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KLHL6 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL6
Gene Name:  kelch-like family member 6
Sequence             
Original Clone ID: FLH269149.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329414

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1833nts         Open reading frame : 1 to 1833
Reference Sequence Alignment

ATGGGTGATGTGGTCGAGAAGAGTTTGGAAGGGCCCCTGGCACCTTCTACAGATGAGCCC
TCCCAGAAAACAGGAGACTTGGTCGAGATCTTAAATGGGGAAAAGGTCAAATTTGACGAC
GCGGGACTCTCCTTAATTCTTCAGAATGGCCTGGAAACCCTGCGAATGGAAAACGCTCTG
ACAGATGTCATCTTGTGTGTGGACATTCAGGAATTCTCCTGCCACCGCGTGGTGCTTGCC
GCAGCCAGCAACTATTTCAGGGCCATGTTCTGCAACGATTTAAAGGAAAAGTATGAGAAA
AGGATCATTATTAAAGGGGTTGATGCTGAGACCATGCACACTCTGTTGGACTACACGTAC
ACCAGCAAGGCGCTGATCACCAAGCAGAATGTCCAGCGGGTCCTGGAGGCTGCTAACCTC
TTCCAGTTCCTGCGGATGGTGGATGCCTGTGCCAGCTTCCTCACTGAAGCCTTGAACCCA
GAAAACTGCGTTGGAATACTGAGGCTGGCTGACACACACTCGCTGGACAGTCTAAAGAAG
CAGGTTCAGAGTTACATCATTCAAAACTTTGTGCAGATTCTGAACTCTGAGGAGTTTCTT
GACCTGCCCGTGGACACTCTGCACCACATCTTGAAGAGTGATGACCTTTACGTGACCGAG
GAGGCTCAGGTGTTTGAGACCGTGATGAGCTGGGTCCGGCACAAGCCATCAGAACGACTC
TGCTTACTCCCCTATGTCCTCGAGAACGTGCGCTTACCGCTTCTGGACCCGTGGTACTTT
GTGGAGACGGTGGAAGCAGATCCTCTCATCAGGCAGTGCCCAGAGGTCTTCCCGCTGCTC
CAGGAAGCCAGGATGTACCACCTTTCTGGCAATGAGATCATTTCGGAACGCACCAAGCCC
AGGATGCATGAGTTCCAGTCTGAGGTGTTCATGATCATTGGCGGCTGCACGAAGGATGAA
CGGTTTGTGGCAGAGGTGACCTGCCTGGACCCCCTGAGGCGCAGCCGCCTGGAGGTGGCC
AAGCTCCCGCTAACAGAGCATGAGCTGGAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCA
TGCGTGACATTGAAAAATGAGGTCTACATCTCAGGTGGCAAAGAAACACAGCATGATGTT
TGGAAATATAATTCTTCGATCAACAAGTGGATTCAGATTGAGTATTTAAACATAGGCCGC
TGGAGGCATAAGATGGTGGTGTTGGGTGGCAAGGTCTATGTGATCGGAGGCTTTGACGGC
TTACAGAGAATCAACAACGTGGAGACCTACGACCCCTTTCACAACTGCTGGTCAGAGGCC
GCACCCCTCCTTGTCCATGTCAGTTCCTTTGCAGCCACCAGCCATAAGAAGAAGCTGTAT
GTGATCGGGGGAGGGCCCAATGGGAAACTGGCCACAGACAAGACTCAGTGTTATGACCCT
TCCACCAACAAGTGGAGTTTGAAGGCGGCCATGCCCGTGGAGGCTAAATGCATCAATGCA
GTGAGTTTCCGGGACCGCATCTATGTCGTTGGTGGGGCCATGAGAGCGCTGTACGCCTAC
AGCCCGCTGGAAGACAGCTGGTGCCTGGTGACCCAGCTCAGCCACGAGCGGGCCAGCTGC
GGTATCGCGCCCTGCAACAACCGGCTCTACATCACCGGCGGGCGGGACGAGAAGAACGAG
GTTATCGCCACGGTGCTGTGCTGGGACCCCGAGGCCCAGAAACTGACAGAGGAGTGCGTC
CTGCCCCGGGGCGTGTCGCACCACGGCAGCGTCACCATCAGGAAGTCGTACACCCACATC
CGCAGGATCGTGCCCGGAGCAGTGTCTGTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1902
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL6
Symbol Nomenclature : KLHL6
Designation : kelch-like 6|kelch-like protein 6|kelch-like protein KLHL6
Full Nomenclature : kelch-like family member 6
GENEID : 89857
HGNC : 18653
MIM : 614214
Vega : OTTHUMG00000148673
Target GenBank: BC032348

