BF0058 (B. fragilis NCTC 9343 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BF0058
Gene Name:  hypothetical protein
Original Clone ID:
69061
Keyword:
SP or TM removed
Species:
Bacteroides fragilis NCTC 9343
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
BfCD00327446
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1086nts
Open reading frame: 51 to 1136

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCAAAGAATTGC
ATGAAAGCAGTACAATTATTTCTTTTAAAGAACATACTGATGTAAATGCGGATAGTATAT
TAGAGTTATCTTTCTTAAAGCTTCAAACAAAAGATTCATGTTTGGTAAAGAATGTTGGGC
TTATTAGAGAATTAAATAATTGTTTATTAATATTGGATTCTGCCAACTCTAATTTGTATG
TTTTTAACAAATCAGGAGCTTTTGTAAATCAGATAGGACAGAAGGGAAGTGGCCCCGGTG
AATATATATTGTTATCTTCATTTTTTGTAGACAATAATAAAAATTATATTGCCGCAATTG
ATATTGCCCAAGATAAAGTATTGTATTACAATGCGACTGATTTTAGTTTTTTGTACGAAA
GGAGATTACCTTTTAGTACATCTTGCTGTTTACAGTTAGAGGATGGTAATTTGCTTTGGA
ATAGTAGAGAATATACAGACTCTAAATTATCTGACTTCTATTTTGTGGTTACAGATTCTT
TGTTTGATATTATAGATTATAAAATGAATAAAGAGTTTAAATCTGGTTATACAACAGGGC
CTTCTCAAATGATATATAAGGTTGGAACCAATGTATTTGCATATACTCCTTTTGATTTGA
CGATTTATAGAGTGGGTACTTCTGAGATAGTTCCTGCTCATTCTTTTTCATTTGAGGGTA
CTGATATCCCTTCTTTAGATTTTCTGAATAAAACTAGTAATCAGGGAAACTCTAATTACT
TATATGATCTGATTCAATCCGATTATATTTCTTATTATTGTGTAGAAGAAACTGAACGTG
ATTTGTTTGTATGCTATATGAAAAATAAAGAAAAGTATATAGGGTTGTATGATAAGAATA
CTGATAGAACGTATAATTATCCAATTAAAATATTTCAGGATCAGTTAAAGGTCGGTGAAC
TGAATTATTTTAGTATAGGTTCAGTTGATGATTATCATGTGGCTCCATTGGATGTATTAT
CCTTAAAAGATATGGCAGGAAATGGATATGTGTTTGATGATAAGTTGTCTGAATTGCTAA
CAATATCGAACGAAGAGGATAATTCTATATTATTATTTGTTCGGATTAAAAAATAACGCG
ACTTAATTAA

Protein sequence predicted based on nucleotide sequence

KELHESSTIISFKEHTDVNADSILELSFLKLQTKDSCLVKNVGLIRELNNCLLILDSANS
NLYVFNKSGAFVNQIGQKGSGPGEYILLSSFFVDNNKNYIAAIDIAQDKVLYYNATDFSF
LYERRLPFSTSCCLQLEDGNLLWNSREYTDSKLSDFYFVVTDSLFDIIDYKMNKEFKSGY
TTGPSQMIYKVGTNVFAYTPFDLTIYRVGTSEIVPAHSFSFEGTDIPSLDFLNKTSNQGN
SNYLYDLIQSDYISYYCVEETERDLFVCYMKNKEKYIGLYDKNTDRTYNYPIKIFQDQLK
VGELNYFSIGSVDDYHVAPLDVLSLKDMAGNGYVFDDKLSELLTISNEEDNSILLFVRIK
K*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC
3' Linker Sequence:
TAACGCGACTTAATTAA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in 386541
Recommended expression in:
Not Applicable