Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pEU-HSBC
Source:
Center for Eukaryotic Structural Genomics
Description:
In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
None
Publications:
PMID: 23301161
Title: Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes
PMID: 19892197
Title: Cell-free translation of integral membrane proteins into unilamelar liposomes.
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Title: Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes
PMID: 19892197
Title: Cell-free translation of integral membrane proteins into unilamelar liposomes.
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors:
Center for Eukaryotic Structural Genomics
PSI
PSI
EvNO00306667
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pEU-HSBC
Synonyms:
''
Sequencing Primer:
Forward:
His2 Forward
Reverse:
T7 terminator
Description:
In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp):
6336
Parent Vector:
None
Properties:
cell-free expression, in vitro transcription, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
PSI
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
gene fragment
sacB
sucrase gene from B. subtillis
790
2209
gene fragment
CAT
chloramphenicol acetyltransferase gene
2381
3037
primer
His2 forward
His2 forward sequencing primer
424
449
primer
T7 reverse
T7 terminator reverse sequencing primer
3250
3270
promoter
SP6
SP6 promoter
373
392
selectable marker
AmpR
ampicillin resistance cassette (on complementary strand)
4956
5816
tag
His
N-terminal 6xHis tag
527
544
trln enhancer
trln enhancer
TMV-Omega translational enhancer
398
520
