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Detailed Vector Information: pMYC4-S

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMYC4-S              
Source: Center for Eukaryotic Structural Genomics
Description: Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments: None
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Authors: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
EvNO00306568

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  hygromycin
Host Type:  mycobacteria    Marker:  hygromycin
       DyNA Vector Map

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Vector Name: pMYC4-S

pMYC4-S in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Ps-Pimyc
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments: Target gene controlled by Psmyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp): 5115
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 395 521
bacterial origin ColE ColE1 replication origin (on complementary strand) 1200 1734
mycobacterial origin myc ori mycobacterial origin of replication 1321 3766
promoter Psmyc Psmyc promoter 84 393
selectable marker HygR hygromycin resistance cassette 4103 5101
tag His His tag 458 490
tag myc C-terminal c-myc tag 458 493
trxn termination sequence rrnB rrnB T2 transcriptional terminator 49 69
trxn termination sequence T4g32 T4g32 transcription terminator 536 556

 

Species Specific ID: None
Target GenBank: None