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L3MBTL2 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  L3MBTL2
Gene Name:  l(3)mbt-like 2 (Drosophila)
Sequence             
Original Clone ID: FLH269155.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329420

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2118nts         Open reading frame : 1 to 2118
Reference Sequence Alignment

ATGGAGAAGCCCCGGAGTATTGAGGAGACCCCATCTTCAGAACCAATGGAGGAAGAGGAA
GATGACGACTTGGAGCTGTTTGGTGGCTATGATAGTTTCCGGAGTTATAACAGCAGTGTG
GGCAGTGAGAGCAGCTCCTATCTGGAGGAGTCAAGTGAAGCAGAAAATGAGGATCGGGAA
GCAGGGGAACTGCCGACCTCCCCGCTGCATTTGCTCAGCCCTGGGACTCCTCGCTCCTTG
GATGGCAGTGGTTCTGAGCCAGCTGTCTGTGAGATGTGTGGTATCGTGGGTACAAGGGAA
GCCTTCTTCTCCAAGACCAAGAGGTTCTGCAGCGTCTCCTGCTCCAGGAGCTACTCCTCC
AACTCCAAGAAAGCCAGTATCTTGGCTAGATTACAGGGAAAACCACCGACCAAAAAAGCC
AAAGTCCTGCACAAGGCTGCCTGGTCTGCCAAAATTGGAGCCTTCCTCCACTCTCAAGGG
ACAGGACAGCTGGCAGATGGGACACCAACAGGACAAGACGCTCTGGTCTTGGGCTTCGAC
TGGGGGAAGTTCCTGAAGGATCACAGTTACAAGGCTGCTCCCGTCAGCTGTTTCAAGCAC
GTCCCACTCTATGACCAGTGGGAGGATGTGATGAAAGGGATGAAGGTGGAGGTGCTCAAC
AGTGATGCTGTGCTCCCCAGCCGGGTGTACTGGATCGCCTCTGTCATCCAGACAGCAGGG
TATCGGGTGCTGCTTCGGTATGAAGGCTTTGAAAATGACGCCAGCCATGACTTCTGGTGC
AACCTGGGAACAGTGGATGTCCACCCCATTGGCTGGTGTGCCATCAACAGCAAGATCCTA
GTGCCCCCACGGACCATCCATGCCAAGTTCACCGACTGGAAGGGCTACCTCATGAAACGG
CTGGTGGGCTCCAGGACGCTTCCCGTGGATTTCCACATCAAGATGGTGGAGAGCATGAAG
TACCCCTTTAGGCAGGGCATGCGGCTGGAAGTGGTGGACAAGTCCCAGGTGTCACGCACT
CGCATGGCTGTGGTGGACACAGTAATCGGGGGTCGCCTACGGCTCCTCTACGAGGATGGT
GACAGTGACGACGACTTCTGGTGCCACATGTGGAGCCCCCTGATCCACCCAGTGGGTTGG
TCACGACGTGTGGGCCACGGCATCAAGATGTCAGAGAGGCGAAGTGACATGGCCCATCAC
CCCACCTTCCGGAAGATCTACTGTGATGCCGTTCCTTACCTCTTCAAGAAGGTACGAGCA
GTCTACACAGAAGGCGGTTGGTTTGAGGAAGGGATGAAGCTGGAGGCCATTGACCCCCTG
AATCTGGGCAACATCTGCGTGGCAACTGTCTGTAAGGTTCTCCTGGATGGATACCTGATG
ATCTGTGTGGACGGGGGGCCCTCCACAGATGGCTTGGACTGGTTCTGCTACCATGCCTCT
TCCCACGCCATCTTCCCGGCCACCTTCTGTCAGAAGAATGACATTGAGCTCACACCGCCA
AAAGGTTATGAGGCACAGACTTTCAACTGGGAGAACTACTTGGAGAAGACCAAGTCGAAA
GCCGCTCCATCGAGACTCTTTAACATGGATTGCCCAAACCATGGCTTCAAGGTGGGCATG
AAGCTGGAGGCCGTGGACCTGATGGAGCCCCGGCTCATCTGTGTGGCCACGGTGAAACGA
GTGGTGCATCGGCTCCTCAGCATCCACTTTGACGGCTGGGACAGCGAGTACGACCAGTGG
GTGGACTGCGAGTCCCCAGACATCTACCCCGTCGGCTGGTGTGAGCTCACCGGCTACCAG
CTCCAGCCTCCTGTGGCCGCAGAACCGGCCACACCGCTGAAGGCCAAAGAGGCCACAAAG
AAGAAAAAGAAACAGTTTGGGAAGAAAAGGAAAAGAATCCCGCCCACTAAGACGCGACCC
CTCAGACAGGGGTCCAAGAAGCCCCTGCTGGAGGACGACCCTCAGGGTGCCAGGAAGATC
TCGTCGGAGCCTGTTCCTGGCGAGATCATTGCTGTGCGTGTGAAGGAAGAGCATCTAGAC
GTGGCCTCGCCCGACAAGGCTTCAAGTCCAGAGCTGCCTGTCTCCGTCGAGAACATCAAG
CAGGAAACAGACGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2170
Start on reference sequence 53
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : L3MBTL2
Symbol Nomenclature : L3MBTL2
SYNONYM : H-l(3)mbt-l; L3MBT
Designation : H-l(3)mbt-like protein 2|L(3)mbt-like protein 2|lethal(3)malignant brain tumor-like protein 2
Full Nomenclature : l(3)mbt-like 2 (Drosophila)
GENEID : 83746
Locus Tag : RP4-756G23.4
HGNC : 18594
MIM : 611865
Vega : OTTHUMG00000150942
Target GenBank: BC017191

