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ASB17 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ASB17
Gene Name:  ankyrin repeat and SOCS box containing 17
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH473124.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00303228

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 888nts         Open reading frame : 1 to 888
Reference Sequence Alignment

ATGAGTAAATCTACTAAATTATGTGGTAAGACTTCTTGTCCAAGAAGCAATATATTCTGC
AATCTCCTTGACAAAATTGTTAAAAGACCCTCCCTACAGTTTTTGGGTCAGTGGGGATAT
CACTGTTACGAACCAAGGATTTACAGATCACTGGCAAAAATTCTGAGGTATGTGGACTTG
GATGGTTTTGACGCACTACTCACAGATTACATTGCATTTGTGGAAAAATCAGGATACCGT
TTTGAAGTAAGTTTTAACCTCGACTTCACTGAAATATGTGTGAATACAATTCTGTACTGG
GTTTTTGCCAGAAAAGGTAATCCTGACTTTGTGGAATTGCTTCTCAAGAAGACAAAAGAC
TATGTTCAAGACAGAAGTTGTAACCTGGCACTGATATGGAGAACTTTCACACCAGTATAC
TGTCCAAGCCCATTAAGTGGCATCACACCTCTCTTTTATGTAGCTCAGACAAGACAGTCT
AATATCTTCAAAATACTACTGCAATATGGAATCTTAGAAAGAGAAAAAAACCCTATCAAC
ATTGTCTTAACAATAGTACTCTACCCTTCGAGAGTAAGAGTAATGGTTGATCGTGAATTG
GCTGACATCCATGAAGATGCCAAAACATGTTTGGTACTATGTTCCAGAGTGCTTTCTGTC
ATTTCAGTCAAGGAAATAAAGACACAGCTGAGTTTAGGAAGACATCCAATTATTTCAAAT
TGGTTTGATTACATTCCTTCAACAAGATACAAAGATCCATGTGAACTATTACATCTTTGC
AGACTAACCATCAGGAATCAACTATTAACCAACAATATGCTCCCAGATGGAATATTTTCA
CTTCTAATTCCTGCTCGTCTACAAAACTATCTGAATTTAGAAATCTAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1006
Start on reference sequence 119
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ASB17
Symbol Nomenclature : ASB17
SYNONYM : Asb-17
Designation : ankyrin repeat and SOCS box protein 17|ankyrin repeat and SOCS box-containing 17
Full Nomenclature : ankyrin repeat and SOCS box containing 17
GENEID : 127247
HGNC : 19769
Vega : OTTHUMG00000009787
Target GenBank: BC036573.2

 

Reference Sequence Alignment

  

HsCD00303228      ATGAGTAAATCTACTAAATTATGTGGTAAGACTTCTTGTCCAAGAAGCAATATATTCTGC
NM_080868.2       ATGAGTAAATCTACTAAATTATGTGGTAAGACTTCTTGTCCAAGAAGCAATATATTCTGC
                  ************************************************************

HsCD00303228      AATCTCCTTGACAAAATTGTTAAAAGACCCTCCCTACAGTTTTTGGGTCAGTGGGGATAT
NM_080868.2       AATCTCCTTGACAAAATTGTTAAAAGACCCTCCCTACAGTTTTTGGGTCAGTGGGGATAT
                  ************************************************************

HsCD00303228      CACTGTTACGAACCAAGGATTTACAGATCACTGGCAAAAATTCTGAGGTATGTGGACTTG
NM_080868.2       CACTGTTACGAACCAAGGATTTACAGATCACTGGCAAAAATTCTGAGGTATGTGGACTTG
                  ************************************************************

HsCD00303228      GATGGTTTTGACGCACTACTCACAGATTACATTGCATTTGTGGAAAAATCAGGATACCGT
NM_080868.2       GATGGTTTTGACGCACTACTCACAGATTACATTGCATTTGTGGAAAAATCAGGATACCGT
                  ************************************************************

