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CALCRL (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CALCRL
Gene Name:  calcitonin receptor-like
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH272207.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: BMS
Description: None
Comments: Clone insert was fully sequence-verified by the clone submitter but the sequence is not available. May contain incorrect sequence.
Mutation/
Discrepancy :
Unknown / Unknown
Publications: None
Authors: Bristol-Myers Squibb
HsCD00305110

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1386nts         Open reading frame : 1 to 1386
Reference Sequence Alignment

ATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCTCTTGCCTTTTTTTATGATTCTTGTT
ACAGCAGAATTAGAAGAGAGTCCTGAGGACTCAATTCAGTTGGGAGTTACTAGAAATAAA
ATCATGACAGCTCAATATGAATGTTACCAAAAGATTATGCAAGACCCCATTCAACAAGCA
GAAGGCGTTTACTGCAACAGAACCTGGGATGGATGGCTCTGCTGGAACGATGTTGCAGCA
GGAACTGAATCAATGCAGCTCTGCCCTGATTACTTTCAGGACTTTGATCCATCAGAAAAA
GTTACAAAGATCTGTGACCAAGATGGAAACTGGTTTAGACATCCAGCAAGCAACAGAACA
TGGACAAATTATACCCAGTGTAATGTTAACACCCACGAGAAAGTGAAGACTGCACTAAAT
TTGTTTTACCTGACCATAATTGGACACGGATTGTCTATTGCATCACTGCTTATCTCGCTT
GGCATATTCTTTTATTTCAAGAGCCTAAGTTGCCAAAGGATTACCTTACACAAAAATCTG
TTCTTCTCATTTGTTTGTAACTCTGTTGTAACAATCATTCACCTCACTGCAGTGGCCAAC
AACCAGGCCTTAGTAGCCACAAATCCTGTTAGTTGCAAAGTGTCCCAGTTCATTCATCTT
TACCTGATGGGCTGTAATTACTTTTGGATGCTCTGTGAAGGCATTTACCTACACACACTC
ATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTTAATGTGGTATTATTTTCTTGGCTGG
GGATTTCCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGCTTATATTACAATGAC
AATTGCTGGATCAGTTCTGATACCCATCTCCTCTACATTATCCATGGCCCAATTTGTGCT
GCTTTACTGGTGAATCTTTTTTTCTTGTTAAATATTGTACGCGTTCTCATCACCAAGTTA
AAAGTTACACACCAAGCGGAATCCAATCTGTACATGAAAGCTGTGAGAGCTACTCTTATC
TTGGTGCCATTGCTTGGCATTGAATTTGTGCTGATTCCATGGCGACCTGAAGGAAAGATT
GCAGAGGAGGTATATGACTACATCATGCACATCCTTATGCACTTCCAGGGTCTTTTGGTC
TCTACCATTTTCTGCTTCTTTAATGGAGAGGTTCAAGCAATTCTGAGAAGAAACTGGAAT
CAATACAAAATCCAATTTGGAAACAGCTTTTCCAACTCAGAAGCTCTTCGTAGTGCGTCT
TACACAGTGTCAACAATCAGTGATGGTCCAGGTTATAGTCATGACTGTCCTAGTGAACAC
TTAAATGGAAAAAGCATCCATGATATTGAAAATGTTCTCTTAAAACCAGAAAATTTATAT
AATTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1934
Start on reference sequence 549
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : CALCRL
Symbol Nomenclature : CALCRL
SYNONYM : CGRPR; CRLR
Designation : CGRP type 1 receptor|calcitonin gene-related peptide type 1 receptor|calcitonin receptor-like receptor
Full Nomenclature : calcitonin receptor-like
GENEID : 10203
HGNC : 16709
MIM : 114190
Vega : OTTHUMG00000132636
Target GenBank: NM_005795

 

Reference Sequence Alignment

  

HsCD00305110        ATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCTCTTGCCTTTTTTTATGATTCTTGTT
NM_005795.5         ATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCTCTTGCCTTTTTTTATGATTCTTGTT
NM_001271751.1      ATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCTCTTGCCTTTTTTTATGATTCTTGTT
                    ************************************************************

HsCD00305110        ACAGCAGAATTAGAAGAGAGTCCTGAGGACTCAATTCAGTTGGGAGTTACTAGAAATAAA
NM_005795.5         ACAGCAGAATTAGAAGAGAGTCCTGAGGACTCAATTCAGTTGGGAGTTACTAGAAATAAA
NM_001271751.1      ACAGCAGAATTAGAAGAGAGTCCTGAGGACTCAATTCAGTTGGGAGTTACTAGAAATAAA
                    ************************************************************

HsCD00305110        ATCATGACAGCTCAATATGAATGTTACCAAAAGATTATGCAAGACCCCATTCAACAAGCA
NM_005795.5         ATCATGACAGCTCAATATGAATGTTACCAAAAGATTATGCAAGACCCCATTCAACAAGCA
NM_001271751.1      ATCATGACAGCTCAATATGAATGTTACCAAAAGATTATGCAAGACCCCATTCAACAAGCA
                    ************************************************************

