| Gene: |
Gene Symbol:
None Gene Name: None Sequence |
|---|---|
| Original Clone ID: | 72284 |
| Keyword: | SP or TM removed |
| Species: | Bacteroides uniformis ATCC 8492 |
| Type: | cDNA |
| Vector Name: | pSpeedET Format: FUSION |
| Source: | Joint Center for Structural Genomics |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / No |
| Publications: | None |
| Authors: |
PSI Joint Center for Structural Genomics |
|
BuCD00323338 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI |
Insert sequence: 330nts
Open reading frame : 51 to 380CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGATGAGGATG
ATAAGGTCGAAATTCCTCAACTGGTAGGTAAGTGGATTGTGAAAGAACCAGTCCTGCAGG
ACGATTTTGTGACCTGCTATACGTTTAATGCAGACAAGACCTATGAAGTTTATACCGGAA
GCCCGTTATCTAACGGAGTCCCTTTTCGTGGAACTTATATCATAAGTCTTGATGAAAAAT
TGATTAAGTTGTATGATAAAGAGGAACATTGTACTGAACAATATCATATTTTGAAACTGA
CGTCCAAGGAGATGAAGTGGGAGAACGCATCGCCCAAAGACGGGAATTCTGACAAACGGC
TTGAGAAGTACAACGATTAACGCGACTTAATTAA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Mutations: None |
Discrepancy: None |
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC |
|
3' Linker Sequence:
TAACGCGACTTAATTAA |
|
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
kanamycin Bacterial Selection Condition: 50ug/mL kanamycin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors. |
| Protein Expression Results: |
ProteinExpressed: Not_Applicable ProteinPurified: Not_Applicable SolubleProtein: Not_Applicable Find experimental protocols in 417984 |
| Recommended expression in: | HK100 |
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||
| Vector Name: | pSpeedET |
pSpeedET in advanced viewed |
| Synonyms: | pSpeed-ET | |
| Sequencing Primer: | Forward:
pBAD forward
Reverse: alt_bad_rev |
|
| Description: | Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning. | |
| Comments: | If this vector contains an insert, the ccdB death cassette will not be present. | |
| Size (bp): | 5464 | |
| Parent Vector: | None | |
| Empty Vector: | EvNO00285791 | |
| Properties: | PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker | |
| Author Name: |
Joint Center for Structural Genomics |
|
| Publications: |
PMID:
18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | ori | ColE1-type origin of replication | 3306 | 3988 |
| inducible trxn activator protein gene | araC | araC coding sequence | 4563 | 5438 |
| negative selection marker | ccdB | ccdB death cassette gene (lost in with-insert form) | 1596 | 1805 |
| promoter | araC | araC promoter | 256 | 285 |
| promoter | T7 | T7 promoter | 290 | 310 |
| protease cleavage site | TEV | TEV protease cleavage site | 382 | 402 |
| selectable marker | CmR | chloramphenicol resistance gene (CmR) (lost in with-insert form) | 502 | 1158 |
| selectable marker | KanR | kanamycin resistance gene | 2396 | 3208 |
| tag | His | N-terminal 6xHis tag | 364 | 381 |
| trxn regulatory element | arabinose O2 operator | arabinose O2 operator | 5 | 23 |
| trxn regulatory element | arabinose O1 operator | arabinose O1 operator | 161 | 182 |
| trxn termination sequence | rrnB | rrnB T2 transcription terminator | 2258 | 2284 |
