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PTPN7 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PTPN7
Gene Name:  protein tyrosine phosphatase, non-receptor type 7
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110199.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003247

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1083nts         Open reading frame : 1 to 1083
Reference Sequence Alignment

ATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACCTTGTCTTTGGGGGCA
GCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCATGTGCGACTGCAGGAG
AGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTGGGGGCCGTAGAACCC
ATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTGCGCACTGCTGGACAC
CCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAGCAACTGGAAGAAGAA
TTCTTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGACATCCCTGGCCACGCC
TCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAGAGCCGTGTCTGTCTAGGCCGG
GCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATCCGAGGCTATGACGGG
AAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACTGTGTCGGACTTCTGG
GAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACTCAGCTCCGAGAGGGC
AAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTATGGACCCTTCCAGATC
CGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAGCTCACCATCCAGTAC
CAGGAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGGCCAGACCATCAGACA
CCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAGGAGAGCCCGGAGACA
GCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGGATTGGCCGGACGGGCTGC
TTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGAGAAGTGGACATTCTG
GGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAGACGGCAGAGCAGTAC
CAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCTGAGGAACCCAGCCCC
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1672
Start on reference sequence 590
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PTPN7
Symbol Nomenclature : PTPN7
SYNONYM : BPTP-4; HEPTP; LC-PTP; LPTP; PTPNI
Designation : dual specificity phosphatase 1|hematopoietic protein-tyrosine phosphatase|protein-tyrosine phosphatase LC-PTP|protein-tyrosine phosphatase, nonreceptor-type, stress induced|tyrosine-protein phosphatase non-receptor type 7
Full Nomenclature : protein tyrosine phosphatase, non-receptor type 7
GENEID : 5778
Locus Tag : RP11-294K24.3
GI : 60655580
GenBank Accession : AY889563
HGNC : 9659
MIM : 176889
Vega : OTTHUMG00000035931
Target GenBank: BC001746

 

Reference Sequence Alignment

  

HsCD00003247        ------------------------------------------------------------
NM_002832.3         ATGGTCGGGAAGGCCTGGCCCCTCACCCATTCCCAGGGAACGGGGCCTTGGGCCCCAGAA
NM_001199797.1      ------------------------------------------------------------
NM_080588.2         ------------------------------------------------------------
                                                                                

HsCD00003247        ------------------------------------------------------------
NM_002832.3         GGGCACAGGCGGGAGGCAGCCGACCCCTGGTGGCAGCGGCAGCAGGCACAGGAGGGCAGA
NM_001199797.1      ---------------------------------ATGAACCGGCTGGGCTGCTGGCCCGCA
NM_080588.2         ------------------------------------------------------------
                                                                                

HsCD00003247        ------------------------------------------------------------
NM_002832.3         ATGCAGCTGGGGTGTGCGTGGGTGGCAGCAAGGAGGGGCGGAGGGAGGAAGCTGGCTTCC
NM_001199797.1      AGGAGCCTGCCAAGGTCAGGCAGGGGCAGTGGGCAGAGGAGTCTTGGACTTGGGGGCCAC
NM_080588.2         ------------------------------------------------------------
                                                                                

HsCD00003247        ------------------------------------------------------------
NM_002832.3         TGGAGCCTTCTCAGCCCTCAAAGACAGACCGACAGACAGACAGACAGCTGGCAAGAGGCA
NM_001199797.1      CCTTCAGGTCCCAGGCATCCAGGCACCCCAGCAGCGAAGAGGACATGGGAGTCCCCTGGT
NM_080588.2         ------------------ATGGGAGCCTCCTTCTGGCCAATCAGGCAGGCCAGAGAACAG
                                                                                

HsCD00003247        ------------------------------------------------------------
NM_002832.3         GCCTGGGGGCCACAGCTGCTTCAGCAGACCTCATGGCTGAGTGAGCCTCCCCTGGGCCCA
NM_001199797.1      GTGAGGGCCAGAGACTGTCCACTGTCTACCTCATGGCTGAGTGAGCCTCCCCTGGGCCCA
NM_080588.2         CAGCGGAGGGCCCTGTCTTTCAGGCAGACCTCATGGCTGAGTGAGCCTCCCCTGGGCCCA
                                                                                

HsCD00003247        ---------------ATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACC
NM_002832.3         GCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACC
NM_001199797.1      GCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACC
NM_080588.2         GCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACC
                                   *********************************************

