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FGF13 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF13
Gene Name:  fibroblast growth factor 13
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH272696.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.
Mutation/
Discrepancy :
Unknown / Unknown
Publications: None
Authors:
HsCD00305410

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 738nts         Open reading frame : 1 to 738
Reference Sequence Alignment

ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGAG
AAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAAA
AACAAGTTAAATGTCTTTTCCCGGGTCAAACTCTTCGGCTCCAAGAAGAGGCGCAGAAGA
AGACCAGAGCCTCAGCTTAAGGGTATAGTTACCAAGCTATACAGCCGACAAGGCTACCAC
TTGCAGCTGCAGGCGGATGGAACCATTGATGGCACCAAAGATGAGGACAGCACTTACACT
CTGTTTAACCTCATCCCTGTGGGTCTGCGAGTGGTGGCTATCCAAGGAGTTCAAACCAAG
CTGTACTTGGCAATGAACAGTGAGGGATACTTGTACACCTCGGAACTTTTCACACCTGAG
TGCAAATTCAAAGAATCAGTGTTTGAAAATTATTATGTGACATATTCATCAATGATATAC
CGTCAGCAGCAGTCAGGCCGAGGGTGGTATCTGGGTCTGAACAAAGAAGGAGAGATCATG
AAAGGCAACCATGTGAAGAAGAACAAGCCTGCAGCTCATTTTCTGCCTAAACCACTGAAA
GTGGCCATGTACAAGGAGCCATCACTGCACGATCTCACGGAGTTCTCCCGATCTGGAAGC
GGGACCCCAACCAAGAGCAGAAGTGTCTCTGGCGTGCTGAACGGAGGCAAATCCATGAGC
CACAATGAATCAACGTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 968
Start on reference sequence 231
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF13
Symbol Nomenclature : FGF13
SYNONYM : FGF-13; FGF2; FHF-2; FHF2
Designation : fibroblast growth factor homologous factor 2
Full Nomenclature : fibroblast growth factor 13
GENEID : 2258
Locus Tag : RP6-27P15.3
HGNC : 3670
MIM : 300070
Vega : OTTHUMG00000022532
Target GenBank: NM_004114

 

Reference Sequence Alignment

  

HsCD00305410        -ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGA
NM_001139501.1      ------------------------------------------------------------
XM_005262399.1      ATGAGTGGAAAGGTGACCAAGCCCAAAGAGGAGAAAGATGCTTCTAAGGTTCTGGATGAC
NM_004114.3         -ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGA
NM_001139502.1      ------------------------------------------------------------
NM_001139500.1      ATGAGTGGAAAGGTGACCAAGCCCAAAGAGGAGAAAGATGCTTCTAAGGTTCTGGATGAC
                                                                                

HsCD00305410        GAAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAA
NM_001139501.1      ---------------------------ATGTTACGACAAGATTCCATCCAATCTGCGGAA
XM_005262399.1      GCCCCCCCTGGCACACAGGAATACATTATGTTACGACAAGATTCCATCCAATCTGCGGAA
NM_004114.3         GAAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAA
NM_001139502.1      ---------------------------ATGTTACGACAAGATTCCATCCAATCTGCGGAA
NM_001139500.1      GCCCCCCCTGGCACACAGGAATACATTATGTTACGACAAGATTCCATCCAATCTGCGGAA
                                               .:*  .*..***..  ...:***.     . **

HsCD00305410        AAACAAGTTAAATG-----------------------------TCTTTTCCCGGGTCAAA
NM_001139501.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
XM_005262399.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
NM_004114.3         AAACAAGTTAAATG-----------------------------TCTTTTCCCGGGTCAAA
NM_001139502.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
NM_001139500.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
                    ::*.*..::*** .                             **** * * *    .:.

HsCD00305410        CTCTTCGGCTCCAAGAAGAGGCGCAGAAGAAGACCAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139501.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
XM_005262399.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
NM_004114.3         CTCTTCGGCTCCAAGAAGAGGCGCAGAAGAAGACCAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139502.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139500.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
                    .:  :.* . .*.* **...*..** *.... . .*************************

HsCD00305410        ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139501.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
XM_005262399.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_004114.3         ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139502.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139500.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
                    ************************************************************

HsCD00305410        GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139501.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
XM_005262399.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_004114.3         GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139502.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139500.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
                    ************************************************************

HsCD00305410        GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139501.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
XM_005262399.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_004114.3         GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139502.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139500.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
                    ************************************************************

HsCD00305410        TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139501.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
XM_005262399.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_004114.3         TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139502.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139500.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
                    ************************************************************

HsCD00305410        TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139501.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
XM_005262399.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_004114.3         TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139502.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139500.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
                    ************************************************************

HsCD00305410        CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139501.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
XM_005262399.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_004114.3         CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139502.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139500.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
                    ************************************************************

HsCD00305410        GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139501.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
XM_005262399.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_004114.3         GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139502.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139500.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
                    ************************************************************

HsCD00305410        GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139501.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
XM_005262399.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_004114.3         GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139502.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139500.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
                    ************************************************************

HsCD00305410        GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139501.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
XM_005262399.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_004114.3         GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139502.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139500.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
                    ************************************************

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 272696
HIP Master Clone ID : 202468
Original Clone ID : FLH272696.01L

Organism


TAX_ID : 9606
Species Specific ID: 2258

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000129682

Reagents

Labome : FGF13-antibody