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C20orf186 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB4
Gene Name:  BPI fold containing family B, member 4
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH469890.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00303156

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1725nts         Open reading frame : 1 to 1725
Reference Sequence Alignment

ATGCTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGT
GATATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAA
AAACTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTG
GGGGGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGA
AACAGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATAC
AGGGCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCT
GGAGTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCA
GCTGATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGAT
GGAGGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGC
ACTGTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTG
CGGCTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGG
AAGAGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGG
CTGACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTA
GGGGGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTG
AACCGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTG
GGTCTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGA
AGTGTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGAC
CTCAACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCA
GCTGTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAG
CTGGCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCT
CTAGACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACA
CTGGGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATC
AGGATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAG
GTGTTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGC
CTCATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATG
ATTGATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTT
GATATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCA
ATGAACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGC
AATGCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1728
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB4
Symbol Nomenclature : BPIFB4
SYNONYM : C20orf186; LPLUNC4; RY2G5; dJ726C3.5
Designation : BPI fold-containing family B member 4|ligand-binding protein RY2G5|likely ortholog of rat probable ligand-binding protein RY2G5|long palate, lung and nasal epithelium carcinoma associated 4|long palate, lung and nasal epithelium carcinoma-associated protein 4
Full Nomenclature : BPI fold containing family B, member 4
GENEID : 149954
HGNC : 16179
Vega : OTTHUMG00000032235
Target GenBank: NM_182519

 

Reference Sequence Alignment

  

HsCD00303156      ------------------------------------------------------------
NM_182519.2       ATGTGGATGGCCTGGTGTGTGGCTGCGCTGTCTGTGGTGGCTGTGTGTGGCACCAGCCAC
                                                                              

HsCD00303156      ---------------------------------------------------------ATG
NM_182519.2       GAGACAAACACGGTCCTCAGGGTGACGAAAGATGTGTTGAGCAATGCCATTTCAGGCATG
                                                                           ***

HsCD00303156      CTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGTGAT
NM_182519.2       CTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGTGAT
                  ************************************************************

HsCD00303156      ATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAAAAA
NM_182519.2       ATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAAAAA
                  ************************************************************

HsCD00303156      CTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTGGGG
NM_182519.2       CTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTGGGG
                  ************************************************************

HsCD00303156      GGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGAAAC
NM_182519.2       GGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGAAAC
                  ************************************************************

HsCD00303156      AGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATACAGG
NM_182519.2       AGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATACAGG
                  ************************************************************

HsCD00303156      GCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCTGGA
NM_182519.2       GCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCTGGA
                  ************************************************************

HsCD00303156      GTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCAGCT
NM_182519.2       GTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCAGCT
                  ************************************************************

HsCD00303156      GATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGATGGA
NM_182519.2       GATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGATGGA
                  ************************************************************

HsCD00303156      GGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGCACT
NM_182519.2       GGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGCACT
                  ************************************************************

HsCD00303156      GTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTGCGG
NM_182519.2       GTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTGCGG
                  ************************************************************

HsCD00303156      CTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGGAAG
NM_182519.2       CTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGGAAG
                  ************************************************************

HsCD00303156      AGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGGCTG
NM_182519.2       AGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGGCTG
                  ************************************************************

HsCD00303156      ACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTAGGG
NM_182519.2       ACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTAGGG
                  ************************************************************

HsCD00303156      GGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTGAAC
NM_182519.2       GGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTGAAC
                  ************************************************************

HsCD00303156      CGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTGGGT
NM_182519.2       CGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTGGGT
                  ************************************************************

HsCD00303156      CTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGAAGT
NM_182519.2       CTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGAAGT
                  ************************************************************

HsCD00303156      GTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGACCTC
NM_182519.2       GTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGACCTC
                  ************************************************************

HsCD00303156      AACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCAGCT
NM_182519.2       AACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCAGCT
                  ************************************************************

HsCD00303156      GTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAGCTG
NM_182519.2       GTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAGCTG
                  ************************************************************

HsCD00303156      GCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCTCTA
NM_182519.2       GCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCTCTA
                  ************************************************************

HsCD00303156      GACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACACTG
NM_182519.2       GACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACACTG
                  ************************************************************

HsCD00303156      GGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATCAGG
NM_182519.2       GGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATCAGG
                  ************************************************************

HsCD00303156      ATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAGGTG
NM_182519.2       ATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAGGTG
                  ************************************************************

HsCD00303156      TTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGCCTC
NM_182519.2       TTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGCCTC
                  ************************************************************

HsCD00303156      ATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATGATT
NM_182519.2       ATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATGATT
                  ************************************************************

HsCD00303156      GATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTTGAT
NM_182519.2       GATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTTGAT
                  ************************************************************

HsCD00303156      ATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCAATG
NM_182519.2       ATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCAATG
                  ************************************************************

HsCD00303156      AACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGCAAT
NM_182519.2       AACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGCAAT
                  ************************************************************

HsCD00303156      GCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCA---
NM_182519.2       GCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCATGA
                  ******************************************   

Protein

SMART domain : BPI1 : BPI/LBP/CETP N-terminal domain
SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
UniProt : P59827
HPRD : 16645

Clone

Cloning Information : 469890
HIP Master Clone ID : 197943
Original Clone ID : FLH469890.01L

Organism


TAX_ID : 9606
Species Specific ID: 149954

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000186191

Reagents

Labome : BPIFB4-antibody