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Detailed Vector Information: p4-Ib

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: p4-Ib              
Source: Center for Eukaryotic Structural Genomics
Description: Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
Comments: None
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Authors: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
EvNO00306571

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  hygromycin
Host Type:  mycobacteria    Marker:  hygromycin
       DyNA Vector Map

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Vector Name: p4-Ib

p4-Ib in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  p4
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
Comments: Target gene controlled by Pimyc promoter for expression is cloned between AsiSI and PmeI restriction sites.
Size (bp): 4928
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 220 334
bacterial origin ColE ColE1 replication origin (on complementary strand) 1013 1547
mycobacterial origin myc ori mycobacterial origin of replication 1734 3579
promoter Pimyc Pimyc promoter 84 219
selectable marker HygR hygromycin resistance cassette 3916 4914
tag His His tag 271 303
tag myc C-terminal c-myc tag 271 306
trxn termination sequence T4g32 T4g32 transcription terminator 349 369
trxn termination sequence rrnB rrnB T2 transcriptional terminator 49 69

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None