Integrated Center for Structure and Function Innovation
Description:
Bacterial expression vector with a T7 promoter, N-terminal MBP and C-terminal 6xHis tag-TEV protease site; constitutively expresses TEV protease to produce C-terminal His-tagged protein; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
Comments:
None
Publications:
None
Authors:
EvNO00305688
Price:
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AVAILABLE
No restriction
Special MTA:
PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Bacterial expression vector with a T7 promoter, N-terminal MBP and C-terminal 6xHis tag-TEV protease site; constitutively expresses TEV protease to produce C-terminal His-tagged protein; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
Comments:
Requires special host strain E. coli DB3.1 for growth
Size (bp):
8205
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
David Eisenberg
Mark Arbing
Integrated Center for Structure and Function Innovation
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial operon
lac operator
Lac operator CDS
6034
6058
bacterial origin
ColE
ColE1 pBR322 origin
2084
2084
death cassette
ccdB
ccdB death cassette
7239
8012
gene fragment
TEV cds
TEV protease cds (TEV protease is constitutively expressed from this plasmid allowing in vivo proteolysis of the expressed fusion protien so that only the target with His6 tag is purified)