 

Reference Sequence Alignment

  

HsCD00329420      ATGGAGAAGCCCCGGAGTATTGAGGAGACCCCATCTTCAGAACCAATGGAGGAAGAGGAA
NM_031488.4       ATGGAGAAGCCCCGGAGTATTGAGGAGACCCCATCTTCAGAACCAATGGAGGAAGAGGAA
                  ************************************************************

HsCD00329420      GATGACGACTTGGAGCTGTTTGGTGGCTATGATAGTTTCCGGAGTTATAACAGCAGTGTG
NM_031488.4       GATGACGACTTGGAGCTGTTTGGTGGCTATGATAGTTTCCGGAGTTATAACAGCAGTGTG
                  ************************************************************

HsCD00329420      GGCAGTGAGAGCAGCTCCTATCTGGAGGAGTCAAGTGAAGCAGAAAATGAGGATCGGGAA
NM_031488.4       GGCAGTGAGAGCAGCTCCTATCTGGAGGAGTCAAGTGAAGCAGAAAATGAGGATCGGGAA
                  ************************************************************

HsCD00329420      GCAGGGGAACTGCCGACCTCCCCGCTGCATTTGCTCAGCCCTGGGACTCCTCGCTCCTTG
NM_031488.4       GCAGGGGAACTGCCGACCTCCCCGCTGCATTTGCTCAGCCCTGGGACTCCTCGCTCCTTG
                  ************************************************************

HsCD00329420      GATGGCAGTGGTTCTGAGCCAGCTGTCTGTGAGATGTGTGGTATCGTGGGTACAAGGGAA
NM_031488.4       GATGGCAGTGGTTCTGAGCCAGCTGTCTGTGAGATGTGTGGTATCGTGGGTACAAGGGAA
                  ************************************************************

HsCD00329420      GCCTTCTTCTCCAAGACCAAGAGGTTCTGCAGCGTCTCCTGCTCCAGGAGCTACTCCTCC
NM_031488.4       GCCTTCTTCTCCAAGACCAAGAGGTTCTGCAGCGTCTCCTGCTCCAGGAGCTACTCCTCC
                  ************************************************************

HsCD00329420      AACTCCAAGAAAGCCAGTATCTTGGCTAGATTACAGGGAAAACCACCGACCAAAAAAGCC
NM_031488.4       AACTCCAAGAAAGCCAGTATCTTGGCTAGGTTACAGGGAAAACCACCGACCAAAAAAGCC
                  *****************************.******************************

HsCD00329420      AAAGTCCTGCACAAGGCTGCCTGGTCTGCCAAAATTGGAGCCTTCCTCCACTCTCAAGGG
NM_031488.4       AAAGTCCTGCACAAGGCTGCCTGGTCTGCCAAAATTGGAGCCTTCCTCCACTCTCAAGGG
                  ************************************************************

HsCD00329420      ACAGGACAGCTGGCAGATGGGACACCAACAGGACAAGACGCTCTGGTCTTGGGCTTCGAC
NM_031488.4       ACAGGACAGCTGGCAGATGGGACACCAACAGGACAAGACGCTCTGGTCTTGGGCTTCGAC
                  ************************************************************

HsCD00329420      TGGGGGAAGTTCCTGAAGGATCACAGTTACAAGGCTGCTCCCGTCAGCTGTTTCAAGCAC
NM_031488.4       TGGGGGAAGTTCCTGAAGGATCACAGTTACAAGGCTGCTCCCGTCAGCTGTTTCAAGCAC
                  ************************************************************

