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ABCF2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ABCF2
Gene Name:  ATP-binding cassette, sub-family F (GCN20), member 2
Sequence             
Original Clone ID: FLH269157.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329422

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1872nts         Open reading frame : 1 to 1872
Reference Sequence Alignment

ATGCCCTCCGACTTGGCCAAGAAGAAGGCAGCCAAAAAGAAGGAGGCTGCCAAAGCTCGA
CAGCGGCCCAGAAAAGGACATGAAGAAAATGGAGATGTTGTCACAGAACCACAGGTGGCA
GAGAAGAATGAGGCCAATGGCAGAGAGACCACAGAAGTAGATTTGCTGACCAAGGAGCTA
GAGGACTTTGAGATGAAGAAAGCTGCTGCTCGAGCTGTCACTGGCGTCCTGGCCTCTCAC
CCCAACAGTACTGATGTTCACATCATCAACCTCTCACTCACCTTTCATGGTCAAGAGCTG
CTCAGTGACACCAAACTGGAATTAAACTCAGGCCGTCGTTATGGCCTCATTGGTTTAAAT
GGAATTGGAAAGTCCATGCTGCTCTCTGCTATTGGGAAGCGTGAAGTGCCCATCCCTGAG
CACATCGACATCTACCATCTGACTCGAGAGATGCCCCCTAGTGACAAGACACCCTTGCAT
TGTGTGATGGAAGTCGACACAGAGCGGGCCATGCTGGAGAAAGAGGCAGAGCGGCTGGCT
CATGAGGATGCGGAGTGTGAGAAGCTCATGGAGCTCTACGAGCGCCTGGAGGAGCTGGAT
GCCGACAAGGCAGAGATGAGGGCCTCGCGGATCTTGCATGGACTGGGTTTCACACCTGCC
ATGCAGCGCAAGAAGCTAAAAGACTTCAGTGGGGGCTGGAGGATGAGGGTTGCCCTTGCC
AGAGCCCTCTTTATTCGGCCCTTCATGCTGCTCCTGGATGAGCCTACCAACCACCTGGAC
CTAGATGCTTGCGTGTGGTTGGAAGAAGAACTAAAAACTTTTAAGCGCATCTTGGTCCTC
GTCTCCCATTCCCAGGATTTTCTGAATGGTGTCTGTACCAATATCATTCACATGCACAAC
AAGAAACTGAAGTATTATACGGGTAATTATGATCAGTATGTGAAGACGCGGCTAGAGCTG
GAGGAGAACCAGATGAAGAGGTTTCACTGGGAGCAAGATCAGATTGCACACATGAAGAAC
TACATTGCGAGGTTTGGTCATGGCAGTGCCAAGCTGGCCCGGCAGGCCCAGAGCAAGGAG
AAGACGCTACAGAAAATGATGGCATCAGGACTGACAGAGAGGGTCGTGAGCGATAAGACA
CTGTCATTTTATTTCCCACCATGTGGCAAGATCCCTCCACCTGTCATTATGGTGCAAAAT
GTGAGCTTCAAGTATACAAAAGATGGGCCTTGCATCTACAATAATCTAGAATTTGGAATT
GACCTTGACACACGAGTGGCTCTGGTAGGGCCCAATGGAGCAGGGAAGTCAACTCTTCTG
AAGCTGCTAACTGGAGAGCTACTACCCACAGATGGCATGATCCGAAAACACTCTCATGTC
AAGATAGGGCGTTACCATCAGCATTTACAAGAGCAGCTGGACTTAGATCTCTCACCTTTG
GAGTACATGATGAAGTGCTACCCAGAGATCAAGGAGAAGGAAGAAATGAGGAAGATCATT
GGGCGATACGGTCTCACTGGGAAACAACAGGTGAGCCCAATCCGGAACTTGTCAGACGGG
CAGAAGTGCCGAGTGTGTCTGGCCTGGCTGGCCTGGCAGAACCCCCACATGCTCTTCCTG
GATGAACCCACCAATCACCTGGATATCGAGACCATCGACGCCCTGGCAGATGCCATCAAT
GAGTTTGAGGGTGGTATGATGCTGGTCAGCCATGACTTCAGACTCATTCAGCAGGTTGCA
CAGGAAATTTGGGTCTGTGAGAAGCAGACAATCACCAAGTGGCCTGGAGACATCCTGGCT
TACAAGGAGCACCTCAAGTCCAAGCTGGTGGATGAGGAGCCCCAGCTCACCAAGAGGACC
CACAACGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1967
Start on reference sequence 96
Mutations:
None
Discrepancy:
c13t
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ABCF2
Symbol Nomenclature : ABCF2
SYNONYM : ABC28; EST133090; HUSSY18
Designation : ABC-type transport protein|ATP-binding cassette sub-family F member 2|iron-inhibited ABC transporter 2
Full Nomenclature : ATP-binding cassette, sub-family F (GCN20), member 2
GENEID : 10061
Locus Tag : HUSSY-18
HGNC : 71
MIM : 612510
Vega : OTTHUMG00000154570
Target GenBank: BC001661

