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ORC5L (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ORC5
Gene Name:  origin recognition complex, subunit 5
Sequence             
Original Clone ID: FLH269156.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329421

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1308nts         Open reading frame : 1 to 1308
Reference Sequence Alignment

ATGCCCCACTTGGAAAACGTGGTGCTTTGTCGCGAGTCTCAAGTGTCCATCTTGCAGTCC
TTGTTTGGAGAGAGACATCATTTCAGCTTTCCATCCATTTTTATTTATGGACATACTGCT
AGTGGAAAGACCTATGTAACACAAACGTTGTTGAAAACTTTAGAGCTCCCACATGTGTTT
GTGAATTGTGTTGAATGCTTTACATTGAGGCTGCTTTTGGAACAAATTTTAAACAAATTG
AATCATCTTAGTTCTTCAGAGGATGGATGTTCTACTGAAATAACCTGTGAAACATTTAAT
GACTTTGTTCGCTTGTTTAAACAAGTAACCACAGCTGAAAATCTTAAAGATCAGACTGTA
TATATTGTTCTAGATAAAGCAGAGTATCTAAGAGATATGGAAGCAAATCTTTTGCCTGGA
TTTCTTAGATTACAAGAATTGGCTGACAGAAATGTGACTGTTCTCTTTCTCAGTGAAATT
GTTTGGGAAAAGTTTCGTCCAAATACTGGATGCTTTGAGCCGTTTGTCTTATATTTCCCT
GATTACAGCATAGGCAACCTTCAAAAGATCCTGTCCCATGATCATCCTCCAGAGTATTCA
GCTGATTTCTATGCTGCCTACATTAACATTCTTCTTGGAGTTTTCTACACTGTTTGTCGA
GATTTGAAAGAGCTCAGACATCTGGCAGTACTTAATTTTCCTAAATATTGTGAACCCGTG
GTTAAAGGAGAAGCAAGTGAACGTGATACTCGCAAACTGTGGAGAAATATTGAACCTCAT
TTGAAGAAAGCTATGCAGACTGTTTATCTCAGGGAAATATCAAGTTCCCAGTGGGAAAAG
CTACAGAAAGATGACACAGATCCGGGGCAACTGAAAGGCCTCTCAGCGCATACTCATGTG
GAACTTCCATATTACTCTAAGTTCATTCTAATTGCTGCATACCTTGCTTCATACAATCCA
GCAAGAACTGACAAGAGGTTTTTTCTTAAGCATCATGGAAAAATCAAGAAAACCAACTTT
CTAAAAAAACACGAAAAGACAAGCAATCATCTCCTTGGGCCAAAACCATTTCCACTAGAC
AGATTATTAGCAATATTATATAGTATCGTGGACAGCAGAGTTGCTCCAACAGCAAATATT
TTTTCCCAGATTACCTCTCTAGTGACCCTTCAGCTGTTAACCCTGGTTGGCCATGACGAT
CAGCTTGATGGACCAAAATACAAATGCACAGTGTCTCTAGACTTCATCAGAGCTATTGCA
AGGACGGTGAACTTTGACATAATAAAATACTTGTATGATTTCTTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1389
Start on reference sequence 82
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ORC5
Symbol Nomenclature : ORC5
SYNONYM : ORC5L; ORC5P; ORC5T
Designation : origin recognition complex subunit 5
Full Nomenclature : origin recognition complex, subunit 5
GENEID : 5001
HGNC : 8491
MIM : 602331
Vega : OTTHUMG00000157275
Target GenBank: BC023652

 

Reference Sequence Alignment

  

HsCD00329421      ATGCCCCACTTGGAAAACGTGGTGCTTTGTCGCGAGTCTCAAGTGTCCATCTTGCAGTCC
NM_002553.3       ATGCCCCACTTGGAAAACGTGGTGCTTTGTCGCGAGTCTCAAGTGTCCATCTTGCAGTCC
                  ************************************************************

HsCD00329421      TTGTTTGGAGAGAGACATCATTTCAGCTTTCCATCCATTTTTATTTATGGACATACTGCT
NM_002553.3       TTGTTTGGAGAGAGACATCATTTCAGCTTTCCATCCATTTTTATTTATGGACATACTGCT
                  ************************************************************

