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Detailed Vector Information: p9T-S

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: p9T-S              
Source: Center for Eukaryotic Structural Genomics
Description: Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI).
Comments: None
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Authors: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
EvNO00306625

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  hygromycin
Host Type:  mycobacteria    Marker:  hygromycin
       DyNA Vector Map

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Vector Name: p9T-S

p9T-S in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  PtetO
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI).
Comments: Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Psmyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp): 8203
Parent Vector: None
Properties: multiple cloning site
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 376 412
bacterial origin ColE ColE1 replication origin (on complementary strand) 6734 7267
gene fragment 83170 C. merolae 10D unknown heme-binding protiein; used as a 3' stablibier of gene expression 427 879
mycobacterial origin myc ori mycobacterial origin of replication 1421 3895
promoter PtetO UV15 with two tet operators; tetracycline-inducible promoter 72 370
promoter Psmyc Psmyc promoters; located a nucleotides 4247-4563 and 692-1264 4247 4563
repressor protein gene TET tetR(B) Tet Repressor class B 4590 5213
selectable marker HygR hygromycin resistance cassette (on complementary strand) 5320 6318
trxn termination sequence T4g32 2 T4g32 transcription terminator; located from nucleotdies 1312-1330 and5249-5267 1312 1330
trxn termination sequence rrnB 3 rrnBT2 transcriptional terminators; located from nucleotides 24-43, 913-932 and 4207-4226 24 43

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None