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VAMP8 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  VAMP8
Gene Name:  vesicle-associated membrane protein 8
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110800.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003233

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 303nts         Open reading frame : 1 to 303
Reference Sequence Alignment

ATGGAGGAAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTG
GAGGGAGTTAAGAATATTATGACCCAGAATGTGGAGCGGATCCTGGCCCGGGGGGAAAAC
TTGGAACATCTCCGCAACAAGACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACG
ACATCGCAGAAGGTGGCTCGGAAATTCTGGTGGAAGAACGTGAAGATGATTGTCCTTATC
TGCGTGATTGTTTTTATCATCATCCTCTTCATTGTGCTCTTTGCCACTGGTGCCTTCTCT
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 356
Start on reference sequence 54
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : VAMP8
Symbol Nomenclature : VAMP8
SYNONYM : EDB; VAMP-8
Designation : vesicle-associated membrane protein 8 (endobrevin)
Full Nomenclature : vesicle-associated membrane protein 8
GENEID : 8673
GI : 60655562
GenBank Accession : AY889554
HGNC : 12647
MIM : 603177
Vega : OTTHUMG00000130180
Target GenBank: BC001634

 

Reference Sequence Alignment

  

HsCD00003233      ATGGAGGAAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTG
NM_003761.4       ATGGAGGAAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTG
                  ************************************************************

HsCD00003233      GAGGGAGTTAAGAATATTATGACCCAGAATGTGGAGCGGATCCTGGCCCGGGGGGAAAAC
NM_003761.4       GAGGGAGTTAAGAATATTATGACCCAGAATGTGGAGCGGATCCTGGCCCGGGGGGAAAAC
                  ************************************************************

HsCD00003233      TTGGAACATCTCCGCAACAAGACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACG
NM_003761.4       TTGGAACATCTCCGCAACAAGACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACG
                  ************************************************************

HsCD00003233      ACATCGCAGAAGGTGGCTCGGAAATTCTGGTGGAAGAACGTGAAGATGATTGTCCTTATC
NM_003761.4       ACATCGCAGAAGGTGGCTCGAAAATTCTGGTGGAAGAACGTGAAGATGATTGTCCTTATC
                  ********************.***************************************

HsCD00003233      TGCGTGATTGTTTTTATCATCATCCTCTTCATTGTGCTCTTTGCCACTGGTGCCTTCTCT
NM_003761.4       TGCGTGATTGTTTTTATCATCATCCTCTTCATTGTGCTCTTTGCCACTGGTGCCTTCTCT
                  ************************************************************

HsCD00003233      TAG
NM_003761.4       TAA
                  **.

Pathways

Panther : 5HT2 type receptor mediated signaling pathway
Panther : 5HT3 type receptor mediated signaling pathway
Panther : 5HT4 type receptor mediated signaling pathway
Panther : Beta1 adrenergic receptor signaling pathway
Panther : Beta2 adrenergic receptor signaling pathway
Panther : Beta3 adrenergic receptor signaling pathway
Panther : Cortocotropin releasing factor receptor signaling pathway
Panther : Dopamine receptor mediated signaling pathway
Panther : Ionotropic glutamate receptor pathway
Panther : Metabotropic glutamate receptor group II pathway
Panther : Metabotropic glutamate receptor group III pathway
Panther : Muscarinic acetylcholine receptor 1 and 3 signaling pathway
Panther : Muscarinic acetylcholine receptor 2 and 4 signaling pathway
Panther : Nicotinic acetylcholine receptor signaling pathway
Panther : Opioid prodynorphin pathway
Panther : Opioid proenkephalin pathway
Panther : Opioid proopiomelanocortin pathway
Panther : Oxytocin receptor mediated signaling pathway
Panther : Thyrotropin-releasing hormone receptor signaling pathway
Reactome : Clathrin derived vesicle budding
Reactome : Golgi Associated Vesicle Biogenesis
Reactome : Lysosome Vesicle Biogenesis
Reactome : Membrane Trafficking
Reactome : trans-Golgi Network Vesicle Budding

Protein

UniProt : Q9BV40
HPRD : 04415

Clone

Cloning Information : 110800
HIP Master Clone ID : 87083
Original Clone ID : FLH110800.01X

Organism


TAX_ID : 9606
Species Specific ID: 8673

Genome

Chromosome : 2
Map Location : 2p12-p11.2
Ensembl : ENSG00000118640

Reagents

Labome : VAMP8-antibody