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DUS2L (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DUS2
Gene Name:  dihydrouridine synthase 2
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH473951.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: This clone is identical to HsCD00619948
Mutation/
Discrepancy :
No / No
Publications: PMID: 22442237
Title: Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of human dihydrouridine synthase
PMID: 22442237
Title: Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of human dihydrouridine synthase
Authors:
HsCD00303989

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UNAVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1482nts         Open reading frame : 1 to 1482
Reference Sequence Alignment

ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTT
CGGGTAGGGACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTAC
TGTGAGGAGCTGATCGACCTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTC
AGCACAGTGGACTTTGTCGCCCCTGATGATCGAGTTGTCTTCCGCACCTGTGAAAGAGAG
CAGAACAGGGTGGTCTTCCAGATGGGGACTTCAGACGCAGAGCGAGCCCTTGCTGTGGCC
AGGCTTGTAGAAAATGATGTGGCTGGTATTGATGTCAACATGGGCTGTCCAAAACAATAT
TCCACCAAGGGAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC
CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCA
TCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATC
GCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATC
AAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCAC
ATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATG
GTGGCCCGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTG
GAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC
ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTG
CTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTAT
GAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAG
CAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGAC
CGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG
GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCC
TCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA
CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGG
CTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCT
GGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTG
GCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1631
Start on reference sequence 150
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DUS2
Symbol Nomenclature : DUS2
SYNONYM : DUS2L; SMM1; URLC8
Designation : SMM1 homolog|dihydrouridine synthase 2-like, SMM1 homolog|tRNA-dihydrouridine(20) synthase [NAD(P)+]-like|up-regulated in lung cancer protein 8
Full Nomenclature : dihydrouridine synthase 2
GENEID : 54920
SYNONYM : HsCD00619948
HGNC : 26014
MIM : 609707
Vega : OTTHUMG00000137538
Target GenBank: BC006527.2

 

Reference Sequence Alignment

  

HsCD00303989        ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTT
NM_001271763.1      ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTT
NM_017803.4         ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTT
NM_001271762.1      ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTT
                    ************************************************************

HsCD00303989        CGGGTAGGGACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTAC
NM_001271763.1      CGGGTAGGGACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTAC
NM_017803.4         CGGGTAGGGACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTAC
NM_001271762.1      CGGGTAGGGACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTAC
                    ************************************************************

HsCD00303989        TGTGAGGAGCTGATCGACCTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTC
NM_001271763.1      TGTGAGGAGCTGATCGACCTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTC
NM_017803.4         TGTGAGGAGCTGATCGACCTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTC
NM_001271762.1      TGTGAGGAGCTGATCGACCTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTC
                    ************************************************************

HsCD00303989        AGCACAGTGGACTTTGTCGCCCCTGATGATCGAGTTGTCTTCCGCACCTGTGAAAGAGAG
NM_001271763.1      AGCACAGTGGACTTTGTCGCCCCTGATGATCGAGTTGTCTTCCGCACCTGTGAAAGAGAG
NM_017803.4         AGCACAGTGGACTTTGTCGCCCCTGATGATCGAGTTGTCTTCCGCACCTGTGAAAGAGAG
NM_001271762.1      AGCACAGTGGACTTTGTCGCCCCTGATGATCGAGTTGTCTTCCGCACCTGTGAAAGAGAG
                    ************************************************************

HsCD00303989        CAGAACAGGGTGGTCTTCCAGATGGGGACTTCAGACGCAGAGCGAGCCCTTGCTGTGGCC
NM_001271763.1      CAGAACAGGGTGGTCTTCCAGATGG-----------------------------------
NM_017803.4         CAGAACAGGGTGGTCTTCCAGATGGGGACTTCAGACGCAGAGCGAGCCCTTGCTGTGGCC
NM_001271762.1      CAGAACAGGGTGGTCTTCCAGATGGGGACTTCAGACGCAGAGCGAGCCCTTGCTGTGGCC
                    *************************                                   

HsCD00303989        AGGCTTGTAGAAAATGATGTGGCTGGTATTGATGTCAACATGGGCTGTCCAAAACAATAT
NM_001271763.1      ------------------------------------------------------------
NM_017803.4         AGGCTTGTAGAAAATGATGTGGCTGGTATTGATGTCAACATGGGCTGTCCAAAACAATAT
NM_001271762.1      AGGCTTGTAGAAAATGATGTGGCTGGTATTGATGTCAACATGGGCTGTCCAAAACAATAT
                                                                                

HsCD00303989        TCCACCAAGGGAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC
NM_001271763.1      ----------GAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC
NM_017803.4         TCCACCAAGGGAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC
NM_001271762.1      TCCACCAAGGGAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC
                              **************************************************

HsCD00303989        CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCA
NM_001271763.1      CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCA
NM_017803.4         CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCA
NM_001271762.1      CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCA
                    ************************************************************

HsCD00303989        TCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATC
NM_001271763.1      TCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATC
NM_017803.4         TCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATC
NM_001271762.1      TCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATC
                    ************************************************************

HsCD00303989        GCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATC
NM_001271763.1      GCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATC
NM_017803.4         GCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATC
NM_001271762.1      GCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATC
                    ************************************************************

HsCD00303989        AAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCAC
NM_001271763.1      AAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCAC
NM_017803.4         AAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCAC
NM_001271762.1      AAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCAC
                    ************************************************************

HsCD00303989        ATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATG
NM_001271763.1      ATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATG
NM_017803.4         ATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATG
NM_001271762.1      ATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATG
                    ************************************************************

HsCD00303989        GTGGCCCGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTG
NM_001271763.1      GTGGCCCGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTG
NM_017803.4         GTGGCCCGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTG
NM_001271762.1      GTGGCCCGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTG
                    ************************************************************

HsCD00303989        GAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC
NM_001271763.1      GAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC
NM_017803.4         GAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC
NM_001271762.1      GAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC
                    ************************************************************

HsCD00303989        ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTG
NM_001271763.1      ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTG
NM_017803.4         ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTG
NM_001271762.1      ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTG
                    ************************************************************

HsCD00303989        CTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTAT
NM_001271763.1      CTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTAT
NM_017803.4         CTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTAT
NM_001271762.1      CTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTAT
                    ************************************************************

HsCD00303989        GAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAG
NM_001271763.1      GAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAG
NM_017803.4         GAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAG
NM_001271762.1      GAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAG
                    ************************************************************

HsCD00303989        CAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGAC
NM_001271763.1      CAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGAC
NM_017803.4         CAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGAC
NM_001271762.1      CAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGAC
                    ************************************************************

HsCD00303989        CGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG
NM_001271763.1      CGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG
NM_017803.4         CGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG
NM_001271762.1      CGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG
                    ************************************************************

HsCD00303989        GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCC
NM_001271763.1      GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCC
NM_017803.4         GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCC
NM_001271762.1      GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCC
                    ************************************************************

HsCD00303989        TCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA
NM_001271763.1      TCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA
NM_017803.4         TCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA
NM_001271762.1      TCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA
                    ************************************************************

HsCD00303989        CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGG
NM_001271763.1      CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGG
NM_017803.4         CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGG
NM_001271762.1      CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGG
                    ************************************************************

HsCD00303989        CTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCT
NM_001271763.1      CTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCT
NM_017803.4         CTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCT
NM_001271762.1      CTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCT
                    ************************************************************

HsCD00303989        GGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTG
NM_001271763.1      GGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTG
NM_017803.4         GGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTG
NM_001271762.1      GGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTG
                    ************************************************************

HsCD00303989        GCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTAC
NM_001271763.1      GCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTGA
NM_017803.4         GCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTGA
NM_001271762.1      GCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTGA
                    ****************************************..

Protein

SMART domain : DSRM : Double-stranded RNA binding motif
UniProt : Q9NX74
HPRD : 08624

Clone

Cloning Information : 473951
HIP Master Clone ID : 395006
Original Clone ID : FLH473951.01L

Organism


TAX_ID : 9606
Species Specific ID: 54920

Genome

Chromosome : 16
Map Location : 16q22.1
Ensembl : ENSG00000167264

Reagents

Labome : DUS2L-antibody