HsCD00329421      AGTGGAAAGACCTATGTAACACAAACGTTGTTGAAAACTTTAGAGCTCCCACATGTGTTT
NM_002553.3       AGTGGAAAGACCTATGTAACACAAACGTTGTTGAAAACTTTAGAGCTCCCACATGTGTTT
                  ************************************************************

HsCD00329421      GTGAATTGTGTTGAATGCTTTACATTGAGGCTGCTTTTGGAACAAATTTTAAACAAATTG
NM_002553.3       GTGAATTGTGTTGAATGCTTTACATTGAGGCTGCTTTTGGAACAAATTTTAAACAAATTG
                  ************************************************************

HsCD00329421      AATCATCTTAGTTCTTCAGAGGATGGATGTTCTACTGAAATAACCTGTGAAACATTTAAT
NM_002553.3       AATCATCTTAGTTCTTCAGAGGATGGATGTTCTACTGAAATAACCTGTGAAACATTTAAT
                  ************************************************************

HsCD00329421      GACTTTGTTCGCTTGTTTAAACAAGTAACCACAGCTGAAAATCTTAAAGATCAGACTGTA
NM_002553.3       GACTTTGTTCGCTTGTTTAAACAAGTAACCACAGCTGAAAATCTTAAAGATCAGACTGTA
                  ************************************************************

HsCD00329421      TATATTGTTCTAGATAAAGCAGAGTATCTAAGAGATATGGAAGCAAATCTTTTGCCTGGA
NM_002553.3       TATATTGTTCTAGATAAAGCAGAGTATCTAAGAGATATGGAAGCAAATCTTTTGCCTGGA
                  ************************************************************

HsCD00329421      TTTCTTAGATTACAAGAATTGGCTGACAGAAATGTGACTGTTCTCTTTCTCAGTGAAATT
NM_002553.3       TTTCTTAGATTACAAGAATTGGCTGACAGAAATGTGACTGTTCTCTTTCTCAGTGAAATT
                  ************************************************************

HsCD00329421      GTTTGGGAAAAGTTTCGTCCAAATACTGGATGCTTTGAGCCGTTTGTCTTATATTTCCCT
NM_002553.3       GTTTGGGAAAAGTTTCGTCCAAATACTGGATGCTTTGAGCCGTTTGTCTTATATTTCCCT
                  ************************************************************

HsCD00329421      GATTACAGCATAGGCAACCTTCAAAAGATCCTGTCCCATGATCATCCTCCAGAGTATTCA
NM_002553.3       GATTACAGCATAGGCAACCTTCAAAAGATCCTGTCCCATGATCATCCTCCAGAGTATTCA
                  ************************************************************

HsCD00329421      GCTGATTTCTATGCTGCCTACATTAACATTCTTCTTGGAGTTTTCTACACTGTTTGTCGA
NM_002553.3       GCTGATTTCTATGCTGCCTACATTAACATTCTTCTTGGAGTTTTCTACACTGTTTGTCGA
                  ************************************************************

HsCD00329421      GATTTGAAAGAGCTCAGACATCTGGCAGTACTTAATTTTCCTAAATATTGTGAACCCGTG
NM_002553.3       GATTTGAAAGAGCTCAGACATCTGGCAGTACTTAATTTTCCTAAATATTGTGAACCCGTG
                  ************************************************************

HsCD00329421      GTTAAAGGAGAAGCAAGTGAACGTGATACTCGCAAACTGTGGAGAAATATTGAACCTCAT
NM_002553.3       GTTAAAGGAGAAGCAAGTGAACGTGATACTCGCAAACTGTGGAGAAATATTGAACCTCAT
                  ************************************************************

HsCD00329421      TTGAAGAAAGCTATGCAGACTGTTTATCTCAGGGAAATATCAAGTTCCCAGTGGGAAAAG
NM_002553.3       TTGAAGAAAGCTATGCAGACTGTTTATCTCAGGGAAATATCAAGTTCCCAGTGGGAAAAG
                  ************************************************************