 

Reference Sequence Alignment

  

HsCD00329422      ATGCCCTCCGACTTGGCCAAGAAGAAGGCAGCCAAAAAGAAGGAGGCTGCCAAAGCTCGA
NM_007189.2       ATGCCCTCCGACCTGGCCAAGAAGAAGGCAGCCAAAAAGAAGGAGGCTGCCAAAGCTCGA
NM_005692.4       ATGCCCTCCGACCTGGCCAAGAAGAAGGCAGCCAAAAAGAAGGAGGCTGCCAAAGCTCGA
                  ************ ***********************************************

HsCD00329422      CAGCGGCCCAGAAAAGGACATGAAGAAAATGGAGATGTTGTCACAGAACCACAGGTGGCA
NM_007189.2       CAGCGGCCCAGAAAAGGACATGAAGAAAATGGAGATGTTGTCACAGAACCACAGGTGGCA
NM_005692.4       CAGCGGCCCAGAAAAGGACATGAAGAAAATGGAGATGTTGTCACAGAACCACAGGTGGCA
                  ************************************************************

HsCD00329422      GAGAAGAATGAGGCCAATGGCAGAGAGACCACAGAAGTAGATTTGCTGACCAAGGAGCTA
NM_007189.2       GAGAAGAATGAGGCCAATGGCAGAGAGACCACAGAAGTAGATTTGCTGACCAAGGAGCTA
NM_005692.4       GAGAAGAATGAGGCCAATGGCAGAGAGACCACAGAAGTAGATTTGCTGACCAAGGAGCTA
                  ************************************************************

HsCD00329422      GAGGACTTTGAGATGAAGAAAGCTGCTGCTCGAGCTGTCACTGGCGTCCTGGCCTCTCAC
NM_007189.2       GAGGACTTTGAGATGAAGAAAGCTGCTGCTCGAGCTGTCACTGGCGTCCTGGCCTCTCAC
NM_005692.4       GAGGACTTTGAGATGAAGAAAGCTGCTGCTCGAGCTGTCACTGGCGTCCTGGCCTCTCAC
                  ************************************************************

HsCD00329422      CCCAACAGTACTGATGTTCACATCATCAACCTCTCACTCACCTTTCATGGTCAAGAGCTG
NM_007189.2       CCCAACAGTACTGATGTTCACATCATCAACCTCTCACTTACCTTTCATGGTCAAGAGCTG
NM_005692.4       CCCAACAGTACTGATGTTCACATCATCAACCTCTCACTTACCTTTCATGGTCAAGAGCTG
                  ************************************** *********************

HsCD00329422      CTCAGTGACACCAAACTGGAATTAAACTCAGGCCGTCGTTATGGCCTCATTGGTTTAAAT
NM_007189.2       CTCAGTGACACCAAACTGGAATTAAACTCAGGCCGTCGTTATGGCCTCATTGGTTTAAAT
NM_005692.4       CTCAGTGACACCAAACTGGAATTAAACTCAGGCCGTCGTTATGGCCTCATTGGTTTAAAT
                  ************************************************************

HsCD00329422      GGAATTGGAAAGTCCATGCTGCTCTCTGCTATTGGGAAGCGTGAAGTGCCCATCCCTGAG
NM_007189.2       GGAATTGGAAAGTCCATGCTGCTCTCTGCTATTGGGAAGCGTGAAGTGCCCATCCCTGAG
NM_005692.4       GGAATTGGAAAGTCCATGCTGCTCTCTGCTATTGGGAAGCGTGAAGTGCCCATCCCTGAG
                  ************************************************************

