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SYTL4 (Homo sapiens) in pSGX3 (His-tagged bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  SYTL4
Gene Name:  synaptotagmin-like 4
Sequence             
Original Clone ID: 13071a4BCt3p1
Keyword: partial length cds 352-488 of 671 aa
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: "C-terminal 6-His, TOPO"
Mutation/
Discrepancy :
No / No
Publications: PMID: 24184645
Title: The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids.
Authors: PSI
New York SGX Research Center for Structural Genomics
HsCD00321956

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Special MTA: PSI

 

Insert sequence: 438nts         Open reading frame : 51 to 488

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTGGGAACATCT
TTGTGACTGGCAGGATTGCCTTTTCCCTGAAGTATGAGCAGCAAACCCAGAGTCTGGTTG
TCCATGTGAAGGAGTGCCATCAGCTGGCCTATGCTGATGAAGCCAAGAAGCGCTCTAACC
CATATGTGAAGACTTACCTTCTGCCTGACAAGTCCCGCCAAGGAAAAAGAAAAACCAGCA
TCAAGCGGGACACTGTTAATCCACTATATGATGAGACGCTGAGGTATGAGATCCCAGAAT
CTCTCCTGGCCCAGAGGACCCTGCAGTTCTCAGTTTGGCATCATGGTCGTTTTGGCAGAA
ACACTTTCCTTGGAGAGGCAGAGATCCAGATGGATTCCTGGAAGCTTGATAAGAAACTGG
ATCATTGCCTCCCTTTACATGGAAAGATCAGTGCTGAGTCCGAAGGGCATCATCACCATC
ACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-13071a
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SYTL4
Symbol Nomenclature : SYTL4
SYNONYM : SLP4
Designation : exophilin-2|granuphilin-a|synaptotagmin-like protein 4
Full Nomenclature : synaptotagmin-like 4
GENEID : 94121
Locus Tag : RP11-524D16__A.2
GI : 193804860
GenBank Accession : NM_080737
HGNC : 15588
MIM : 300723
Vega : OTTHUMG00000022004
Target GenBank: None

 

Protein

PDB - Structural Annotation : 3FDW
SMART domain : C2 : Protein kinase C conserved region 2 (CalB)
Structural Annotation Wiki : TOPSAN
UniProt : B2R7R4
UniProt : Q96C24
UniProt : A8K973
UniProt : Q71SF7
HPRD : 06735
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 516251
HIP Master Clone ID : 426571
Original Clone ID : 13071a4BCt3p1

Experimental

Structural Biology Knowledgebase : SBKB (3FDW)
TargetTrack - Experimental data : NYSGXRC-13071a

Organism


TAX_ID : 9606
Species Specific ID: 94121

Genome

Chromosome : X
Map Location : Xq21.33
Ensembl : ENSG00000102362

Reagents

Labome : SYTL4-antibody