HsCD00329421      CTACAGAAAGATGACACAGATCCGGGGCAACTGAAAGGCCTCTCAGCGCATACTCATGTG
NM_002553.3       CTACAGAAAGATGACACAGATCCGGGGCAACTGAAAGGCCTCTCAGCGCATACTCATGTG
                  ************************************************************

HsCD00329421      GAACTTCCATATTACTCTAAGTTCATTCTAATTGCTGCATACCTTGCTTCATACAATCCA
NM_002553.3       GAACTTCCATATTACTCTAAGTTCATTCTAATTGCTGCATACCTTGCTTCATACAATCCA
                  ************************************************************

HsCD00329421      GCAAGAACTGACAAGAGGTTTTTTCTTAAGCATCATGGAAAAATCAAGAAAACCAACTTT
NM_002553.3       GCAAGAACTGACAAGAGGTTTTTTCTTAAGCATCATGGAAAAATCAAGAAAACCAACTTT
                  ************************************************************

HsCD00329421      CTAAAAAAACACGAAAAGACAAGCAATCATCTCCTTGGGCCAAAACCATTTCCACTAGAC
NM_002553.3       CTAAAAAAACACGAAAAGACAAGCAATCATCTCCTTGGGCCAAAACCATTTCCACTAGAC
                  ************************************************************

HsCD00329421      AGATTATTAGCAATATTATATAGTATCGTGGACAGCAGAGTTGCTCCAACAGCAAATATT
NM_002553.3       AGATTATTAGCAATATTATATAGTATCGTGGACAGCAGAGTTGCTCCAACAGCAAATATT
                  ************************************************************

HsCD00329421      TTTTCCCAGATTACCTCTCTAGTGACCCTTCAGCTGTTAACCCTGGTTGGCCATGACGAT
NM_002553.3       TTTTCCCAGATTACCTCTCTAGTGACCCTTCAGCTGTTAACCCTGGTTGGCCATGACGAT
                  ************************************************************

HsCD00329421      CAGCTTGATGGACCAAAATACAAATGCACAGTGTCTCTAGACTTCATCAGAGCTATTGCA
NM_002553.3       CAGCTTGATGGACCAAAATACAAATGCACAGTGTCTCTAGACTTCATCAGAGCTATTGCA
                  ************************************************************

HsCD00329421      AGGACGGTGAACTTTGACATAATAAAATACTTGTATGATTTCTTGTTG
NM_002553.3       AGGACGGTGAACTTTGACATAATAAAATACTTGTATGATTTCTTGTGA
                  ********************************************** .

Pathways

Reactome : Activation of ATR in response to replication stress
Reactome : Activation of the pre-replicative complex
Reactome : Assembly of the ORC complex at the origin of replication
Reactome : Assembly of the pre-replicative complex
Reactome : Association of licensing factors with the pre-replicative complex
Reactome : CDC6 association with the ORC:origin complex
Reactome : CDT1 association with the CDC6:ORC:origin complex
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : DNA Replication
Reactome : DNA Replication Pre-Initiation
Reactome : E2F mediated regulation of DNA replication
Reactome : E2F-enabled inhibition of pre-replication complex formation
Reactome : G1/S Transition
Reactome : G2/M Checkpoints
Reactome : M/G1 Transition
Reactome : Mitotic G1-G1/S phases
Reactome : Mitotic M-M/G1 phases
Reactome : Orc1 removal from chromatin
Reactome : Regulation of DNA replication
Reactome : Removal of licensing factors from origins
Reactome : S Phase
Reactome : Switching of origins to a post-replicative state
Reactome : Synthesis of DNA
WikiPathway : Cell cycle
WikiPathway : DNA Replication
WikiPathway : G1 to S cell cycle control

Protein

UniProt : Q53FC8
UniProt : O95268
UniProt : A4D0P7
UniProt : O43913
UniProt : A4D0P8
HPRD : 09086

Clone

Cloning Information : 269156
HIP Master Clone ID : 159715
Original Clone ID : FLH269156.01L

Organism


TAX_ID : 9606
Species Specific ID: 5001

Genome

Chromosome : 7
Map Location : 7q22.1
Ensembl : ENSG00000164815

Reagents

Labome : ORC5-antibody