HsCD00329420      GTCCCACTCTATGACCAGTGGGAGGATGTGATGAAAGGGATGAAGGTGGAGGTGCTCAAC
NM_031488.4       GTCCCACTCTATGACCAGTGGGAGGATGTGATGAAAGGGATGAAGGTGGAGGTGCTCAAC
                  ************************************************************

HsCD00329420      AGTGATGCTGTGCTCCCCAGCCGGGTGTACTGGATCGCCTCTGTCATCCAGACAGCAGGG
NM_031488.4       AGTGATGCTGTGCTCCCCAGCCGGGTGTACTGGATCGCCTCTGTCATCCAGACAGCAGGG
                  ************************************************************

HsCD00329420      TATCGGGTGCTGCTTCGGTATGAAGGCTTTGAAAATGACGCCAGCCATGACTTCTGGTGC
NM_031488.4       TATCGGGTGCTGCTTCGGTATGAAGGCTTTGAAAATGACGCCAGCCATGACTTCTGGTGC
                  ************************************************************

HsCD00329420      AACCTGGGAACAGTGGATGTCCACCCCATTGGCTGGTGTGCCATCAACAGCAAGATCCTA
NM_031488.4       AACCTGGGAACAGTGGATGTCCACCCCATTGGCTGGTGTGCCATCAACAGCAAGATCCTA
                  ************************************************************

HsCD00329420      GTGCCCCCACGGACCATCCATGCCAAGTTCACCGACTGGAAGGGCTACCTCATGAAACGG
NM_031488.4       GTGCCCCCACGGACCATCCATGCCAAGTTCACCGACTGGAAGGGCTACCTCATGAAACGG
                  ************************************************************

HsCD00329420      CTGGTGGGCTCCAGGACGCTTCCCGTGGATTTCCACATCAAGATGGTGGAGAGCATGAAG
NM_031488.4       CTGGTGGGCTCCAGGACGCTTCCCGTGGATTTCCACATCAAGATGGTGGAGAGCATGAAG
                  ************************************************************

HsCD00329420      TACCCCTTTAGGCAGGGCATGCGGCTGGAAGTGGTGGACAAGTCCCAGGTGTCACGCACT
NM_031488.4       TACCCCTTTAGGCAGGGCATGCGGCTGGAAGTGGTGGACAAGTCCCAGGTGTCACGCACT
                  ************************************************************

HsCD00329420      CGCATGGCTGTGGTGGACACAGTAATCGGGGGTCGCCTACGGCTCCTCTACGAGGATGGT
NM_031488.4       CGCATGGCTGTGGTGGACACAGTAATCGGGGGTCGCCTACGGCTCCTCTACGAGGATGGT
                  ************************************************************

HsCD00329420      GACAGTGACGACGACTTCTGGTGCCACATGTGGAGCCCCCTGATCCACCCAGTGGGTTGG
NM_031488.4       GACAGTGACGACGACTTCTGGTGCCACATGTGGAGCCCCCTGATCCACCCAGTGGGTTGG
                  ************************************************************

HsCD00329420      TCACGACGTGTGGGCCACGGCATCAAGATGTCAGAGAGGCGAAGTGACATGGCCCATCAC
NM_031488.4       TCACGACGTGTGGGCCACGGCATCAAGATGTCAGAGAGGCGAAGTGACATGGCCCATCAC
                  ************************************************************

HsCD00329420      CCCACCTTCCGGAAGATCTACTGTGATGCCGTTCCTTACCTCTTCAAGAAGGTACGAGCA
NM_031488.4       CCCACCTTCCGGAAGATCTACTGTGATGCCGTTCCTTACCTCTTCAAGAAGGTACGAGCA
                  ************************************************************

HsCD00329420      GTCTACACAGAAGGCGGTTGGTTTGAGGAAGGGATGAAGCTGGAGGCCATTGACCCCCTG
NM_031488.4       GTCTACACAGAAGGCGGTTGGTTTGAGGAAGGGATGAAGCTGGAGGCCATTGACCCCCTG
                  ************************************************************

HsCD00329420      AATCTGGGCAACATCTGCGTGGCAACTGTCTGTAAGGTTCTCCTGGATGGATACCTGATG
NM_031488.4       AATCTGGGCAACATCTGCGTGGCAACTGTCTGTAAGGTTCTCCTGGATGGATACCTGATG
                  ************************************************************

