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P2RY13 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  P2RY13
Gene Name:  purinergic receptor P2Y, G-protein coupled, 13
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH272076.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: BMS
Description: None
Comments: Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.
Mutation/
Discrepancy :
Unknown / Unknown
Publications: None
Authors: Bristol-Myers Squibb
HsCD00304979

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1002nts         Open reading frame : 1 to 1002
Reference Sequence Alignment

ATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACTCGG
ATAGTACAGCTGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTGCTG
AATACTTTGGCTCTGTGGGTGTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATCTAC
CTCAAAAACACTTTGGTGGCCGACTTGATAATGACACTCATGCTTCCTTTCAAAATCCTC
TCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTGTGTGTCGTTTTTCTTCGGTG
ATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGCCTTTGACAGA
TTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCAAAA
ACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATACGATCTTG
AGCAACAAGGAAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCTCTG
GGGCTGAAATGGCATCAAATGGTAAATAACATATGCCAGTTTATTTTCTGGACTGTTTTT
ATCCTAATGCTTGTGTTTTATGTGGTTATTGCAAAAAAAGTATATGATTCTTATAGAAAG
TCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAGGCAAAGTATTTGTTGTCGTG
GCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATATACTCACAGT
CAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAAACA
ACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTATGT
AAAAAATTCACAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGCCAA
GAAAATCATAGCAGTCAGACAGACAACATAACCTTAGGCTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1084
Start on reference sequence 83
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : P2RY13
Symbol Nomenclature : P2RY13
SYNONYM : GPCR1; GPR86; GPR94; P2Y13; SP174
Designation : G protein-coupled receptor 86|G-protein coupled receptor 86|G-protein coupled receptor 94|P2Y purinoceptor 13
Full Nomenclature : purinergic receptor P2Y, G-protein coupled, 13
GENEID : 53829
Locus Tag : FKSG77
HGNC : 4537
MIM : 606380
Vega : OTTHUMG00000155745
Target GenBank: NM_176894

 

Reference Sequence Alignment

  

HsCD00304979      ------------------------------------------------------------
NM_176894.2       ATGACTGCCGCCATAAGAAGACAGAGAGAACTGAGTATCCTCCCAAAGGTGACACTGGAA
                                                                              

HsCD00304979      ---ATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACT
NM_176894.2       GCAATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACT
                     *********************************************************

HsCD00304979      CGGATAGTACAGCTGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTG
NM_176894.2       CGGATAGTACAGCTGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTG
                  ************************************************************

HsCD00304979      CTGAATACTTTGGCTCTGTGGGTGTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATC
NM_176894.2       CTGAATACTTTGGCTCTGTGGGTGTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATC
                  ************************************************************

HsCD00304979      TACCTCAAAAACACTTTGGTGGCCGACTTGATAATGACACTCATGCTTCCTTTCAAAATC
NM_176894.2       TACCTCAAAAACACTTTGGTGGCCGACTTGATAATGACACTCATGCTTCCTTTCAAAATC
                  ************************************************************

HsCD00304979      CTCTCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTGTGTGTCGTTTTTCTTCG
NM_176894.2       CTCTCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTGTGTGTCGTTTTTCTTCG
                  ************************************************************

HsCD00304979      GTGATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGCCTTTGAC
NM_176894.2       GTGATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGCCTTTGAC
                  ************************************************************

HsCD00304979      AGATTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCA
NM_176894.2       AGATTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCA
                  ************************************************************

HsCD00304979      AAAACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATACGATC
NM_176894.2       AAAACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATACGATC
                  ************************************************************

HsCD00304979      TTGAGCAACAAGGAAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCT
NM_176894.2       TTGAGCAACAAGGAAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCT
                  ************************************************************

HsCD00304979      CTGGGGCTGAAATGGCATCAAATGGTAAATAACATATGCCAGTTTATTTTCTGGACTGTT
NM_176894.2       CTGGGGCTGAAATGGCATCAAATGGTAAATAACATATGCCAGTTTATTTTCTGGACTGTT
                  ************************************************************

HsCD00304979      TTTATCCTAATGCTTGTGTTTTATGTGGTTATTGCAAAAAAAGTATATGATTCTTATAGA
NM_176894.2       TTTATCCTAATGCTTGTGTTTTATGTGGTTATTGCAAAAAAAGTATATGATTCTTATAGA
                  ************************************************************

HsCD00304979      AAGTCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAGGCAAAGTATTTGTTGTC
NM_176894.2       AAGTCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAGGCAAAGTATTTGTTGTC
                  ************************************************************

HsCD00304979      GTGGCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATATACTCAC
NM_176894.2       GTGGCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATATACTCAC
                  ************************************************************

HsCD00304979      AGTCAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAA
NM_176894.2       AGTCAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAA
                  ************************************************************

HsCD00304979      ACAACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTA
NM_176894.2       ACAACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTA
                  ************************************************************

HsCD00304979      TGTAAAAAATTCACAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGC
NM_176894.2       TGTAAAAAATTCACAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGC
                  ************************************************************

HsCD00304979      CAAGAAAATCATAGCAGTCAGACAGACAACATAACCTTAGGCTGA
NM_176894.2       CAAGAAAATCATAGCAGTCAGACAGACAACATAACCTTAGGCTGA
                  *********************************************

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : GPCRs, Other

Protein

HPRD : 16210

Clone

Cloning Information : 272076
HIP Master Clone ID : 202439
Original Clone ID : FLH272076.01L

Organism


TAX_ID : 9606
Species Specific ID: 53829

Genome

Chromosome : 3
Map Location : 3q24
Ensembl : ENSG00000181631