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Detailed Vector Information: p283

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: p283              
Source: Midwest Center for Structural Genomics
Description: Bacterial (E. coli) expression vector, adds His tag, C-terminal TEV cleavage site fusion; kanamycin resistance; restriction enzyme cloning.
Comments: None
Publications: None
Authors: PSI
Midwest Center for Structural Genomics
EvNO00304412

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AVAILABLE
Academic and non-profit labs
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
       DyNA Vector Map

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Vector Name: p283

p283 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector, adds His tag, C-terminal TEV cleavage site fusion; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5282
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1421 2103
promoter T7 T7 promoter 4984 5003
repressor binding site lacO lacO regulatory sequence 5003 5025
repressor protein gene lacI lacI coding region 3518 4597
selectable marker KanR kanamycin resistance gene 1375 563
ssDNA origin f1 ori f1 origin 17 457
trxn termination sequence T7 term T7 termination sequence 5211 5257

 

Species Specific ID: None
Target GenBank: None