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EMD (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EMD
Gene Name:  emerin
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH024062.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003111

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 765nts         Open reading frame : 1 to 765
Reference Sequence Alignment

ATGGACAACTACGCAGATCTTTCGGATACCGAGCTGACCACCTTGCTGCGCCGGTACAAC
ATCCCGCACGGGCCTGTAGTAGGATCAACTCGTAGGCTTTACGAGAAGAAGATCTTCGAG
TACGAGACCCAGAGGCGGCGGCTCTCGCCCCCCAGCTCGTCCGCCGCCTCCTCTTATAGC
TTCTCTGACTTGAATTCGACTAGAGGGGATGCAGATATGTATGATCTTCCCAAGAAAGAG
GACGCTTTACTCTACCAGAGCAAGGGCTACAATGACGACTACTATGAAGAGAGCTACTTC
ACCACCAGGACTTATGGGGAGCCCGAGTCTGCCGGCCCGTCCAGGGCTGTCCGCCAGTCA
GTGACTTCATTCCCAGATGCTGACGCTTTCCATCACCAGGTGCATGATGACGATCTTTTG
TCTTCTTCTGAAGAGGAGTGCAAGGATAGGGAACGCCCCATGTACGGCCGGGACAGTGCC
TACCAGAGCATCACGCACTACCGCCCTGTTTCAGCCTCCAGGAGCTCCCTGGACCTGTCC
TATTATCCTACTTCCTCCTCCACCTCTTTTATGTCCTCCTCATCATCTTCCTCTTCATGG
CTCACCCGCCGTGCCATCCGGCCTGAAAACCGTGCTCCTGGGGCTGGGCTGGGCCAGGAT
CGCCAGGTCCCGCTCTGGGGCCAGCTGCTGCTTTTCCTGGTCTTTGTGATCGTCCTCTTC
TTCATTTACCACTTCATGCAGGCTGAAGAAGGCAACCCCTTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 896
Start on reference sequence 132
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EMD
Symbol Nomenclature : EMD
SYNONYM : EDMD; LEMD5; STA
Designation : LEM domain containing 5
Full Nomenclature : emerin
GENEID : 2010
Locus Tag : XX-FW88778H2.1
GI : 61372644
GenBank Accession : AY891955
HGNC : 3331
MIM : 300384
Vega : OTTHUMG00000033186
Target GenBank: BC000738

 

Reference Sequence Alignment

  

HsCD00003111      ATGGACAACTACGCAGATCTTTCGGATACCGAGCTGACCACCTTGCTGCGCCGGTACAAC
NM_000117.2       ATGGACAACTACGCAGATCTTTCGGATACCGAGCTGACCACCTTGCTGCGCCGGTACAAC
                  ************************************************************

HsCD00003111      ATCCCGCACGGGCCTGTAGTAGGATCAACTCGTAGGCTTTACGAGAAGAAGATCTTCGAG
NM_000117.2       ATCCCGCACGGGCCTGTAGTAGGATCAACTCGTAGGCTTTACGAGAAGAAGATCTTCGAG
                  ************************************************************

HsCD00003111      TACGAGACCCAGAGGCGGCGGCTCTCGCCCCCCAGCTCGTCCGCCGCCTCCTCTTATAGC
NM_000117.2       TACGAGACCCAGAGGCGGCGGCTCTCGCCCCCCAGCTCGTCCGCCGCCTCCTCTTATAGC
                  ************************************************************

HsCD00003111      TTCTCTGACTTGAATTCGACTAGAGGGGATGCAGATATGTATGATCTTCCCAAGAAAGAG
NM_000117.2       TTCTCTGACTTGAATTCGACTAGAGGGGATGCAGATATGTATGATCTTCCCAAGAAAGAG
                  ************************************************************

HsCD00003111      GACGCTTTACTCTACCAGAGCAAGGGCTACAATGACGACTACTATGAAGAGAGCTACTTC
NM_000117.2       GACGCTTTACTCTACCAGAGCAAGGGCTACAATGACGACTACTATGAAGAGAGCTACTTC
                  ************************************************************

HsCD00003111      ACCACCAGGACTTATGGGGAGCCCGAGTCTGCCGGCCCGTCCAGGGCTGTCCGCCAGTCA
NM_000117.2       ACCACCAGGACTTATGGGGAGCCCGAGTCTGCCGGCCCGTCCAGGGCTGTCCGCCAGTCA
                  ************************************************************

HsCD00003111      GTGACTTCATTCCCAGATGCTGACGCTTTCCATCACCAGGTGCATGATGACGATCTTTTG
NM_000117.2       GTGACTTCATTCCCAGATGCTGACGCTTTCCATCACCAGGTGCATGATGACGATCTTTTG
                  ************************************************************

HsCD00003111      TCTTCTTCTGAAGAGGAGTGCAAGGATAGGGAACGCCCCATGTACGGCCGGGACAGTGCC
NM_000117.2       TCTTCTTCTGAAGAGGAGTGCAAGGATAGGGAACGCCCCATGTACGGCCGGGACAGTGCC
                  ************************************************************

HsCD00003111      TACCAGAGCATCACGCACTACCGCCCTGTTTCAGCCTCCAGGAGCTCCCTGGACCTGTCC
NM_000117.2       TACCAGAGCATCACGCACTACCGCCCTGTTTCAGCCTCCAGGAGCTCCCTGGACCTGTCC
                  ************************************************************

HsCD00003111      TATTATCCTACTTCCTCCTCCACCTCTTTTATGTCCTCCTCATCATCTTCCTCTTCATGG
NM_000117.2       TATTATCCTACTTCCTCCTCCACCTCTTTTATGTCCTCCTCATCATCTTCCTCTTCATGG
                  ************************************************************

HsCD00003111      CTCACCCGCCGTGCCATCCGGCCTGAAAACCGTGCTCCTGGGGCTGGGCTGGGCCAGGAT
NM_000117.2       CTCACCCGCCGTGCCATCCGGCCTGAAAACCGTGCTCCTGGGGCTGGGCTGGGCCAGGAT
                  ************************************************************

HsCD00003111      CGCCAGGTCCCGCTCTGGGGCCAGCTGCTGCTTTTCCTGGTCTTTGTGATCGTCCTCTTC
NM_000117.2       CGCCAGGTCCCGCTCTGGGGCCAGCTGCTGCTTTTCCTGGTCTTTGTGATCGTCCTCTTC
                  ************************************************************

HsCD00003111      TTCATTTACCACTTCATGCAGGCTGAAGAAGGCAACCCCTTCTTG
NM_000117.2       TTCATTTACCACTTCATGCAGGCTGAAGAAGGCAACCCCTTCTAG
                  *******************************************:*

Pathways

Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Clearance of Nuclear Envelope Membranes from Chromatin
Reactome : Depolymerisation of the Nuclear Lamina
Reactome : Initiation of Nuclear Envelope Reformation
Reactome : M Phase
Reactome : Mitotic Anaphase
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Mitotic Prophase
Reactome : Nuclear Envelope Breakdown
Reactome : Nuclear Envelope Reassembly

Protein

SMART domain : LEM : in nuclear membrane-associated proteins
UniProt : Q6FI02
UniProt : P50402
HPRD : 02309

Clone

Cloning Information : 24062
HIP Master Clone ID : 54438
Original Clone ID : FLH024062.01L

Organism


TAX_ID : 9606
Species Specific ID: 2010

Genome

Chromosome : X
Map Location : Xq28
Ensembl : ENSG00000102119

Reagents

Labome : emerin-antibody