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Detailed Vector Information: pMYC5-H

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMYC5-H              
Source: Center for Eukaryotic Structural Genomics
Description: Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI).
Comments: None
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Authors: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
EvNO00306566

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  hygromycin
Host Type:  mycobacteria    Marker:  hygromycin
       DyNA Vector Map

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Vector Name: pMYC5-H

pMYC5-H in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Phsp
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI).
Comments: Target gene controlled by Phsp promoter for expression is cloned between NdeI and SbfI restriction sites. Modified from PMYC4H with unique SrfI site.
Size (bp): 5157
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 457 563
bacterial origin ColE ColE1 replication origin (on complementary strand) 1242 1776
mycobacterial origin myc ori mycobacterial origin of replication 1963 3808
promoter Phsp Phsp promoter 77 456
selectable marker HygR hygromycin resistance cassette 4145 5143
tag His C-terminal His tag 500 532
tag myc C-terminal c-myc tag 500 535
trxn termination sequence T4g32 T4g32 transcription terminator 578 598
trxn termination sequence rrnB rrnB T2 transcriptional terminator 42 62

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None