HsCD00303228      TTTGAAGTAAGTTTTAACCTCGACTTCACTGAAATATGTGTGAATACAATTCTGTACTGG
NM_080868.2       TTTGAAGTAAGTTTTAACCTCGACTTCACTGAAATATGTGTGAATACAATTCTGTACTGG
                  ************************************************************

HsCD00303228      GTTTTTGCCAGAAAAGGTAATCCTGACTTTGTGGAATTGCTTCTCAAGAAGACAAAAGAC
NM_080868.2       GTTTTTGCCAGAAAAGGTAATCCTGACTTTGTGGAATTGCTTCTCAAGAAGACAAAAGAC
                  ************************************************************

HsCD00303228      TATGTTCAAGACAGAAGTTGTAACCTGGCACTGATATGGAGAACTTTCACACCAGTATAC
NM_080868.2       TATGTTCAAGACAGAAGTTGTAACCTGGCACTGATATGGAGAACTTTCACACCAGTATAC
                  ************************************************************

HsCD00303228      TGTCCAAGCCCATTAAGTGGCATCACACCTCTCTTTTATGTAGCTCAGACAAGACAGTCT
NM_080868.2       TGTCCAAGCCCATTAAGTGGCATCACACCTCTCTTTTATGTAGCTCAGACAAGACAGTCT
                  ************************************************************

HsCD00303228      AATATCTTCAAAATACTACTGCAATATGGAATCTTAGAAAGAGAAAAAAACCCTATCAAC
NM_080868.2       AATATCTTCAAAATACTACTGCAATATGGAATCTTAGAAAGAGAAAAAAACCCTATCAAC
                  ************************************************************

HsCD00303228      ATTGTCTTAACAATAGTACTCTACCCTTCGAGAGTAAGAGTAATGGTTGATCGTGAATTG
NM_080868.2       ATTGTCTTAACAATAGTACTCTACCCTTCGAGAGTAAGAGTAATGGTTGATCGTGAATTG
                  ************************************************************

HsCD00303228      GCTGACATCCATGAAGATGCCAAAACATGTTTGGTACTATGTTCCAGAGTGCTTTCTGTC
NM_080868.2       GCTGACATCCATGAAGATGCCAAAACATGTTTGGTACTATGTTCCAGAGTGCTTTCTGTC
                  ************************************************************

HsCD00303228      ATTTCAGTCAAGGAAATAAAGACACAGCTGAGTTTAGGAAGACATCCAATTATTTCAAAT
NM_080868.2       ATTTCAGTCAAGGAAATAAAGACACAGCTGAGTTTAGGAAGACATCCAATTATTTCAAAT
                  ************************************************************

HsCD00303228      TGGTTTGATTACATTCCTTCAACAAGATACAAAGATCCATGTGAACTATTACATCTTTGC
NM_080868.2       TGGTTTGATTACATTCCTTCAACAAGATACAAAGATCCATGTGAACTATTACATCTTTGC
                  ************************************************************

HsCD00303228      AGACTAACCATCAGGAATCAACTATTAACCAACAATATGCTCCCAGATGGAATATTTTCA
NM_080868.2       AGACTAACCATCAGGAATCAACTATTAACCAACAATATGCTCCCAGATGGAATATTTTCA
                  ************************************************************

HsCD00303228      CTTCTAATTCCTGCTCGTCTACAAAACTATCTGAATTTAGAAATCTAC
NM_080868.2       CTTCTAATTCCTGCTCGTCTACAAAACTATCTGAATTTAGAAATCTAA
                  ***********************************************.

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : SOCS_box :
UniProt : Q8WXJ9
HPRD : 10671

Clone

Cloning Information : 473124
HIP Master Clone ID : 394718
Original Clone ID : FLH473124.01L

Organism


TAX_ID : 9606
Species Specific ID: 127247

Genome

Chromosome : 1
Map Location : 1p31.1
Ensembl : ENSG00000154007

Reagents

Labome : ASB17-antibody