Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
VfR117-21.1
Keyword:
None
Species:
Aliivibrio fischeri
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
Uniprot: Putative uncharacterized protein
Mutation / Discrepancy:
No / No
Publications:
PMID: 22102225
Title: Solution NMR structure of VF0530 from Vibrio fischeri reveals a nucleic acid-binding function
Authors:
PSI
Northeast Structural Genomics Consortium
VfCD00305952
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 240nts
Open reading frame: 51 to 290

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCATTAA
TTATGACCCAACAAAATAACCCGCTACACGGTATTACATTACAGAAGTTACTTACTGAAT
TGGTAGAACACTATGGCTGGGAAGAGCTAAGTTATATGGTAAACATTAACTGTTTTAAGA
AAGACCCAAGCATTAAATCAAGCCTTAAATTTTTACGTAAAACCGATTGGGCTCGTGAAC
GTGTTGAAAATATTTATCTGAAACTACAGCGCCATAAAGAAAGAAATCAACTCGAGCACC
ACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCC

Protein sequence predicted based on nucleotide sequence

MALIMTQQNNPLHGITLQKLLTELVEHYGWEELSYMVNINCFKKDPSIKSSLKFLRKTDW
ARERVENIYLKLQRHKERNQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 240
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in VfR117
Recommended expression in:
Not Applicable