Shew_1452 (S. loihica PV-4 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Shew_1452
Gene Name:  AraC family transcriptional regulator
Original Clone ID:
49168
Keyword:
None
Species:
Shewanella loihica PV-4
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SsCD00327539
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1512nts
Open reading frame: 51 to 1562

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCAGGCTG
AGGCGCAGCATCCCAAGGCGGAGCAGTATCGCACCGCGCGTCTGTCACGGGACGCCAGAT
TCGATGGCCTGTTCTTCGTCGGCGTCTTCTCCACCGGCATCTTTTGTCGTCCTATCTGCC
CGGCGGTGCCTGCCAAGGAGGCCAATGTGAGGTATTTCGACGATGCCCTGTCGGCGGCTC
AGGCTGGCCTGAGGCCCTGTCTGCGTTGTCGCCCGGACAGCGCGCCGGGCTCAAATCCCT
GGCTTGGTAAACAGACCAGCCTGAATCGCGCCATGAGTCTCATCGACGCCGGCGTCTTGT
GCGGCCCCCAGGCGATCCCTATGACTGGGCTCGCCGAGCGATTAGGGATCAGCGAGCGTT
ACCTGAGGCAACTGTTTCACGACAGCCTGGGCACCTCGCCCAAGCAATATGCCCTGTATC
AACAGCTGATGTTTGCCAAACAGTTGCTGCATCAGACCAAGATGCCGATCACTCAGGTGG
CCTATGCCGCCGGGTTTAACAGCGTGAGGCGCTTCAACGAGATCTTTCGCCAGCAGCTGC
AGTTGACCCCGAGTGAGATCCGTAAGGCCACGGGCCAGCCGTCGCTGCCCGTCCAGGGCC
TGACACTCAAGCTTGCTTATCGTCCGCCCTATGATTGGCAACACCTGCGCAGCTTCTTCG
CACAGCGCGCCGTGCCCGAGATGGAGTGGCTAGATGAACAGAGCTATGGCCGCAGCTTCG
TGCTCGGCGCCGCCAAGGGCTATTTCTGCGCAGTCCATAGTGAGCAAGAGGCCTGCTTTC
ATGTCACCATTCATCTCGCCGAGGCGGACTCACTCAGCCAGCTCAAGGGGATCATCAGTC
ATATTCGTCGCATTCTGGATCTCGACGCCGACATGCTCACCATAGGCGATAAGCTGCAAG
CGCTGGGTAAGGCTCTGCCTCAGGTATCGCCTGGCCTGCGTCTGCCCGGCGTCTGGTCGC
CTTTCGAGGCGGCTTGCCGCGCCATCTTAGGTCAGCAGGTGAGTGTGACCCAGGCGATCA
AGCTCTTGGGGCAACTGGTGGCCGATCATGGCGAGACGATTAGCCTAAATGAAAAAACGG
TGCGTCTGTTCCCGACTCCTGCGGCGCTAGTGGATGCCAGCCTGGATGGGCTTAAGATGC
CGGGGATCAGAAAAGAGGCGATTCGTGCCCTGGCGGCCTTTGTGATGGCTAACCCCGAGT
CGGAAATAGACGACTGGCTACAGATCAAGGGGATAGGCCCCTGGACTTTGGATTATGTGC
GCCTGCGGGGCCTGAGCCTGCCAGACGTGCTGCTGGTTGGCGATCTCGTGGTCAAACAGC
AACTGCTGGTGAACCTGCGACCCGAGCCGTCCAGTTGTGATGGTGAGCCTGCTGAAGACG
AAGATCCCAAGGCGGCGAAGAAGCGGGCGCAGGCACAATATCAGGCGCTCAGCCAAGCCG
TGCTACGGCAGGTCGCCCCCTGGGGCAGTTATCTCACCCTGCAGCTCTGGAATCGGCAGT
AACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGA

Protein sequence predicted based on nucleotide sequence

MQAEAQHPKAEQYRTARLSRDARFDGLFFVGVFSTGIFCRPICPAVPAKEANVRYFDDAL
SAAQAGLRPCLRCRPDSAPGSNPWLGKQTSLNRAMSLIDAGVLCGPQAIPMTGLAERLGI
SERYLRQLFHDSLGTSPKQYALYQQLMFAKQLLHQTKMPITQVAYAAGFNSVRRFNEIFR
QQLQLTPSEIRKATGQPSLPVQGLTLKLAYRPPYDWQHLRSFFAQRAVPEMEWLDEQSYG
RSFVLGAAKGYFCAVHSEQEACFHVTIHLAEADSLSQLKGIISHIRRILDLDADMLTIGD
KLQALGKALPQVSPGLRLPGVWSPFEAACRAILGQQVSVTQAIKLLGQLVADHGETISLN
EKTVRLFPTPAALVDASLDGLKMPGIRKEAIRALAAFVMANPESEIDDWLQIKGIGPWTL
DYVRLRGLSLPDVLLVGDLVVKQQLLVNLRPEPSSCDGEPAEDEDPKAAKKRAQAQYQAL
SQAVLRQVAPWGSYLTLQLWNRQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC
3' Linker Sequence:
TAACGCGACTTAATTAA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 396809
Recommended expression in:
Not Applicable