Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol:  HSCB
Gene Name:  HscB mitochondrial iron-sulfur cluster co-chaperone
Original Clone ID:
2189.1.31_GO.91296
Keyword:
partial cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Parent TargetDB: GO.37267; His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
PMID: 18713742
Title: Structure of human J-type co-chaperone HscB reveals a tetracysteine metal-binding domain.
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00310288
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 642nts
Open reading frame: 28 to 669

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCTGCGTCGCAG
GCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGCGGCCCATGGGGCCCCGGGCGGGAG
GACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTAC
TTCAGCCTTATGGACTGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGG
TACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAA
AAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCC
CCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACA
GATTATGAAATGGACAGGCAATTCCTCATAGAAATAATGGAAATCAATGAAAAACTCGCA
GAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAA
GAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAA
ATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGATT
CCCCTTTAATAGGACCCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSAASQAGSNYPRCWNCGGPWGPGREDRFFCPQCRALQAPDPTRDYFSLMDCNRS
FRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYKTLLAPLSRGLYLL
KLHGIEIPERTDYEMDRQFLIEIMEINEKLAEAESEAAMKEIESIVKAKQKEFTDNVSSA
FEQDDFEEAKEILTKMRYFSNIEEKIKLKKIPL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.91296
Recommended expression in:
Not Applicable