HsCD00329422      CACATCGACATCTACCATCTGACTCGAGAGATGCCCCCTAGTGACAAGACACCCTTGCAT
NM_007189.2       CACATCGACATCTACCATCTGACTCGAGAGATGCCCCCTAGTGACAAGACACCCTTGCAT
NM_005692.4       CACATCGACATCTACCATCTGACTCGAGAGATGCCCCCTAGTGACAAGACACCCTTGCAT
                  ************************************************************

HsCD00329422      TGTGTGATGGAAGTCGACACAGAGCGGGCCATGCTGGAGAAAGAGGCAGAGCGGCTGGCT
NM_007189.2       TGTGTGATGGAAGTCGACACAGAGCGGGCCATGCTGGAGAAAGAGGCAGAGCGGCTGGCT
NM_005692.4       TGTGTGATGGAAGTCGACACAGAGCGGGCCATGCTGGAGAAAGAGGCAGAGCGGCTGGCT
                  ************************************************************

HsCD00329422      CATGAGGATGCGGAGTGTGAGAAGCTCATGGAGCTCTACGAGCGCCTGGAGGAGCTGGAT
NM_007189.2       CATGAGGATGCGGAGTGTGAGAAGCTCATGGAGCTCTACGAGCGCCTGGAGGAGCTGGAT
NM_005692.4       CATGAGGATGCGGAGTGTGAGAAGCTCATGGAGCTCTACGAGCGCCTGGAGGAGCTGGAT
                  ************************************************************

HsCD00329422      GCCGACAAGGCAGAGATGAGGGCCTCGCGGATCTTGCATGGACTGGGTTTCACACCTGCC
NM_007189.2       GCCGACAAGGCAGAGATGAGGGCCTCGCGGATCTTGCATGGACTGGGTTTCACACCTGCC
NM_005692.4       GCCGACAAGGCAGAGATGAGGGCCTCGCGGATCTTGCATGGACTGGGTTTCACACCTGCC
                  ************************************************************

HsCD00329422      ATGCAGCGCAAGAAGCTAAAAGACTTCAGTGGGGGCTGGAGGATGAGGGTTGCCCTTGCC
NM_007189.2       ATGCAGCGCAAGAAGCTAAAAGACTTCAGTGGGGGCTGGAGGATGAGGGTTGCCCTTGCC
NM_005692.4       ATGCAGCGCAAGAAGCTAAAAGACTTCAGTGGGGGCTGGAGGATGAGGGTTGCCCTTGCC
                  ************************************************************

HsCD00329422      AGAGCCCTCTTTATTCGGCCCTTCATGCTGCTCCTGGATGAGCCTACCAACCACCTGGAC
NM_007189.2       AGAGCCCTCTTTATTCGGCCCTTCATGCTGCTCCTGGATGAGCCTACCAACCACCTGGAC
NM_005692.4       AGAGCCCTCTTTATTCGGCCCTTCATGCTGCTCCTGGATGAGCCTACCAACCACCTGGAC
                  ************************************************************

HsCD00329422      CTAGATGCTTGCGTGTGGTTGGAAGAAGAACTAAAAACTTTTAAGCGCATCTTGGTCCTC
NM_007189.2       CTAGATGCTTGCGTGTGGTTGGAAGAAGAACTAAAAACTTTTAAGCGCATCTTGGTCCTC
NM_005692.4       CTAGATGCTTGCGTGTGGTTGGAAGAAGAACTAAAAACTTTTAAGCGCATCTTGGTCCTC
                  ************************************************************

HsCD00329422      GTCTCCCATTCCCAGGATTTTCTGAATGGTGTCTGTACCAATATCATTCACATGCACAAC
NM_007189.2       GTCTCCCATTCCCAGGATTTTCTGAATGGTGTCTGTACCAATATCATTCACATGCACAAC
NM_005692.4       GTCTCCCATTCCCAGGATTTTCTGAATGGTGTCTGTACCAATATCATTCACATGCACAAC
                  ************************************************************