HsCD00003247        TTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCAT
NM_002832.3         TTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCAT
NM_001199797.1      TTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCAT
NM_080588.2         TTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCAT
                    ************************************************************

HsCD00003247        GTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTG
NM_002832.3         GTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTG
NM_001199797.1      GTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTG
NM_080588.2         GTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTG
                    ************************************************************

HsCD00003247        GGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTG
NM_002832.3         GGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTG
NM_001199797.1      GGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTG
NM_080588.2         GGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTG
                    ************************************************************

HsCD00003247        CGCACTGCTGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAG
NM_002832.3         CGCACTGCTGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAG
NM_001199797.1      CGCACTGCTGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAG
NM_080588.2         CGCACTGCTGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAG
                    ************************************************************

HsCD00003247        CAACTGGAAGAAGAATTCTTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGAC
NM_002832.3         CAACTGGAAGAAGAATTCTTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGAC
NM_001199797.1      CAACTGGAAGAAGAATTCTTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGAC
NM_080588.2         CAACTGGAAGAAGAATTCTTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGAC
                    ************************************************************

HsCD00003247        ATCCCTGGCCACGCCTCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAGAGCCGT
NM_002832.3         ATCCCTGGCCACGCCTCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAGAGCCGT
NM_001199797.1      ATCCCTGGCCACGCCTCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAGAGCCGT
NM_080588.2         ATCCCTGGCCACGCCTCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAGAGCCGT
                    ************************************************************

HsCD00003247        GTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATC
NM_002832.3         GTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATC
NM_001199797.1      GTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATC
NM_080588.2         GTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATC
                    ************************************************************

HsCD00003247        CGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACT
NM_002832.3         CGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACT
NM_001199797.1      CGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACT
NM_080588.2         CGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACT
                    ************************************************************

HsCD00003247        GTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACT
NM_002832.3         GTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACT
NM_001199797.1      GTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACT
NM_080588.2         GTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACT
                    ************************************************************

HsCD00003247        CAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTAT
NM_002832.3         CAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTAT
NM_001199797.1      CAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTAT
NM_080588.2         CAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTAT
                    ************************************************************

HsCD00003247        GGACCCTTCCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAG
NM_002832.3         GGACCCTTCCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAG
NM_001199797.1      GGACCCTTCCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAG
NM_080588.2         GGACCCTTCCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAG
                    ************************************************************

HsCD00003247        CTCACCATCCAGTACCAGGAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGG
NM_002832.3         CTCACCATCCAGTACCAGGAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGG
NM_001199797.1      CTCACCATCCAGTACCAGGAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGG
NM_080588.2         CTCACCATCCAGTACCAGGAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGG
                    ************************************************************

HsCD00003247        CCAGACCATCAGACACCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAG
NM_002832.3         CCAGACCATCAGACACCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAG
NM_001199797.1      CCAGACCATCAGACACCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAG
NM_080588.2         CCAGACCATCAGACACCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAG
                    ************************************************************

HsCD00003247        GAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGGATT
NM_002832.3         GAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGGATT
NM_001199797.1      GAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGGATT
NM_080588.2         GAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGGATT
                    ************************************************************

HsCD00003247        GGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGA
NM_002832.3         GGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGA
NM_001199797.1      GGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGA
NM_080588.2         GGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGA
                    ************************************************************

HsCD00003247        GAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAG
NM_002832.3         GAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAG
NM_001199797.1      GAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAG
NM_080588.2         GAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAG
                    ************************************************************

HsCD00003247        ACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCT
NM_002832.3         ACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCT
NM_001199797.1      ACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCT
NM_080588.2         ACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCT
                    ************************************************************

HsCD00003247        GAGGAACCCAGCCCCTAG
NM_002832.3         GAGGAACCCAGCCCCTGA
NM_001199797.1      GAGGAACCCAGCCCCTGA
NM_080588.2         GAGGAACCCAGCCCCTGA
                    ****************..

Pathways

NCI : Downstream signaling in naïve CD8+ T cells
NCI : Ras signaling in the CD4+ TCR pathway
WikiPathway : MAPK signaling pathway

Clone

Cloning Information : 110199
HIP Master Clone ID : 86482
Original Clone ID : FLH110199.01X

Organism


TAX_ID : 9606
Species Specific ID: 5778

Genome

Chromosome : 1
Map Location : 1q32.1
Ensembl : ENSG00000143851

Reagents

Labome : PTPN7-antibody