 

Reference Sequence Alignment

  

HsCD00329414      ---------------------------------ATGGGTGATGTGGTCGAGAAGAGTTTG
NM_130446.2       ATGTTGATGGCAGGACAAAGGGGCGCCTGGACCATGGGTGATGTGGTCGAGAAGAGTTTG
                                                   ***************************

HsCD00329414      GAAGGGCCCCTGGCACCTTCTACAGATGAGCCCTCCCAGAAAACAGGAGACTTGGTCGAG
NM_130446.2       GAAGGGCCCCTGGCACCTTCTACAGATGAGCCCTCCCAGAAAACAGGAGACTTGGTCGAG
                  ************************************************************

HsCD00329414      ATCTTAAATGGGGAAAAGGTCAAATTTGACGACGCGGGACTCTCCTTAATTCTTCAGAAT
NM_130446.2       ATCTTAAATGGGGAAAAGGTCAAATTTGACGACGCGGGACTCTCCTTAATTCTTCAGAAT
                  ************************************************************

HsCD00329414      GGCCTGGAAACCCTGCGAATGGAAAACGCTCTGACAGATGTCATCTTGTGTGTGGACATT
NM_130446.2       GGCCTGGAAACCCTGCGAATGGAAAACGCTCTGACAGATGTCATCTTGTGTGTGGACATT
                  ************************************************************

HsCD00329414      CAGGAATTCTCCTGCCACCGCGTGGTGCTTGCCGCAGCCAGCAACTATTTCAGGGCCATG
NM_130446.2       CAGGAATTCTCCTGCCACCGCGTGGTGCTTGCCGCAGCCAGCAACTATTTCAGGGCCATG
                  ************************************************************

HsCD00329414      TTCTGCAACGATTTAAAGGAAAAGTATGAGAAAAGGATCATTATTAAAGGGGTTGATGCT
NM_130446.2       TTCTGCAACGATTTAAAGGAAAAGTATGAGAAAAGGATCATTATTAAAGGGGTTGATGCT
                  ************************************************************

HsCD00329414      GAGACCATGCACACTCTGTTGGACTACACGTACACCAGCAAGGCGCTGATCACCAAGCAG
NM_130446.2       GAGACCATGCACACTCTGTTGGACTACACGTACACCAGCAAGGCGCTGATCACCAAGCAG
                  ************************************************************

HsCD00329414      AATGTCCAGCGGGTCCTGGAGGCTGCTAACCTCTTCCAGTTCCTGCGGATGGTGGATGCC
NM_130446.2       AATGTCCAGCGGGTCCTGGAGGCTGCTAACCTCTTCCAGTTCCTGCGGATGGTGGATGCC
                  ************************************************************

HsCD00329414      TGTGCCAGCTTCCTCACTGAAGCCTTGAACCCAGAAAACTGCGTTGGAATACTGAGGCTG
NM_130446.2       TGTGCCAGCTTCCTCACTGAAGCCTTGAACCCAGAAAACTGCGTTGGAATACTGAGGCTG
                  ************************************************************

