATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCT
NM_001003696.1      AATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCT
NM_001003697.1      AATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCT
NM_001003701.1      AATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCT
NM_001003703.1      AATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCT
NM_001685.4         AATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCT
                    ************************************************************

HsCD00003024        AAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAG
NM_001003696.1      AAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAG
NM_001003697.1      AAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAG
NM_001003701.1      AAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAG
NM_001003703.1      AAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAG
NM_001685.4         AAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAG
                    ************************************************************

HsCD00003024        AGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACC
NM_001003696.1      AGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACC
NM_001003697.1      AGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACC
NM_001003701.1      AGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACC
NM_001003703.1      AGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACC
NM_001685.4         AGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACC
                    ************************************************************

HsCD00003024        TTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACCCCAGGCCTTG
NM_001003696.1      TTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACCCCAGGCCTGA
NM_001003697.1      TTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACCCCAGGCCTGA
NM_001003701.1      TTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACCCCAGGCCTGA
NM_001003703.1      TTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACCCCAGGCCTGA
NM_001685.4         TTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACCCCAGGCCTGA
                    ************************************************* .

Pathways

NCI : FOXA1 transcription factor network
NCI : Validated nuclear estrogen receptor alpha network
Reactome : Formation of ATP by chemiosmotic coupling
Reactome : Metabolism
Reactome : Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
Reactome : The citric acid (TCA) cycle and respiratory electron transport
WikiPathway : Electron Transport Chain
WikiPathway : Oxidative phosphorylation

Protein

UniProt : Q6IB54
UniProt : Q6NZ59
UniProt : P18859
HPRD : 04399

Clone

Cloning Information : 14435
HIP Master Clone ID : 28634
Original Clone ID : FLH014435.01L

Organism


TAX_ID : 9606
Species Specific ID: 522

Genome

Chromosome : 21
Map Location : 21q21.1
Ensembl : ENSG00000154723

Reagents

Labome : ATP5J-antibody