HsCD00329422      AAGAAACTGAAGTATTATACGGGTAATTATGATCAGTATGTGAAGACGCGGCTAGAGCTG
NM_007189.2       AAGAAACTGAAGTATTATACGGGTAATTATGATCAGTACGTGAAGACGCGGCTAGAGCTG
NM_005692.4       AAGAAACTGAAGTATTATACGGGTAATTATGATCAGTACGTGAAGACGCGGCTAGAGCTG
                  ************************************** *********************

HsCD00329422      GAGGAGAACCAGATGAAGAGGTTTCACTGGGAGCAAGATCAGATTGCACACATGAAGAAC
NM_007189.2       GAGGAGAACCAGATGAAGAGGTTTCACTGGGAGCAAGATCAGATTGCACACATGAAGAAC
NM_005692.4       GAGGAGAACCAGATGAAGAGGTTTCACTGGGAGCAAGATCAGATTGCACACATGAAGAAC
                  ************************************************************

HsCD00329422      TACATTGCGAGGTTTGGTCATGGCAGTGCCAAGCTGGCCCGGCAGGCCCAGAGCAAGGAG
NM_007189.2       TACATTGCGAGGTTTGGTCATGGCAGTGCCAAGCTGGCCCGGCAGGCCCAGAGCAAGGAG
NM_005692.4       TACATTGCGAGGTTTGGTCATGGCAGTGCCAAGCTGGCCCGGCAGGCCCAGAGCAAGGAG
                  ************************************************************

HsCD00329422      AAGACGCTACAGAAAATGATGGCATCAGGACTGACAGAGAGGGTCGTGAGCGATAAGACA
NM_007189.2       AAGACGCTACAGAAAATGATGGCATCAGGACTGACAGAGAGGGTCGTGAGCGATAAGACA
NM_005692.4       AAGACGCTACAGAAAATGATGGCATCAGGACTGACAGAGAGGGTCGTGAGCGATAAGACA
                  ************************************************************

HsCD00329422      CTGTCATTTTATTTCCCACCATGTGGCAAGATCCCTCCACCTGTCATTATGGTGCAAAAT
NM_007189.2       CTGTCATTTTATTTCCCACCATGTGGCAAGATCCCTCCACCTGTCATTATGGTGCAAAAT
NM_005692.4       CTGTCATTTTATTTCCCACCATGTGGCAAGATCCCTCCACCTGTCATTATGGTGCAAAAT
                  ************************************************************

HsCD00329422      GTGAGCTTCAAGTATACAAAAGATGGGCCTTGCATCTACAATAATCTAGAATTTGGAATT
NM_007189.2       GTGAGCTTCAAGTATACAAAAGATGGGCCTTGCATCTACAATAATCTAGAATTTGGAATT
NM_005692.4       GTGAGCTTCAAGTATACAAAAGATGGGCCTTGCATCTACAATAATCTAGAATTTGGAATT
                  ************************************************************

HsCD00329422      GACCTTGACACACGAGTGGCTCTGGTAGGGCCCAATGGAGCAGGGAAGTCAACTCTTCTG
NM_007189.2       GACCTTGACACACGAGTGGCTCTGGTAGGGCCCAATGGAGCAGGGAAGTCAACTCTTCTG
NM_005692.4       GACCTTGACACACGAGTGGCTCTGGTAGGGCCCAATGGAGCAGGGAAGTCAACTCTTCTG
                  ************************************************************

HsCD00329422      AAGCTGCTAACTGGAGAGCTACTACCCACAGATGGCATGATCCGAAAACACTCTCATGTC
NM_007189.2       AAGCTGCTAACTGGAGAGCTACTACCCACAGATGGCATGATCCGAAAACACTCTCATGTC
NM_005692.4       AAGCTGCTAACTGGAGAGCTACTACCCACAGATGGCATGATCCGAAAACACTCTCATGTC
                  ************************************************************

HsCD00329422      AAGATAGGGCGTTACCATCAGCATTTACAAGAGCAGCTGGACTTAGATCTCTCACCTTTG
NM_007189.2       AAGATAGGGCGTTACCATCAGCATTTACAAGAGCAGCTGGACTTAGATCTCTCACCTTTG
NM_005692.4       AAGATAGGGCGTTACCATCAGCATTTACAAGAGCAGCTGGACTTAGATCTCTCACCTTTG
                  ************************************************************

