Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
None
Publications:
None
Authors:
Center for Eukaryotic Structural Genomics
PSI
EvNO00306665
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Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain