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Detailed Vector Information: pEU-HSBC

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pEU-HSBC              
Source: Center for Eukaryotic Structural Genomics
Description: In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments: None
Publications: PMID: 23301161
Title: Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes
PMID: 19892197
Title: Cell-free translation of integral membrane proteins into unilamelar liposomes.
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
Authors: PSI
Center for Eukaryotic Structural Genomics
EvNO00306667

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pEU-HSBC

pEU-HSBC in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  His2 Forward
Reverse:  T7 terminator
Description: In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments: Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp): 6336
Parent Vector: None
Properties: cell-free expression, in vitro transcription, with tag/fusion/marker
Author Name: PSI
Center for Eukaryotic Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment CAT chloramphenicol acetyltransferase gene 2381 3037
gene fragment sacB sucrase gene from B. subtillis 790 2209
primer T7 reverse T7 terminator reverse sequencing primer 3250 3270
primer His2 forward His2 forward sequencing primer 424 449
promoter SP6 SP6 promoter 373 392
selectable marker AmpR ampicillin resistance cassette (on complementary strand) 4956 5816
tag His N-terminal 6xHis tag 527 544
trln enhancer trln enhancer TMV-Omega translational enhancer 398 520

 

Species Specific ID: None
Target GenBank: None