HsCD00329422      GAGTACATGATGAAGTGCTACCCAGAGATCAAGGAGAAGGAAGAAATGAGGAAGATCATT
NM_007189.2       GAGTACATGATGAAGTGCTACCCAGAGATCAAGGAGAAGGAAGAAATGAGGAAGATCATT
NM_005692.4       GAGTACATGATGAAGTGCTACCCAGAGATCAAGGAGAAGGAAGAAATGAGGAAGATCATT
                  ************************************************************

HsCD00329422      GGGCGATACGGTCTCACTGGGAAACAACAGGTGAGCCCAATCCGGAACTTGTCAGACGGG
NM_007189.2       GGGCGATACGGTCTCACTGGGAAACAACAGGTGAGCCCAATCCGGAACTTGTCAGACGGG
NM_005692.4       GGGCGATACGGTCTCACTGGGAAACAACAGGTGAGCCCAATCCGGAACTTGTCAGACGGG
                  ************************************************************

HsCD00329422      CAGAAGTGCCGAGTGTGTCTGGCCTGGCTGGCCTGGCAGAACCCCCACATGCTCTTCCTG
NM_007189.2       CAGAAGTGCCGAGTGTGTCTGGCCTGGCTGGCCTGGCAGAACCCCCACATGCTCTTCCTG
NM_005692.4       CAGAAGTGCCGAGTGTGTCTGGCCTGGCTGGCCTGGCAGAACCCCCACATGCTCTTCCTG
                  ************************************************************

HsCD00329422      GATGAACCCACCAATCACCTGGATATCGAGACCATCGACGCCCTGGCAGATGCCATCAAT
NM_007189.2       GATGAACCCACCAATCACCTGGATATCGAGACCATCGACGCCCTGGCAGATGCCATCAAT
NM_005692.4       GATGAACCCACCAATCACCTGGATATCGAGACCATCGACGCCCTGGCAGATGCCATCAAT
                  ************************************************************

HsCD00329422      GAGTTTGAGGGTGGTATGATGCTGGTCAGCCATGACTTCAGACTCATTCAGCAGGTTGCA
NM_007189.2       GAGTTTGAGGGTGGTATGATGCTGGTCAGCCATGACTTCAGACTCATTCAGCAGGTTGCA
NM_005692.4       GAGTTTGAGGGTGGTATGATGCTGGTCAGCCATGACTTCAGACTCATTCAGCAGGTTGCA
                  ************************************************************

HsCD00329422      CAGGAAATTTGGGTCTGTGAGAAGCAGACAATCACCAAGTGGCCTGGAGACATCCTGGCT
NM_007189.2       CAGGAAATTTGGGTCTGTGAGAAGCAGACAATCACCAAGTGGCCTGGAGACATCCTGGCT
NM_005692.4       CAGGAAATTTGGGTCTGTGAGAAGCAGACAATCACCAAGTGGCCTGGAGACATCCTGGCT
                  ************************************************************

HsCD00329422      TACAAGGAGCACCTCAAGTCCAAGCTGGTGGATGAGGAGCCCCAGCTCACCAAGAGGACC
NM_007189.2       TACAAGGAGCACCTCAAGTCCAAGCTGGTGGATGAGGAGCCCCAGCTCACCAAGAGGACC
NM_005692.4       TACAAGGAGCACCTCAAGTCCAAGCTGGTGGATGAGGAGCCCCAGCTCACCAAGAGGACC
                  ************************************************************

HsCD00329422      CACAACGTGTTG---------------------------------
NM_007189.2       CACAACGTGTGA---------------------------------
NM_005692.4       CACAACGTGTGCACCCTGACATTGGCATCTCTGCCAAGGCCATGA
                  **********                                   

Pathways

WikiPathway : Epithelium TarBase
WikiPathway : Lymphocyte TarBase

Protein

SMART domain : AAA : ATPases associated with a variety of cellular activities
UniProt : Q9UG63
UniProt : Q75MJ0
UniProt : Q75MJ1
HPRD : 12403

Clone

Cloning Information : 269157
HIP Master Clone ID : 159993
Original Clone ID : FLH269157.01L

Organism


TAX_ID : 9606
Species Specific ID: 10061

Genome

Chromosome : 7
Map Location : 7q36
Ensembl : ENSG00000033050

Reagents

Labome : ABCF2-antibody