SAV0400 (S. aureus subsp. aureus Mu50 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SAV0400
Gene Name:  NLP/P60 family lipoprotein
Original Clone ID:
70015
Keyword:
SP or TM removed
Species:
Staphylococcus aureus subsp. aureus Mu50
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 24051416
Title: Structures of a Bifunctional Cell Wall Hydrolase CwlT Containing a Novel Bacterial Lysozyme and an NlpC/P60 dl-Endopeptidase.
Authors:
PSI
Joint Center for Structural Genomics
SaCD00328273
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 939nts
Open reading frame: 51 to 989

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGATGATACGG
ACAGTGGCGAAAACAACAAGGATTCTTCTATTCCACAAGGCGGTGTGACCGTTTCACCAG
AAGTGTTGGCACACCGTCCGTTGATTGAAAAATATGGTAAGGAATATGGGATTGAAGACT
ATGTTTCTTATATCTTAGCCATTATGCAAGTGGAATCGGGCGGTACAGCAGAAGATGTTA
TGCAAAGTTCCGAATCTTTGGGTTTACCGCCCAACAGTTTGAGTACCGAAGAGTCCATCA
AACAAGGGGTTAAATATTTCAGTGAATTATTAACCAGTGCAGAACAACAAGGAGTGGATA
TAGATTCTGTTATCCAATCTTATAATTATGGCGGTGGCTTTTTAAATTATGTGAGGAGTC
ATGGGAAAAAATATACCTATGAATTAGCCGAACAGTTTTCTAAAGAGAAGTCGGGTGGTC
AAAAAGCAGACTATCCCAATCCTATAGCCATACCTGTGAATGGTGGTTGGCGATATAACT
ATGGAAATCAGTTTTATGTTCAGTTGGTTTCACAATACCTAACCGACACTTCCCCAACAG
AATTTGATGATGAAACCGTGCAAGTCATTATGGACGAAGCCTTGAAATACGAAGGGTTTC
CCTATGTCTTTGGAGGGGCGTCGCCTACCACCTCTTTTGATTGTAGTGGCTTAATACAAT
GGGTGTATGACAAAGCTGGCATTTCTCTCCCTCGTGTCGCCCAAGATCAGTATGACGCTA
CGCAAGAAATCTCTATGGAAGAAGCCCAAGCAGGCGACCTTATCTTCTTTCATTCAACCT
ATAATGCAGGCACATATGTGACTCATGTGGCGATATATTTAGAGGGAAATCGCTTTTATC
ATGCAGGCGACCCAATCGGTTATGGCGACTTAAGCAGTCGTTATTGGCAAGACCATCTGA
TTGGGGCTAGACGTGTTATTCATAACTAACGCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

DDTDSGENNKDSSIPQGGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTA
EDVMQSSESLGLPPNSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNY
VRSHGKKYTYELAEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDT
SPTEFDDETVQVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQ
YDATQEISMEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQ
DHLIGARRVIHN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 417449
Recommended expression in:
Not Applicable