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Detailed Vector Information: pVP91A

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pVP91A              
Source: Center for Eukaryotic Structural Genomics
Description: Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning.
Comments: None
Publications: PMID: 
Title: "Mechanistic and structural studies of the non-heme iron oxygenase enzymes protocatechuate 3,4-dioxygenase, CmlA, and CmlI"
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors: PSI
Center for Eukaryotic Structural Genomics
EvNO00306666

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
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Vector Name: pVP91A

pVP91A in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning.
Comments: Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain.
Size (bp): 8538
Parent Vector: None
Properties: bacterial expression, with tag/fusion/marker
Author Name: PSI
Center for Eukaryotic Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4359 5586
gene fragment sacB sucrase gene from B. subtillis 496 1920
gene fragment CAT chloramphenicol acetyltransferase gene 2094 2748
promoter T5 T5 promoter region 122 202
repressor protein gene LacI LacI-wt coding region (on complementary strand) 3189 4280
selectable marker AmpR ampicillin resistance cassette (on complementary strand) 5593 6452
tag His N-terminal 8xHis tag 231 262
trxn termination sequence T7 term T7 terminator reverse (on complementary strand) 2808 2825
trxn termination sequence rrnB rrnB transcription termination 3005 3101

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None