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U2AF1 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  U2AF1
Gene Name:  U2 small nuclear RNA auxiliary factor 1
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH473571.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00304161

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 504nts         Open reading frame : 1 to 504

ATGCAGGAACACTATGATGAGTTTTTTGAGGAGGTTTTTACAGAAATGGAGGAGAAGTAT
AGGGAAGTAGAGGAGATGAACGTCTGTGACAACCTGGGAGACCACCTGGTGGGGAACGTG
TACGTCAAGTTTCGCCGTGAGGAAGATGCGGAAAAGGCTGTGATTGACTTGAATAACCGT
TGGTTTAATGGACAGCCGATCCACGCCGAGCTGTCACCCGTGACGGACTTCAGAGAAGCC
TGCTGCCGTCAGTATGAGATGGGAGAATGCACACGAGGCGGCTTCTGCAACTTCATGCAT
TTGAAGCCCATTTCCAGAGAGCTGCGGCGGGAGCTGTATGGCCGCCGTCGCAAGAAGCAT
AGATCAAGATCCCGATCCCGGGAGCGTCGTTCTCGGTCTAGAGACCGTGGTCGTGGCGGT
GGCGGTGGCGGTGGTGGAGGTGGCGGCGGACGGGAGCGTGACAGGAGGCGGTCGAGAGAT
CGTGAAAGATCTGGGCGATTCTGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 820
Start on reference sequence 317
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : U2AF1
Symbol Nomenclature : U2AF1
SYNONYM : FP793; RN; RNU2AF1; U2AF35; U2AFBP
Designation : U2 small nuclear RNA auxillary factor 1|U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit|U2 snRNP auxiliary factor small subunit|U2(RNU2) small nuclear RNA auxiliary factor binding protein|splicing factor U2AF 35 kDa subunit|splicing factor U2AF 35kDa subunit
Full Nomenclature : U2 small nuclear RNA auxiliary factor 1
GENEID : 7307
HGNC : 12453
MIM : 191317
Vega : OTTHUMG00000086836
Target GenBank: BC005915.1

 

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : Transport of Mature Transcript to Cytoplasm
Reactome : Transport of Mature mRNA derived from an Intron-Containing Transcript
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM_1 : RNA recognition motif
SMART domain : ZnF_C3H1 : zinc finger
UniProt : Q7Z780
UniProt : Q701P4
UniProt : Q71RF1
UniProt : Q01081
HPRD : 01871

Clone

Cloning Information : 473571
HIP Master Clone ID : 394457
Original Clone ID : FLH473571.01L

Organism


TAX_ID : 9606
Species Specific ID: 7307

Genome

Chromosome : 21
Map Location : 21q22.3
Ensembl : ENSG00000160201

Reagents

Labome : U2AF1-antibody