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AT1G04970 (Arabidopsis thaliana Columbia) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  AT1G04970
Gene Name:  lipid-binding serum glycoprotein family protein
Sequence              Map: pDONR cofig.pdf
Original Clone ID: 6002.1.44_GO.600
Keyword: full-length cds (alternative splice form)
Species: Arabidopsis thaliana Columbia
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: Parent TargetDB: GO.599; This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00313482

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1068nts         Open reading frame : 51 to 1118

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGAAATCGGGCTCTCTCTAGGCTTGAAAAGTGATGAAGGAGGCTTAAAAC
TTTCTCTTTCGGAATGTGGATGCCATGTGGAAGACATTACCATTGAGTTGGAAGGAGGAG
CATCATGGTTTTATCAGGGGATGGTTAATGCATTTAAAGACCAAATTGGGTCAAGTGTGG
AAAGTACCATTGCCAAGAAACTCACGGAAGGAGTATCAGACCTTGATTCATTCCTACAAA
GCCTTCCAAAGGAAATCCCAGTGGATGATAATGCTGACCTTAATGTCACTTTCACCAGTG
ATCCTATATTGAGAAATTCATCTATCACTTTTGAGATTGATGGCTTGTTCACCAAAGGAG
AAACAAATCAAGTCTTAAAATCCTTCTTCAAAAAGTCTGTATCTTTAGTTATCTGCCCTG
GAAATTCTAAAATGCTCGGAATTTCAGTTGACGAAGCTGTTTTTAACTCTGCTGCAGCTT
TGTACTATAATGCAGACTTTGTGCAATGGGTTGTGGATAAAATACCAGAGCAGTCTCTTT
TAAACACTGCTAGGTGGAGGTTCATCATTCCACAACTATACAAGAAATACCCGAACCAGG
ATATGAATCTGAACATCAGTTTGTCCTCACCTCCGCTTGTAAAGATATCAGAGCAATACG
TTGGAGCTAATGTTAATGCAGACCTAGTAATCAATGTTCTAGATGCAAACCAAGTAATAC
CTGTAGCTTGCATCTCCTTGATGATCCGGGGATCTGGTGCTCTCAGAGTCATGGGCAATA
ACCTTGGAGGCAGCGTAAGTTTAGAAGATTTCTCCATGTCTTTGAAATGGAGCAACATTG
GAAATCTCCATCTGCATCTTCTTCAGCCAATAGTGTGGACTGTTATACAAACAGTGTTTG
TGCCATATGCAAATGACCACCTAGAAAAGGGATTCCCGTTGCCCATAATGCACGGATTCA
CACTTCAGAATGCGGAAATCATCTGCTCAGAATCTGAAATCACAGTTTGCAGCGATGTCG
CCTACTTGGATTCGTCCCAACAGCCTCAATGGCTTTGATAGGACCCAGCTTTCTTGTACA
AAGTTGGCATTATAAGAAAGCATTGCTTA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
CCAACTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.600
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AT1G04970
SYNONYM : F13M7.4; F13M7_4
GENEID : 839357
Locus Tag : AT1G04970
GI : 30679067
GenBank Accession : NM_179262
TAIR : AT1G04970
Target GenBank: NM_179262

 

Protein

SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
Structural Annotation Wiki : TOPSAN
UniProt : Q9MAU5

Clone

Cloning Information : 513741
HIP Master Clone ID : 424908
Original Clone ID : 6002.1.44_GO.600

Experimental

TargetTrack - Experimental data : GO.600

Organism


TAX_ID : 3702
Species Specific ID: 839357

Genome

Chromosome : 1

Reagents

Labome : AT1G04970-cDNA