HsCD00329420      ATCTGTGTGGACGGGGGGCCCTCCACAGATGGCTTGGACTGGTTCTGCTACCATGCCTCT
NM_031488.4       ATCTGTGTGGACGGGGGGCCCTCCACAGATGGCTTGGACTGGTTCTGCTACCATGCCTCT
                  ************************************************************

HsCD00329420      TCCCACGCCATCTTCCCGGCCACCTTCTGTCAGAAGAATGACATTGAGCTCACACCGCCA
NM_031488.4       TCCCACGCCATCTTCCCGGCCACCTTCTGTCAGAAGAATGACATTGAGCTCACACCGCCA
                  ************************************************************

HsCD00329420      AAAGGTTATGAGGCACAGACTTTCAACTGGGAGAACTACTTGGAGAAGACCAAGTCGAAA
NM_031488.4       AAAGGTTATGAGGCACAGACTTTCAACTGGGAGAACTACTTGGAGAAGACCAAGTCGAAA
                  ************************************************************

HsCD00329420      GCCGCTCCATCGAGACTCTTTAACATGGATTGCCCAAACCATGGCTTCAAGGTGGGCATG
NM_031488.4       GCCGCTCCATCGAGACTCTTTAACATGGATTGCCCAAACCATGGCTTCAAGGTGGGCATG
                  ************************************************************

HsCD00329420      AAGCTGGAGGCCGTGGACCTGATGGAGCCCCGGCTCATCTGTGTGGCCACGGTGAAACGA
NM_031488.4       AAGCTGGAGGCCGTGGACCTGATGGAGCCCCGGCTCATCTGTGTGGCCACGGTGAAACGA
                  ************************************************************

HsCD00329420      GTGGTGCATCGGCTCCTCAGCATCCACTTTGACGGCTGGGACAGCGAGTACGACCAGTGG
NM_031488.4       GTGGTGCATCGGCTCCTCAGCATCCACTTTGACGGCTGGGACAGCGAGTACGACCAGTGG
                  ************************************************************

HsCD00329420      GTGGACTGCGAGTCCCCAGACATCTACCCCGTCGGCTGGTGTGAGCTCACCGGCTACCAG
NM_031488.4       GTGGACTGCGAGTCCCCAGACATCTACCCCGTCGGCTGGTGTGAGCTCACCGGCTACCAG
                  ************************************************************

HsCD00329420      CTCCAGCCTCCTGTGGCCGCAGAACCGGCCACACCGCTGAAGGCCAAAGAGGCCACAAAG
NM_031488.4       CTCCAGCCTCCTGTGGCCGCAGAACCGGCCACACCGCTGAAGGCCAAAGAGGCCACAAAG
                  ************************************************************

HsCD00329420      AAGAAAAAGAAACAGTTTGGGAAGAAAAGGAAAAGAATCCCGCCCACTAAGACGCGACCC
NM_031488.4       AAGAAAAAGAAACAGTTTGGGAAGAAAAGGAAAAGAATCCCGCCCACTAAGACGCGACCC
                  ************************************************************

HsCD00329420      CTCAGACAGGGGTCCAAGAAGCCCCTGCTGGAGGACGACCCTCAGGGTGCCAGGAAGATC
NM_031488.4       CTCAGACAGGGGTCCAAGAAGCCCCTGCTGGAGGACGACCCTCAGGGTGCCAGGAAGATC
                  ************************************************************

HsCD00329420      TCGTCGGAGCCTGTTCCTGGCGAGATCATTGCTGTGCGTGTGAAGGAAGAGCATCTAGAC
NM_031488.4       TCGTCGGAGCCTGTTCCTGGCGAGATCATTGCTGTGCGTGTGAAGGAAGAGCATCTAGAC
                  ************************************************************

HsCD00329420      GTGGCCTCGCCCGACAAGGCTTCAAGTCCAGAGCTGCCTGTCTCCGTCGAGAACATCAAG
NM_031488.4       GTGGCCTCGCCCGACAAGGCTTCAAGTCCAGAGCTGCCTGTCTCCGTCGAGAACATCAAG
                  ************************************************************

HsCD00329420      CAGGAAACAGACGACTTG
NM_031488.4       CAGGAAACAGACGACTGA
                  **************** .

Clone

Cloning Information : 269155
HIP Master Clone ID : 160067
Original Clone ID : FLH269155.01L

Organism


TAX_ID : 9606
Species Specific ID: 83746

Genome

Chromosome : 22
Map Location : 22q13.31-q13.33
Ensembl : ENSG00000100395

Reagents

Labome : L3MBTL2-antibody