DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pVP65K

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pVP65K              
Source: Center for Eukaryotic Structural Genomics
Description: Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments: None
Publications: PMID: 18988018
Title: Flexi vector cloning.
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors: Center for Eukaryotic Structural Genomics
University of Wisconsin
EvNO00306668

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI,Promega

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : BR610 T1 phage resistant DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pVP65K

pVP65K in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  Unknown
Description: Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments: Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp): 7174
Parent Vector: None
Properties: bacterial expression
Author Name: PSI
Center for Eukaryotic Structural Genomics
University of Wisconsin
Publications: PMID: 18988018
Title: Flexi vector cloning.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4977 6204
gene fragment BarCat BarCat cassette including Barnase 2245 3376
gene fragment TVMV cds TVMV gene 139 849
primer T7 term T7 terminator reverse primer 3426 3443
primer MBP forward MBP forward primer 2071 2091
promoter T5 T5 promoter region 952 1032
promoter LacIq LacIq-wt promoter 4899 4967
promoter TET TET promoter 873 946
protease cleavage site TVMV TVMV protease cleavage site 2189 2209
repressor binding site LacI LacI coding region 3807 4898
selectable marker KanR Kanamycin resistance gene 6213 7174
tag His 8xHis tag 2210 2234
tag MBP N-terminal maltose binding protein tag 1061 2165
trxn termination sequence rrnB rrnB transcription terminator 15 111

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None