HsCD00329414      GCTGACACACACTCGCTGGACAGTCTAAAGAAGCAGGTTCAGAGTTACATCATTCAAAAC
NM_130446.2       GCTGACACACACTCGCTGGACAGTCTAAAGAAGCAGGTTCAGAGTTACATCATTCAAAAC
                  ************************************************************

HsCD00329414      TTTGTGCAGATTCTGAACTCTGAGGAGTTTCTTGACCTGCCCGTGGACACTCTGCACCAC
NM_130446.2       TTTGTGCAGATTCTGAACTCTGAGGAGTTTCTTGACCTGCCCGTGGACACTCTGCACCAC
                  ************************************************************

HsCD00329414      ATCTTGAAGAGTGATGACCTTTACGTGACCGAGGAGGCTCAGGTGTTTGAGACCGTGATG
NM_130446.2       ATCTTGAAGAGTGATGACCTTTACGTGACCGAGGAGGCTCAGGTGTTTGAGACCGTGATG
                  ************************************************************

HsCD00329414      AGCTGGGTCCGGCACAAGCCATCAGAACGACTCTGCTTACTCCCCTATGTCCTCGAGAAC
NM_130446.2       AGCTGGGTCCGGCACAAGCCATCAGAACGACTCTGCTTACTCCCCTATGTCCTCGAGAAC
                  ************************************************************

HsCD00329414      GTGCGCTTACCGCTTCTGGACCCGTGGTACTTTGTGGAGACGGTGGAAGCAGATCCTCTC
NM_130446.2       GTGCGCTTACCGCTTCTGGACCCGTGGTACTTTGTGGAGACGGTGGAAGCAGATCCTCTC
                  ************************************************************

HsCD00329414      ATCAGGCAGTGCCCAGAGGTCTTCCCGCTGCTCCAGGAAGCCAGGATGTACCACCTTTCT
NM_130446.2       ATCAGGCAGTGCCCAGAGGTCTTCCCGCTGCTCCAGGAAGCCAGGATGTACCACCTTTCT
                  ************************************************************

HsCD00329414      GGCAATGAGATCATTTCGGAACGCACCAAGCCCAGGATGCATGAGTTCCAGTCTGAGGTG
NM_130446.2       GGCAATGAGATCATTTCGGAACGCACCAAGCCCAGGATGCATGAGTTCCAGTCTGAGGTG
                  ************************************************************

HsCD00329414      TTCATGATCATTGGCGGCTGCACGAAGGATGAACGGTTTGTGGCAGAGGTGACCTGCCTG
NM_130446.2       TTCATGATCATTGGCGGCTGCACGAAGGATGAACGGTTTGTGGCAGAGGTGACCTGCCTG
                  ************************************************************

HsCD00329414      GACCCCCTGAGGCGCAGCCGCCTGGAGGTGGCCAAGCTCCCGCTAACAGAGCATGAGCTG
NM_130446.2       GACCCCCTGAGGCGCAGCCGCCTGGAGGTGGCCAAGCTCCCGCTAACAGAGCATGAGCTG
                  ************************************************************

HsCD00329414      GAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCATGCGTGACATTGAAAAATGAGGTCTAC
NM_130446.2       GAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCATGCGTGACATTGAAAAATGAGGTCTAC
                  ************************************************************

HsCD00329414      ATCTCAGGTGGCAAAGAAACACAGCATGATGTTTGGAAATATAATTCTTCGATCAACAAG
NM_130446.2       ATCTCAGGTGGCAAAGAAACACAGCATGATGTTTGGAAATATAATTCTTCGATCAACAAG
                  ************************************************************

HsCD00329414      TGGATTCAGATTGAGTATTTAAACATAGGCCGCTGGAGGCATAAGATGGTGGTGTTGGGT
NM_130446.2       TGGATTCAGATTGAGTATTTAAACATAGGCCGCTGGAGGCATAAGATGGTGGTGTTGGGT
                  ************************************************************

