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Detailed Vector Information: pNYCOMPS-TH-ccdB (C-term.)

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pNYCOMPS-TH-ccdB (C-term.)              
Source: New York Consortium on Membrane Protein Structure
Description: Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning.
Comments: None
Publications: PMID: 20394823
Title: High-throughput expression and purification of membrane proteins.
PMID: 24510647
Title: High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli.
Authors: PSI
New York Consortium on Membrane Protein Structure
EvNO00304420

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No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
       DyNA Vector Map

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Vector Name: pNYCOMPS-TH-ccdB (C-term.)

pNYCOMPS-TH-ccdBC-term. in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning.
Comments: Requires growth in ccdB survival strain
Size (bp): 5936
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI
New York Consortium on Membrane Protein Structure
Publications: PMID: 20394823
Title: High-throughput expression and purification of membrane proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
death cassette ccdB ccdB death cassette (on complementary strand). requires growth in ccdB survival strain bacteria 5076 5381
ligation independent cloning 3'LIC 3' LIC overhang 5721 5737
ligation independent cloning 5'LIC 5' LIC overhang 5038 5053
primer T7 term T7 reverse sequencing primer 5853 5871
promoter T7 T7 promoter 4984 5000
protease cleavage site TEV TEV protease cleavage site 5723 5740
repressor protein gene LacI Lac repressor protein 3515 4474
ribosome binding site RBS Ribosomal binding site 5043 5048
selectable marker KanR Kanamycin resistance 560 1375
tag His C-terminal 10XHis tag 5741 5770
trxn regulatory element lac operator lac operator 5003 5025

 

Species Specific ID: None
Target GenBank: None