HsCD00305110        GAAGGCGTTTACTGCAACAGAACCTGGGATGGATGGCTCTGCTGGAACGATGTTGCAGCA
NM_005795.5         GAAGGCGTTTACTGCAACAGAACCTGGGATGGATGGCTCTGCTGGAACGATGTTGCAGCA
NM_001271751.1      GAAGGCGTTTACTGCAACAGAACCTGGGATGGATGGCTCTGCTGGAACGATGTTGCAGCA
                    ************************************************************

HsCD00305110        GGAACTGAATCAATGCAGCTCTGCCCTGATTACTTTCAGGACTTTGATCCATCAGAAAAA
NM_005795.5         GGAACTGAATCAATGCAGCTCTGCCCTGATTACTTTCAGGACTTTGATCCATCAGAAAAA
NM_001271751.1      GGAACTGAATCAATGCAGCTCTGCCCTGATTACTTTCAGGACTTTGATCCATCAGAAAAA
                    ************************************************************

HsCD00305110        GTTACAAAGATCTGTGACCAAGATGGAAACTGGTTTAGACATCCAGCAAGCAACAGAACA
NM_005795.5         GTTACAAAGATCTGTGACCAAGATGGAAACTGGTTTAGACATCCAGCAAGCAACAGAACA
NM_001271751.1      GTTACAAAGATCTGTGACCAAGATGGAAACTGGTTTAGACATCCAGCAAGCAACAGAACA
                    ************************************************************

HsCD00305110        TGGACAAATTATACCCAGTGTAATGTTAACACCCACGAGAAAGTGAAGACTGCACTAAAT
NM_005795.5         TGGACAAATTATACCCAGTGTAATGTTAACACCCACGAGAAAGTGAAGACTGCACTAAAT
NM_001271751.1      TGGACAAATTATACCCAGTGTAATGTTAACACCCACGAGAAAGTGAAGACTGCACTAAAT
                    ************************************************************

HsCD00305110        TTGTTTTACCTGACCATAATTGGACACGGATTGTCTATTGCATCACTGCTTATCTCGCTT
NM_005795.5         TTGTTTTACCTGACCATAATTGGACACGGATTGTCTATTGCATCACTGCTTATCTCGCTT
NM_001271751.1      TTGTTTTACCTGACCATAATTGGACACGGATTGTCTATTGCATCACTGCTTATCTCGCTT
                    ************************************************************

HsCD00305110        GGCATATTCTTTTATTTCAAGAGCCTAAGTTGCCAAAGGATTACCTTACACAAAAATCTG
NM_005795.5         GGCATATTCTTTTATTTCAAGAGCCTAAGTTGCCAAAGGATTACCTTACACAAAAATCTG
NM_001271751.1      GGCATATTCTTTTATTTCAAGAGCCTAAGTTGCCAAAGGATTACCTTACACAAAAATCTG
                    ************************************************************

HsCD00305110        TTCTTCTCATTTGTTTGTAACTCTGTTGTAACAATCATTCACCTCACTGCAGTGGCCAAC
NM_005795.5         TTCTTCTCATTTGTTTGTAACTCTGTTGTAACAATCATTCACCTCACTGCAGTGGCCAAC
NM_001271751.1      TTCTTCTCATTTGTTTGTAACTCTGTTGTAACAATCATTCACCTCACTGCAGTGGCCAAC
                    ************************************************************

HsCD00305110        AACCAGGCCTTAGTAGCCACAAATCCTGTTAGTTGCAAAGTGTCCCAGTTCATTCATCTT
NM_005795.5         AACCAGGCCTTAGTAGCCACAAATCCTGTTAGTTGCAAAGTGTCCCAGTTCATTCATCTT
NM_001271751.1      AACCAGGCCTTAGTAGCCACAAATCCTGTTAGTTGCAAAGTGTCCCAGTTCATTCATCTT
                    ************************************************************

HsCD00305110        TACCTGATGGGCTGTAATTACTTTTGGATGCTCTGTGAAGGCATTTACCTACACACACTC
NM_005795.5         TACCTGATGGGCTGTAATTACTTTTGGATGCTCTGTGAAGGCATTTACCTACACACACTC
NM_001271751.1      TACCTGATGGGCTGTAATTACTTTTGGATGCTCTGTGAAGGCATTTACCTACACACACTC
                    ************************************************************

HsCD00305110        ATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTTAATGTGGTATTATTTTCTTGGCTGG
NM_005795.5         ATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTTAATGTGGTATTATTTTCTTGGCTGG
NM_001271751.1      ATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTTAATGTGGTATTATTTTCTTGGCTGG
                    ************************************************************

HsCD00305110        GGATTTCCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGCTTATATTACAATGAC
NM_005795.5         GGATTTCCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGCTTATATTACAATGAC
NM_001271751.1      GGATTTCCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGCTTATATTACAATGAC
                    ************************************************************