HsCD00329414      GGCAAGGTCTATGTGATCGGAGGCTTTGACGGCTTACAGAGAATCAACAACGTGGAGACC
NM_130446.2       GGCAAGGTCTATGTGATCGGAGGCTTTGACGGCTTACAGAGAATCAACAATGTGGAGACC
                  ************************************************** *********

HsCD00329414      TACGACCCCTTTCACAACTGCTGGTCAGAGGCCGCACCCCTCCTTGTCCATGTCAGTTCC
NM_130446.2       TACGACCCCTTTCACAACTGCTGGTCAGAGGCCGCACCCCTCCTTGTCCATGTCAGTTCC
                  ************************************************************

HsCD00329414      TTTGCAGCCACCAGCCATAAGAAGAAGCTGTATGTGATCGGGGGAGGGCCCAATGGGAAA
NM_130446.2       TTTGCAGCCACCAGCCATAAGAAGAAGCTGTATGTGATCGGGGGAGGGCCCAATGGGAAA
                  ************************************************************

HsCD00329414      CTGGCCACAGACAAGACTCAGTGTTATGACCCTTCCACCAACAAGTGGAGTTTGAAGGCG
NM_130446.2       CTGGCCACAGACAAGACTCAGTGTTATGACCCTTCCACCAACAAGTGGAGTTTGAAGGCG
                  ************************************************************

HsCD00329414      GCCATGCCCGTGGAGGCTAAATGCATCAATGCAGTGAGTTTCCGGGACCGCATCTATGTC
NM_130446.2       GCCATGCCCGTGGAGGCTAAATGCATCAATGCAGTGAGTTTCCGGGACCGCATCTATGTC
                  ************************************************************

HsCD00329414      GTTGGTGGGGCCATGAGAGCGCTGTACGCCTACAGCCCGCTGGAAGACAGCTGGTGCCTG
NM_130446.2       GTTGGTGGGGCCATGAGAGCGCTGTACGCCTACAGCCCGCTGGAAGACAGCTGGTGCCTG
                  ************************************************************

HsCD00329414      GTGACCCAGCTCAGCCACGAGCGGGCCAGCTGCGGTATCGCGCCCTGCAACAACCGGCTC
NM_130446.2       GTGACCCAGCTCAGCCACGAGCGGGCCAGCTGCGGTATCGCGCCCTGCAACAACCGGCTC
                  ************************************************************

HsCD00329414      TACATCACCGGCGGGCGGGACGAGAAGAACGAGGTTATCGCCACGGTGCTGTGCTGGGAC
NM_130446.2       TACATCACCGGCGGGCGGGACGAGAAGAACGAGGTTATCGCCACGGTGCTGTGCTGGGAC
                  ************************************************************

HsCD00329414      CCCGAGGCCCAGAAACTGACAGAGGAGTGCGTCCTGCCCCGGGGCGTGTCGCACCACGGC
NM_130446.2       CCCGAGGCCCAGAAACTGACAGAGGAGTGCGTCCTGCCCCGGGGCGTGTCGCACCACGGC
                  ************************************************************

HsCD00329414      AGCGTCACCATCAGGAAGTCGTACACCCACATCCGCAGGATCGTGCCCGGAGCAGTGTCT
NM_130446.2       AGCGTCACCATCAGGAAGTCGTACACCCACATCCGCAGGATCGTGCCCGGAGCAGTGTCT
                  ************************************************************

HsCD00329414      GTCTTG
NM_130446.2       GTCTGA
                  **** .

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : Kelch :
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
UniProt : B7Z5I9
UniProt : Q8IXQ5
UniProt : A8K364
HPRD : 10035

Clone

Cloning Information : 269149
HIP Master Clone ID : 153551
Original Clone ID : FLH269149.01L

Organism


TAX_ID : 9606
Species Specific ID: 55975

Genome

Chromosome : 3
Map Location : 3q27.3
Ensembl : ENSG00000172578

Reagents

Labome : KLHL6-antibody