DNASU Plasmid Repository • 480.965.5697 | Email

LRRC46 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  LRRC46
Gene Name:  leucine rich repeat containing 46
Sequence              Map: pDONR cofig.pdf
Original Clone ID: 5545.1.92_GO.40160
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00313712

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 984nts         Open reading frame : 51 to 1034

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCTCTGGAGGGAAGTCAGCCCAGGGTCCAGAGGAAGGGGGCGTCTGCATCA
CTGAAGCCCTTATCACTAAGCGGAACTTGACTTTCCCTGAAGATGGGGAACTGTCAGAGA
AGATGTTTCACACTCTTGATGAACTGCAGACTGTCCGCCTGGACCGGGAGGGGATTACTA
CTATCAGGAACTTAGAAGGCCTCCAGAATCTTCACAGTCTCTATCTGCAAGGGAATAAGA
TCCAGCAAATTGAGAACCTGGCTTGCATCCCCTCCTTGCGCTTCCTGTCTCTGGCAGGAA
ACCAAATCAGGCAGGTGGAAAACCTCCTCGACCTCCCATGCCTCCAGTTTCTGGACCTTT
CTGAGAACCTGATAGAAACATTGAAGCTGGATGAGTTCCCCCAGAGCCTTCTCATCCTCA
ACCTGTCTGGAAACAGCTGCACCAACCAGGATGGCTACCGCGAGCTGGTGACAGAAGCCC
TGCCACTTCTCCTGGACCTGGACGGGCAGCCTGTGGTGGAGCGCTGGATTTCGGATGAGG
AGGATGAAGCCTCAAGCGATGAGGAGTTCCCAGAGCTGAGTGGCCCATTCTGCTCAGAAC
GAGGCTTCCTCAAGGAGCTGGAGCAGGAGCTGAGCAGGCACAGGGAGCACCGGCAACAGA
CGGCCCTGACAGAGCACCTGCTGAGGATGGAGATGCAGCCCACCCTCACCGACCTGCCCC
TGCTACCTGGGGTGCCCATGGCTGGGGACAGCAGCCCTTCTGCCACTCCTGCGCAAGGGG
AGGAGACAGTCCCTGAGGCCGTCTCCTCACCCCAGGCCTCCTCTCCCACCAAGAAACCAT
GCAGTCTGATTCCCAGGGGCCACCAAAGCTCTTTCTGGGGAAGGAAGGGGGCACGAGCAG
CCACAGCCCCCAAGGCCTCTGTGGCTGAGGCCCCCAGCACAACCAAAACTACGGCCAAGA
GAAGCAAGAAATGATAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATT
GCTTA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
CCAACTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.40160
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site Att P2 Primer 3026 3043
primer site U19 primer 517 535
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
selectable marker KanR kanamycin resistance gene 3156 3965
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRC46
Symbol Nomenclature : LRRC46
Designation : leucine-rich repeat-containing protein 46
Full Nomenclature : leucine rich repeat containing 46
GENEID : 90506
GI : 15529980
GenBank Accession : NM_033413
HGNC : 25047
Vega : OTTHUMG00000178289
Target GenBank: NM_033413

 

Protein

SMART domain : LRR_SD22 : Leucine-rich repeat, SDS22-like subfamily
Structural Annotation Wiki : TOPSAN
UniProt : Q96FV0
UniProt : A8K9Q0
HPRD : 08320

Clone

Cloning Information : 514414
HIP Master Clone ID : 425581
Original Clone ID : 5545.1.92_GO.40160

Experimental

TargetTrack - Experimental data : GO.40160

Organism


TAX_ID : 9606
Species Specific ID: 90506

Genome

Chromosome : 17
Map Location : 17q21.32
Ensembl : ENSG00000141294

Reagents

Labome : LRRC46-antibody