HsCD00305110        AATTGCTGGATCAGTTCTGATACCCATCTCCTCTACATTATCCATGGCCCAATTTGTGCT
NM_005795.5         AATTGCTGGATCAGTTCTGATACCCATCTCCTCTACATTATCCATGGCCCAATTTGTGCT
NM_001271751.1      AATTGCTGGATCAGTTCTGATACCCATCTCCTCTACATTATCCATGGCCCAATTTGTGCT
                    ************************************************************

HsCD00305110        GCTTTACTGGTGAATCTTTTTTTCTTGTTAAATATTGTACGCGTTCTCATCACCAAGTTA
NM_005795.5         GCTTTACTGGTGAATCTTTTTTTCTTGTTAAATATTGTACGCGTTCTCATCACCAAGTTA
NM_001271751.1      GCTTTACTGGTGAATCTTTTTTTCTTGTTAAATATTGTACGCGTTCTCATCACCAAGTTA
                    ************************************************************

HsCD00305110        AAAGTTACACACCAAGCGGAATCCAATCTGTACATGAAAGCTGTGAGAGCTACTCTTATC
NM_005795.5         AAAGTTACACACCAAGCGGAATCCAATCTGTACATGAAAGCTGTGAGAGCTACTCTTATC
NM_001271751.1      AAAGTTACACACCAAGCGGAATCCAATCTGTACATGAAAGCTGTGAGAGCTACTCTTATC
                    ************************************************************

HsCD00305110        TTGGTGCCATTGCTTGGCATTGAATTTGTGCTGATTCCATGGCGACCTGAAGGAAAGATT
NM_005795.5         TTGGTGCCATTGCTTGGCATTGAATTTGTGCTGATTCCATGGCGACCTGAAGGAAAGATT
NM_001271751.1      TTGGTGCCATTGCTTGGCATTGAATTTGTGCTGATTCCATGGCGACCTGAAGGAAAGATT
                    ************************************************************

HsCD00305110        GCAGAGGAGGTATATGACTACATCATGCACATCCTTATGCACTTCCAGGGTCTTTTGGTC
NM_005795.5         GCAGAGGAGGTATATGACTACATCATGCACATCCTTATGCACTTCCAGGGTCTTTTGGTC
NM_001271751.1      GCAGAGGAGGTATATGACTACATCATGCACATCCTTATGCACTTCCAGGGTCTTTTGGTC
                    ************************************************************

HsCD00305110        TCTACCATTTTCTGCTTCTTTAATGGAGAGGTTCAAGCAATTCTGAGAAGAAACTGGAAT
NM_005795.5         TCTACCATTTTCTGCTTCTTTAATGGAGAGGTTCAAGCAATTCTGAGAAGAAACTGGAAT
NM_001271751.1      TCTACCATTTTCTGCTTCTTTAATGGAGAGGTTCAAGCAATTCTGAGAAGAAACTGGAAT
                    ************************************************************

HsCD00305110        CAATACAAAATCCAATTTGGAAACAGCTTTTCCAACTCAGAAGCTCTTCGTAGTGCGTCT
NM_005795.5         CAATACAAAATCCAATTTGGAAACAGCTTTTCCAACTCAGAAGCTCTTCGTAGTGCGTCT
NM_001271751.1      CAATACAAAATCCAATTTGGAAACAGCTTTTCCAACTCAGAAGCTCTTCGTAGTGCGTCT
                    ************************************************************

HsCD00305110        TACACAGTGTCAACAATCAGTGATGGTCCAGGTTATAGTCATGACTGTCCTAGTGAACAC
NM_005795.5         TACACAGTGTCAACAATCAGTGATGGTCCAGGTTATAGTCATGACTGTCCTAGTGAACAC
NM_001271751.1      TACACAGTGTCAACAATCAGTGATGGTCCAGGTTATAGTCATGACTGTCCTAGTGAACAC
                    ************************************************************

HsCD00305110        TTAAATGGAAAAAGCATCCATGATATTGAAAATGTTCTCTTAAAACCAGAAAATTTATAT
NM_005795.5         TTAAATGGAAAAAGCATCCATGATATTGAAAATGTTCTCTTAAAACCAGAAAATTTATAT
NM_001271751.1      TTAAATGGAAAAAGCATCCATGATATTGAAAATGTTCTCTTAAAACCAGAAAATTTATAT
                    ************************************************************

HsCD00305110        AATTGA
NM_005795.5         AATTGA
NM_001271751.1      AATTGA
                    ******

Pathways

Reactome : Calcitonin-like ligand receptors
Reactome : Class B/2 (Secretin family receptors)
Reactome : G alpha (s) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class B Secretin-like

Protein

SMART domain : HormR : Domain present in hormone receptors
HPRD : 11809

Clone

Cloning Information : 272207
HIP Master Clone ID : 202570
Original Clone ID : FLH272207.01L

Organism


TAX_ID : 9606
Species Specific ID: 10203

Genome

Chromosome : 2
Map Location : 2q32.1
Ensembl : ENSG00000064989