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Detailed Vector Information: pMA510

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMA510              
Source: Integrated Center for Structure and Function Innovation
Description: Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).  
Comments: None
Publications: None
Authors:
EvNO00305687

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
       DyNA Vector Map

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Vector Name: pMA510

pMA510 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).  
Comments: Requires special host strain E. coli DB3.1 for growth
Size (bp): 7141
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Integrated Center for Structure and Function Innovation
Mark Arbing
David Eisenberg
PSI
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial operon lac operator Lac operator CDS 5003 5025
bacterial origin ColE ColE1 pBR322 origin 1421 2103
death cassette ccdB ccdB death cassette 6637 3846
phage origin f1 f1 phage origin 29 467
primer T7 term primer T7 terminator reverse sequencing primer 7058 7076
promoter T7 T7 promoter 4984 5000
protease cleavage site TEV TEV protease cleavage site 6208 6228
repressor protein gene LacI LacI repressor gene 3515 4594
ribosome binding site RBS ribosomal binding site 5055 5063
selectable marker KanR kanamycin resistance gene 563 1375
tag MBP N-termal MBP fusion tag 5071 6183
tag His N-terminal 6xHis tag 6190 6207

 

Species Specific ID: None
Target GenBank: None